Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,152,626	A→G	8.1%	R69R (CGA→CGG) ‡	PP_5738 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,152,626	0	A	G	8.1%	247.4 / 9.0	87	R69R (CGA→CGG) ‡	PP_5738	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (42/37);  new base G (4/3);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

AGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGACG  >  NC_002947/6152478‑6152774                                                                                                                                                     |                                                                                                                                                      aGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGa                                                                                                                                                       <  5:340274/149‑1 (MQ=255)    gATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                                    <  6:32149/149‑1 (MQ=255)    gATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                                    <  8:234762/149‑1 (MQ=255)      ttGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGa                                                                                                                                                  >  5:45590/1‑149 (MQ=255)         gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACTCGGTCGACat                                                                                                                                               <  8:325665/149‑2 (MQ=255)         gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAg                                                                                                                                               >  1:140768/1‑149 (MQ=255)            aGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAgg                                                                                                                                              >  6:221254/1‑147 (MQ=255)               ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                         >  5:95418/1‑149 (MQ=255)               ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                         >  5:90021/1‑149 (MQ=255)               ttGCCTGGCCAAGTCCGCGTATTTACCTACAACAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                         >  1:388573/1‑149 (MQ=255)                   cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTTTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                     >  1:112671/1‑149 (MQ=255)                   cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                     >  1:379607/1‑149 (MQ=255)                   cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGATGGCACTCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                     <  8:133320/149‑1 (MQ=255)                    tGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTg                                                                                                                                    >  8:269463/1‑149 (MQ=255)                      gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGc                                                                                                                                   <  6:288365/148‑1 (MQ=255)                      gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑g                                                                                                                                 <  7:206483/149‑1 (MQ=255)                       ccAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑gg                                                                                                                                <  4:14025/149‑1 (MQ=255)                       ccAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑gg                                                                                                                                >  3:100593/1‑149 (MQ=255)                        cAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGt                                                                                                                               <  2:112671/149‑1 (MQ=255)                                   aTTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCAc                                                                                                                    >  1:160952/1‑149 (MQ=255)                                       aCCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                     <  6:125242/129‑1 (MQ=255)                                          tACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑g                                                                                                                                 >  4:189216/1‑129 (MQ=255)                                              gcagAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAaagaa                                                                                                         <  2:165247/149‑1 (MQ=255)                                                agagagCTGGCACGCTTCGTTTCTTAGCGTGTAGTCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTTATAACGCGCCAAGATTTAGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAgg                                                                                                       <  1:251576/149‑1 (MQ=255)                                                agagagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAgg                                                                                                       >  8:95130/1‑149 (MQ=255)                                                 gagagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggc                                                                                                      <  4:100593/149‑1 (MQ=255)                                                   gagcgcgCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggcgg                                                                                                    <  8:244060/143‑1 (MQ=255)                                                   gagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggcg                                                                                                     <  7:269463/148‑1 (MQ=255)                                                      cttgCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCa                                                                                                  >  4:137560/4‑148 (MQ=255)                                                      cTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCaa                                                                                                 >  7:44716/1‑149 (MQ=255)                                                               gtcgtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCtcgtcgt                                                                                        >  7:70822/1‑149 (MQ=255)                                                                  gtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGa                                                                                     <  8:214795/149‑1 (MQ=255)                                                                      cTGAGCGTGTAGGCCATGTCACGCAAGACTATATGCCAGAGAAGTCCGCAGCTCTTATAACGCGCCAAGATGTCGACCGAGTCTACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGActc                                                                                  <  4:210020/148‑1 (MQ=255)                                                                      cTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGActc                                                                                  <  5:221254/148‑1 (MQ=255)                                                                         aGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTAGTCGACTCTCAc                                                                              >  5:393356/1‑149 (MQ=255)                                                                          gCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCAcc                                                                             <  6:45590/149‑1 (MQ=255)                                                                             tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                          <  4:12038/149‑1 (MQ=255)                                                                             tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                          <  2:379607/149‑1 (MQ=255)                                                                             tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                          <  7:50697/149‑1 (MQ=255)                                                                                  gCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAc                                                                      >  6:26450/1‑148 (MQ=255)                                                                                    cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                   >  3:373750/1‑149 (MQ=255)                                                                                    cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                   <  8:9286/149‑1 (MQ=255)                                                                                    cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                   >  3:171446/1‑149 (MQ=255)                                                                                     aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCATGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                   <  1:317212/148‑1 (MQ=255)                                                                                     aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCCGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCCCCCGGTGACGa                                                                    >  2:360932/1‑147 (MQ=255)                                                                                     aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                   >  