BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	495,082	C→A	5.3%	E318* (GAG→TAG)	PP_0408 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	495,082	0	C	A	5.3%	348.5 / 6.7	112	E318* (GAG→TAG)	PP_0408	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (57/48); new base A (4/2); total (62/50)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 113 total aligned reads displayed.

CGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTAC  >  NC_002947/494936‑495229

cGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCtt                                                                                                                                                     <  4:63712/149‑1 (MQ=255)

 gTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTg                                                                                                                                                    >  2:175701/1‑149 (MQ=255)

 gTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTg                                                                                                                                                    >  2:244466/1‑149 (MQ=255)

 gTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTg                                                                                                                                                    >  3:243487/1‑149 (MQ=255)

     aGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTcc                                                                                                                                                <  5:228431/149‑1 (MQ=255)

             cgcgTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCttt                                                                                                                                        >  3:182046/1‑149 (MQ=255)

              gcgTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCACCGCACCCGGCGGAACAATTGCTCTTCTTCCGCCGCattg                                                                                                                                       >  3:62451/1‑149 (MQ=255)

               cgTACCCGTGAGTTGCACCAGGCCTATGCCATGATCGGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCtttgt                                                                                                                                      <  5:92493/149‑1 (MQ=255)

                     cGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtg                                                                                                                                >  2:281801/1‑149 (MQ=255)

                     cGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtg                                                                                                                                >  4:259523/1‑149 (MQ=255)

                      gTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtgg                                                                                                                               >  6:155474/1‑149 (MQ=255)

                      gTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtgg                                                                                                                               >  4:178373/1‑149 (MQ=255)

                         aGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggca                                                                                                                             <  7:205559/148‑1 (MQ=255)

                             gCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatggc                                                                                                                         >  3:8695/1‑148 (MQ=255)

                              cACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCtttgtt                                                                                                                                     <  4:7599/135‑1 (MQ=255)

                              cACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCtttgtt                                                                                                                                     >  3:7599/1‑135 (MQ=255)

                                 cAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatca                                                                                                                    <  1:175701/149‑1 (MQ=255)

                                 cAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatc                                                                                                                     >  5:101271/1‑148 (MQ=255)

                                 cAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatc                                                                                                                     >  6:135612/1‑148 (MQ=255)

                                  aGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatcaa                                                                                                                   >  2:333625/1‑149 (MQ=255)

                                  aGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatcaa                                                                                                                   >  7:4755/1‑149 (MQ=255)

                                     ccTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGa                                                                                                                <  7:362522/149‑1 (MQ=255)

                                     ccTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGa                                                                                                                <  3:120732/149‑1 (MQ=255)

                                     ccTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGa                                                                                                                <  3:155868/149‑1 (MQ=255)

                                           ccATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑g                                                                                                         <  4:310420/149‑1 (MQ=255)

                                           ccATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑g                                                                                                         >  5:98364/1‑149 (MQ=255)

                                            cATGATCCGCAAGCGGCGCGGCCTTTGACGGCCCCGCCCCAGGGATCACGCCTGCCTCCGCCGTGCGGGCTCATCCACCCCCGCCCCCGCCGGAACAATTGCTGTTGTTCCGCCGCTTTGTTTGGTGGCTTGGCAATCAATGACAATT‑g                                                                                                         >  1:29178/1‑149 (MQ=255)

                                            cATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTcc                                                                                                                                                <  3:19720/110‑1 (MQ=255)

                                            cATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTcc                                                                                                                                                >  4:19720/1‑110 (MQ=255)

                                             aTGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTc                                                                                                       >  3:215861/1‑149 (MQ=255)

                                              tGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCg                                                                                                      >  3:380266/1‑149 (MQ=255)

                                                aTCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGGTGGCATGGCAATCAATGACAATT‑GTCgg                                                                                                     <  1:218256/148‑1 (MQ=255)

                                                aTCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCgg                                                                                                     <  4:215861/148‑1 (MQ=255)

                                                aTCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGt                                                                                                    <  4:8695/149‑1 (MQ=255)

                                                    gCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTg                                                                                                >  6:262834/1‑149 (MQ=255)

                                                     cAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTgg                                                                                               <  4:243487/149‑1 (MQ=255)

                                                     cAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTgg                                                                                               <  1:281801/149‑1 (MQ=255)

                                                          ggcgcggcCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATc                                                                                          <  1:166654/149‑1 (MQ=255)

                                                             gcggcCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTTGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATc                                                                                          >  7:308539/1‑146 (MQ=255)

                                                             gcggcCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATc                                                                                          <  8:308539/146‑1 (MQ=255)

                                                               ggcCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCt                                                                                     <  8:325547/149‑1 (MQ=255)

                                                                 ccTTTGAC‑GCCCCGCCACAGGGTTCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGc                                                                                   <  3:259523/149‑1 (MQ=255)

                                                                   tttGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCg                                                                                 >  7:178323/1‑149 (MQ=255)

                                                                   tttGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCg                                                                                 >  7:183252/1‑149 (MQ=255)

                                                                          gCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCt                                                                           >  5:344723/1‑149 (MQ=255)

                                                                           ccccGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTg                                                                          <  8:4755/149‑1 (MQ=255)

                                                                            cccGCCACAGGGATCAAGCCTGACTCAGTCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTgg                                                                         >  3:23976/1‑149 (MQ=255)

                                                                             ccGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGc                                                                        >  8:329235/1‑149 (MQ=255)

                                                                             ccGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGc                                                                        >  3:261376/1‑149 (MQ=255)

                                                                             ccGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGc                                                                        >  3:183053/1‑149 (MQ=255)

                                                                                  acaGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTgg                                                                                               >  7:81553/1‑121 (MQ=255)

