Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 535,818 G→T 6.1% E85* (GAA→TAA) ‡ rplA → 50S ribosomal protein L1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947535,8180GT 6.1% 242.7 / 3.1 75E85* (GAA→TAA) ‡rplA50S ribosomal protein L1
Reads supporting (aligned to +/- strand):  ref base G (36/34);  new base T (2/3);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTCCTCG  >  NC_002947/535672‑535966
                                                                                                                                                  |                                                                                                                                                    
ccAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTg                                                                                                                                                      >  6:61851/1‑147 (MQ=255)
        gtcgAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGTGTCCAGCAGCTTCAgctgctg                                                                                                                                             <  4:274535/148‑2 (MQ=255)
         tcgAGTCCTACGACATCTCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACGAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTTCAgctgctgg                                                                                                                                            <  5:263782/148‑3 (MQ=255)
             gTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGCCCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTg                                                                                                                                       >  2:254451/1‑149 (MQ=255)
              tCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGc                                                                                                                                      <  5:392524/149‑1 (MQ=255)
              tCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGc                                                                                                                                      >  8:45118/1‑149 (MQ=255)
                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTTCAgctgc                                                                                                                                               >  4:99776/1‑137 (MQ=255)
                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTTCAgctgc                                                                                                                                               <  3:99776/137‑1 (MQ=255)
                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCgg                                                                                                                                   >  4:48835/1‑149 (MQ=255)
                 tACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCgg                                                                                                                                   >  8:274267/1‑149 (MQ=255)
                    gACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGgcgc                                                                                                                                >  7:4153/1‑149 (MQ=255)
                       aTCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACAAGGTCGTACGTAGCGCTACCGAGCTGCCCCACGGCACTGGCAAGAACGTTCGCGTTGCTGTCTTCACCCAGGCTCCAGCAGCTGAATCTGCTCTGGCTGCGGCCGCTGa                                                                                                                             >  6:80181/1‑149 (MQ=255)
                        tCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGAt                                                                                                                            >  1:381153/1‑149 (MQ=255)
                           ccGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATcgc                                                                                                                         >  7:55321/1‑149 (MQ=255)
                            cGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATcgcg                                                                                                                        >  2:215000/1‑149 (MQ=255)
                              ttAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTa                                                                                                                      <  3:89300/149‑1 (MQ=255)
                              ttAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTa                                                                                                                      <  5:363781/149‑1 (MQ=255)
                                  ccTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTa                                                                                                                  <  5:278194/149‑1 (MQ=255)
                                   cTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTAt                                                                                                                 >  6:140664/1‑149 (MQ=255)
                                      ggTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATg                                                                                                                <  3:48835/147‑1 (MQ=255)
                                        tgtTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGAc                                                                                                             >  6:3530/1‑148 (MQ=255)
                                            gACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                         <  7:45118/148‑1 (MQ=255)
                                            gACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATc                                                                                                         <  3:366524/148‑1 (MQ=255)
                                            gACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCt                                                                                                        >  5:301657/1‑149 (MQ=255)
                                             aCCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTg                                                                                                       <  1:103051/149‑1 (MQ=255)
                                              cccGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGa                                                                                                              >  7:148802/1‑141 (MQ=255)
                                              cccGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGa                                                                                                              <  8:148802/141‑1 (MQ=255)
                                              cccGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGACCTgg                                                                                                      <  5:3530/149‑1 (MQ=255)
                                               ccGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGc                                                                                                     >  6:162356/1‑149 (MQ=255)
                                                 gcgTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATcgcg                                                                                                                        >  3:85007/1‑128 (MQ=255)
                                                 gcgTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATcgcg                                                                                                                        <  4:85007/128‑1 (MQ=255)
                                                   gTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCTAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGcc                                                                                                 <  2:313326/149‑1 (MQ=255)
                                                       aTCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCTGTGTCGAGGAGCTGAAGGTGCTCTGGCTGCCGGTGCTGATCGCGTATGTATTGACTATCTGGCTGCCGaaa                                                                                             <  8:14303/149‑1 (MQ=255)
                                                           gACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGaa                                                                                         <  1:200115/149‑1 (MQ=255)
                                                           gACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGa                                                                                          >  4:35087/1‑148 (MQ=255)