2:317212/1‑148 (MQ=255)                                                                                          aCGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGaaa                                                              <  4:171446/148‑1 (MQ=255)                                                                                            gCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACAt                                                           >  4:16387/1‑149 (MQ=255)                                                                                              aaGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTc                                                         >  6:109303/1‑149 (MQ=255)                                                                                               aGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCg                                                        <  3:16387/149‑1 (MQ=255)                                                                                                gACGATATGCGAGAGAAGTCCGCAGCTATGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCgg                                                       >  1:210051/1‑149 (MQ=255)                                                                                                 aCGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGt                                                      <  2:320664/149‑1 (MQ=255)                                                                                                   gATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGtt                                                     >  7:121915/1‑148 (MQ=255)                                                                                                   gATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAAGATCGAAACATTCGGtt                                                     >  4:223581/1‑148 (MQ=255)                                                                                                     tatGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCa                                                  <  8:70822/149‑1 (MQ=255)                                                                                                       tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                                >  5:215525/1‑149 (MQ=255)                                                                                                       tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                                 <  8:121915/148‑1 (MQ=255)                                                                                                       tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                                 <  5:147773/148‑1 (MQ=255)                                                                                                       tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                                 <  2:160952/148‑1 (MQ=255)                                                                                                        gCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGAGGCAATCTCGTCGTCGACTCTCACCCCGTTACGATCGAAACATTCGGTTTCAggg                                               >  2:55400/1‑149 (MQ=255)                                                                                                         cGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                              >  5:87100/1‑149 (MQ=255)                                                                                                          gagagaAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                              >  2:196592/1‑148 (MQ=255)                                                                                                                   ccGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTCCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                    <  6:95418/149‑1 (MQ=255)                                                                                                                    cGCAGCTCTGATAACGCGCCAAGATGTCGACCGCGTCGACAGGGAGTTGATTGC‑GGTGGCGTCAGCATTCAAAAAAGACGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                    <  6:90021/148‑1 (MQ=255)                                                                                                                    cGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTg                                   <  8:44716/149‑1 (MQ=255)                                                                                                                            tGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgcg                           >  1:201834/1‑149 (MQ=255)                                                                                                                             gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                    >  6:379545/1‑139 (MQ=255)                                                                                                                             gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                    <  5:379545/139‑1 (MQ=255)                                                                                                                              aTAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgc                            <  6:393356/146‑1 (MQ=255)                                                                                                                               tAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑TGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCa                         <  1:55400/148‑1 (MQ=255)                                                                                                                               tAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAg                        >  4:156144/1‑149 (MQ=255)                                                                                                                                 aCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGtt                      >  6:257307/1‑149 (MQ=255)                                                                                                                                 aCGCGACAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTAACCCTGTTTCGATCGAAACAATCGGTTTCATGTTGACGCCATTTGCAAAAGCGCAttt                      >  8:89734/1‑149 (MQ=255)                                                                                                                                   gcgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTg                     >  8:231548/1‑148 (MQ=255)                                                                                                                                   gcgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTg                     >  8:190962/1‑148 (MQ=255)                                                                                                                                    cgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCa                   <  2:201834/149‑1 (MQ=255)                                                                                                                                    cgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCa                   <  6:87100/149‑1 (MQ=255)                                                                                                                                       cAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCACACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGa                >  1:340477/1‑149 (MQ=255)                                                                                                                                          cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                            >  6:143444/3‑118 (MQ=255)                                                                                                                                          cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                            <  5:143444/116‑1 (MQ=255)                                                                                                                                          cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt             >  2:201595/3‑149 (MQ=255)                                                                                                                                          cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt             >  6:222846/3‑149 (MQ=255)                                                                                                                                           ctGTCGACTCGGTCGACAGGGAGTTGATTGCGGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                                >  1:243531/2‑114 (MQ=255)                                                                                                                                           ctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                                <  2:243531/112‑1 (MQ=255)                                                                                                                                                gACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc        <  5:109303/148‑1 (MQ=255)                                                                                                                                                 aCCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc        >  7:209263/1‑147 (MQ=255)                                                                                                                                                  ccGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc        >  5:154389/1‑146 (MQ=255)                                                                                                                                                    gAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGAc   >  6:302721/1‑149 (MQ=255)                                                                                                                                                     aGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGACg  <  2:140768/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      AGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGACG  >  NC_002947/6152478‑6152774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