Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	532,945	C→G	6.5%	Y70* (TAC→TAG)	tufA →	elongation factor Tu‑A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	532,945	0	C	G	6.5%	178.4 / 3.4	61	Y70* (TAC→TAG)	tufA	elongation factor Tu‑A
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base G (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

ACCACGGTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGA  >  NC_002947/532800‑533088                                                                                                                                                  |                                                                                                                                                aCCACGGTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACaa                                                                                                                                               >  3:335049/1‑148 (MQ=34) aCCACGGTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACaa                                                                                                                                               >  8:201145/1‑148 (MQ=34)   cACGGTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTc                                                                                                                                            >  1:164251/1‑149 (MQ=34)     cgttAAGTCCTCTCTGACCGCAGCTCTGACTCGCGTTTGCTCCTAAGTTTTCGGTTCGGCAATCGTTTAGTTCGACAATATCGACTCGGCTCCGGAAGAAAAAGCTCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGa                                                                                                                                          <  2:243277/146‑1 (MQ=11)       gTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGCAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTTGTACTCGAcg                                                                                                                                        <  8:364/149‑2 (MQ=255)       gTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGAcc                                                                                                                                        <  4:335049/149‑1 (MQ=35)              cACTCTGACCGCAGCTCTGACTCTCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTc                                                                                                                                 <  2:176518/149‑1 (MQ=37)              cACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGt                                                                                                                                  >  7:273072/1‑148 (MQ=37)              cACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGt                                                                                                                                  >  5:173578/1‑148 (MQ=37)               aCTCTGACCGCAGCTCTGAGTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCa                                                                                                                                <  4:284742/149‑1 (MQ=37)               aCTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCa                                                                                                                                <  3:267537/149‑1 (MQ=37)                     aCCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGCCCATTCGTCACTACGc                                                                                                                          >  8:245100/1‑149 (MQ=37)                       cGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCt                                                                                                                         >  7:198898/1‑148 (MQ=37)                            ctctGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGAGCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGtt                                                                                                                   <  6:290966/149‑1 (MQ=37)                                 aCTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACt                                                                                                               <  2:367417/148‑1 (MQ=37)                                   tCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCAGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGcc                                                                                                            >  2:199760/1‑149 (MQ=37)                                     gcgTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTTGTACTCGAcgtga                                                                                                                                     <  2:185874/122‑5 (MQ=11)                                     gcgTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTTGTACTCGAcgtga                                                                                                                                     >  1:185874/1‑118 (MQ=11)                                      cgTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAg                                                                                                         <  1:271953/149‑1 (MQ=37)                                          tGCTCCGTAGTTTTCGGTTCGGGATTGGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAATAAGCGCGCTGTATCACCATCAACTCCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCTGGTc                                                                                                      <  7:320060/148‑1 (MQ=11)                                             tCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCAc                                                                                                    <  3:290291/147‑1 (MQ=34)                                             tCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCAc                                                                                                    >  4:290291/1‑147 (MQ=34)                                             tCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGc                                                                                                  >  7:47706/1‑149 (MQ=34)                                               cGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCt                                                                                                 <  1:97460/148‑1 (MQ=34)                                                   gTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTa                                                                                            >  7:224503/1‑149 (MQ=34)                                                       tcggttcggCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCCGACTACGt                                                                                         <  7:201145/148‑1 (MQ=21)                                                         ggttcggCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTgaa                                                                                      <  8:224503/149‑1 (MQ=34)                                                          tttctgCAATAGTTGAGTTAGACAAGATCGACTCGGCTCAGGAAGAAAAAGAGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTAGACAATTCGTCACTACGCTAAAGTTGACTGACAAGGTCACGCTGNCTACTTg                                                                                        >  2:163252/2‑146 (MQ=11)                                                          gttcggCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTgaag                                                                                     <  2:164251/149‑1 (MQ=34)                                                              ggCAATCGTTGAGTTAGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACa                                                                                 <  6:284795/149‑1 (MQ=21)                                                                 aaTCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGa                                                                              <  3:22221/149‑1 (MQ=33)                                                                  aTCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTAc                                                                                           <  3:46888/135‑1 (MQ=33)                                                                  aTCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTAc                                                                                           >  4:46888/1‑135 (MQ=33)                                                                   tCGTTGAGTTCGACAAGATCGACTCGGATCCTGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATc                                                                            <  1:163252/149‑1 (MQ=17)                                                                     gTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCAc                                                                          <  6:173578/149‑1 (MQ=32)                                                                      ttGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCAcc                                                                         >  4:166670/1‑149 (MQ=32)                                                                          gTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCgggg                                                                     <  7:245100/149‑3 (MQ=17)                                                                           ttCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGtgc                                                                    >  4:343547/1‑149 (MQ=32)                                                                                  aGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGcc                                                                >  3:227255/1‑146 (MQ=32)                                                                                         cTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACg                                                      <  8:47706/149‑1 (MQ=32)                                                                                               tCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGa                                                >  5:115867/1‑149 (MQ=32)                                                                                                   gaagaaAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCCCGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCt                                            >  8:278330/1‑149 (MQ=17)                                                                                                         aaaGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTg                                      >  6:54436/1‑149 (MQ=32)                                                                                                         aaaGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTg                                      >  3:246573/1‑149 (MQ=32)                                                                                                         aaaGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTg                                      >  5:50203/1‑149 (MQ=32)                                                                                                                 cgGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGgccg                              <  8:198898/149‑1 (MQ=32)                                                                                                                      tCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGAt                         >  3:343735/1‑149 (MQ=32)                                                                                                                       cACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTc                                   <  1:16397/138‑1 (MQ=32)                                                                                                                       cACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTc                                   >  2:16397/1‑138 (MQ=32)                                                                                                                              aaCACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGAt                 <  3:343547/149‑1 (MQ=32)                                                                                                                              aaCACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGAt                 <  1:199760/149‑1 (MQ=32)                                                                                                                              aaCACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGAt                 >  3:269248/1‑149 (MQ=32)                                                                                                                              aaCACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGAt                 <  4:227255/149‑1 (MQ=32)                                                                                                                                cacCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGc               >  7:118323/1‑149 (MQ=32)                                                                                                                                 acCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCGGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGc               >  6:344909/1‑148 (MQ=17)                                                                                                                                   cGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCca             >  8:350926/1‑148 (MQ=32)                                                                                                                                     gaatggTCGAGTTGTACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCcaca           >  5:160009/6‑148 (MQ=255)                                                                                                                                          gTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCg     >  4:190292/1‑149 (MQ=34)                                                                                                                                             gAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGt    >  5:195672/1‑147 (MQ=34)                                                                                                                                             gAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGa  <  3:190292/149‑1 (MQ=34)                                                                                                                                             gAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGa  <  3:166670/149‑1 (MQ=34)                                                                                                                                                  |                                                                                                                                                ACCACGGTAAGACCACTCTGACCGCAGCTCTGACTCGCGTTTGCTCCGAAGTTTTCGGTTCGGCAATCGTTGAGTTCGACAAGATCGACTCGGCTCCGGAAGAAAAAGCGCGCGGTATCACCATCAACACCGCTCACGTCGAGTACAACTCGACCATTCGTCACTACGCTCACGTTGACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCCGCCGATGGTCCGATGCCACAAACCCGTGA  >  NC_002947/532800‑533088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