                                                                                   caGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAATGCTCTTGTTCCGACGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAg                                                                  >  2:366876/1‑149 (MQ=255)

                                                                                    aGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCt                                                                                     >  8:163838/1‑129 (MQ=255)

                                                                                    aGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCt                                                                                     <  7:163838/129‑1 (MQ=255)

                                                                                     gggATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCCATCCTTCTGCCGTTTGCTGGCCTGCAGcc                                                                <  2:29178/149‑1 (MQ=255)

                                                                                       gATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGt                                                              <  4:182046/149‑1 (MQ=255)

                                                                                       gATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGt                                                              >  7:66708/1‑149 (MQ=255)

                                                                                         tCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTg                                                            >  2:105338/1‑149 (MQ=255)

                                                                                         tCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTg                                                            >  7:193968/1‑149 (MQ=255)

                                                                                           aaGCCTAACTCAGCCCTGACGGATCATCCACCCCCTCAACCGGCGGAACAATTGATATTGTTACGCCGCTTTGTTTGCTGGCAAGGCAATCAATGACAAAA‑GTCGCTACTCGCTATCCTTCTGCCGTTTGATCGACTTCAGccttagc                                                           >  2:266018/1‑143 (MQ=255)

                                                                                           aaGACTGACTCAGCGCTGAGGTCTCATCCACCCCCGCACCCTGCGGAACAATTGCTCTTGTTCCGCCGCTTTTTTTGCTGGCATTGGAATCAATGACAATT‑GTGGTTACTTGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGc                                                           <  1:266018/148‑1 (MQ=255)

                                                                                              ccTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTggg                                                       <  1:105338/149‑1 (MQ=255)

                                                                                                    tCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGt                                                 >  6:95181/1‑149 (MQ=255)

                                                                                                      aGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑g                                              <  5:262834/149‑1 (MQ=255)

                                                                                                      aGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGACTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑g                                              >  2:109148/1‑149 (MQ=255)

                                                                                                         ccTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCgg                                           >  3:7545/1‑149 (MQ=255)

                                                                                                                 cTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCt                                   >  1:140209/1‑149 (MQ=255)

                                                                                                                  tCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑Gc                                             >  1:20469/1‑138 (MQ=255)

                                                                                                                  tCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑Gc                                             <  2:20469/138‑1 (MQ=255)

                                                                                                                  tCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTg                                  >  1:367891/1‑149 (MQ=255)

                                                                                                                  tCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTg                                  >  2:215187/1‑149 (MQ=255)

                                                                                                                     tCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGt                                                 >  8:278416/1‑132 (MQ=255)

                                                                                                                     tCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGt                                                 <  7:278416/132‑1 (MQ=255)

                                                                                                                     tCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTATGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCgg                               >  1:286242/1‑149 (MQ=255)

                                                                                                                        aCCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTg                            <  5:155474/149‑1 (MQ=255)

                                                                                                                        aCCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTg                            >  4:335920/1‑149 (MQ=255)

                                                                                                                        aCCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTg                            >  2:57511/1‑149 (MQ=255)

                                                                                                                         cccccGCACGCGGCGGAACAATTGCTCTNGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTgg                           <  4:62451/149‑1 (MQ=255)

                                                                                                                          ccccGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGc                          <  6:344723/149‑1 (MQ=255)

                                                                                                                          ccccGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGc                          <  5:95181/149‑1 (MQ=255)

                                                                                                                           cccGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCg                         >  6:218413/1‑149 (MQ=255)

                                                                                                                           cccGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCg                         <  4:261376/149‑1 (MQ=255)

                                                                                                                           cccGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCg                         >  4:266057/1‑149 (MQ=255)

                                                                                                                             cGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCgta                       <  6:95750/149‑1 (MQ=255)

                                                                                                                             cGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCgta                       <  6:101271/149‑1 (MQ=255)

                                                                                                                              gCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCgtag                      <  3:104895/149‑1 (MQ=255)

                                                                                                                               cACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGc                                 <  6:144303/137‑1 (MQ=255)

                                                                                                                               cACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCgtag                      >  4:143499/1‑148 (MQ=255)

                                                                                                                                aCCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCgtagta                    <  1:57511/149‑1 (MQ=255)

                                                                                                                                 cccGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTTCGCGGTCAGCGCTGCGGGTGGCgtagta                    <  1:215187/149‑1 (MQ=255)

                                                                                                                                   cggcggAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGat                 <  5:296136/149‑1 (MQ=255)

                                                                                                                                    ggcggAACAAGAGCAATTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGata                >  1:313375/1‑149 (MQ=255)

                                                                                                                                     gcggAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCACTGCGGGTGGCGTAGTAGatat               <  8:193968/149‑1 (MQ=255)

                                                                                                                                     gcggAACAAGAGCAATTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGGTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGata                >  1:111593/1‑149 (MQ=255)

                                                                                                                                        gAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCgg            <  4:23976/149‑1 (MQ=255)

                                                                                                                                         aaCAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGc           <  5:135612/149‑1 (MQ=255)

                                                                                                                                            aaTTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGt        >  7:5763/1‑149 (MQ=255)

                                                                                                                                            aaTTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGt        >  5:68389/1‑149 (MQ=255)

                                                                                                                                                gCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTggg     >  1:48329/1‑148 (MQ=255)

                                                                                                                                                  tctTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTAc  >  8:80969/1‑149 (MQ=255)

CGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAC‑GCCCCGCCACAGGGATCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATT‑GTCGGTACTGGCTATCCTTCTGCCGTTTGCTGGCCTGCAGCCGTTGCTGGGCTGCGTT‑GCGGTCAGCGCTGCGGGTGGCGTAGTAGATATCGGCCGTGGGTAC  >  NC_002947/494936‑495229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`