                                                              cAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAgg                                                                                      >  1:70886/1‑149 (MQ=255)
                                                                 gTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAggcgg                                                                                   <  5:162356/149‑1 (MQ=255)
                                                                    gtacgtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGgcggcg                                                                                 <  1:254451/148‑1 (MQ=255)
                                                                        gtaGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTg                                                                            <  8:55321/149‑1 (MQ=255)
                                                                           gcgcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGaa                                                                          <  7:217322/148‑1 (MQ=255)
                                                                            cgcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACt                                                                        <  6:19638/149‑1 (MQ=255)
                                                                             gcTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAATGCGGCGACCTGAACTa                                                                       >  6:287115/1‑149 (MQ=255)
                                                                              cTACCGTGCTGCCACACGGCACTGGCAAGACCTTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTATGTATGGACGATCTGGCTGTCGAAATGAAAGGCGGCGACCTGAACTAt                                                                      <  8:58039/149‑1 (MQ=255)
                                                                                aCCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGaaa                                                                                        >  4:333180/1‑129 (MQ=255)
                                                                                aCCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGaaa                                                                                        <  3:333180/129‑1 (MQ=255)
                                                                                 ccGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATg                                                                     <  5:140664/147‑1 (MQ=255)
                                                                                    tgctgcCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGAcgtc                                                                <  2:355923/149‑1 (MQ=255)
                                                                                       tgcCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGAcgtcgt                                                              >  1:347997/1‑148 (MQ=255)
                                                                                         ccACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTat                                                           >  4:22881/1‑149 (MQ=255)
                                                                                           acacGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcg                                                         >  4:81115/1‑149 (MQ=255)
                                                                                           acacGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcg                                                         >  2:42862/1‑149 (MQ=255)
                                                                                           acacGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcg                                                         <  7:274267/149‑1 (MQ=255)
                                                                                            cacGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTatcgc                                                        <  5:61851/149‑1 (MQ=255)
                                                                                                          gACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCa                                          >  6:43058/1‑149 (MQ=255)
                                                                                                                    gTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGt                                <  2:70886/149‑1 (MQ=255)
                                                                                                                      tGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAg                              <  6:301657/149‑1 (MQ=255)
                                                                                                                       gCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAgg                             >  7:18022/1‑149 (MQ=255)
                                                                                                                        cTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGt                            <  1:42862/149‑1 (MQ=255)
                                                                                                                        cTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGt                            >  8:224294/1‑149 (MQ=255)
                                                                                                                         tGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGt                            >  8:218473/1‑148 (MQ=255)
                                                                                                                           tCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAg                         >  5:195365/1‑149 (MQ=255)
                                                                                                                             ttCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCt                       >  8:47506/1‑149 (MQ=255)
                                                                                                                              tCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTg                      >  2:36132/1‑149 (MQ=255)
                                                                                                                                  ccAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGt                   >  3:93507/1‑148 (MQ=255)
                                                                                                                                  ccAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTc                  <  3:81115/149‑1 (MQ=255)
                                                                                                                                   cAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGt                   >  2:359982/1‑147 (MQ=255)
                                                                                                                                   cAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGt                   >  4:16931/1‑147 (MQ=255)
                                                                                                                                    aGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGAGCGTTGTAGGTAAGCTGGGTc                  >  6:269683/1‑147 (MQ=255)
                                                                                                                                     gggTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAgg               <  1:36132/149‑1 (MQ=255)
                                                                                                                                        tCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAg                              <  1:278931/131‑1 (MQ=255)
                                                                                                                                        tCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAg                              >  2:278931/1‑131 (MQ=255)
                                                                                                                                         ccAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCt           <  2:381153/149‑1 (MQ=255)
                                                                                                                                         agagcagcTTCAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCt           >  2:42021/3‑149 (MQ=255)
                                                                                                                                           agcagcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTgg         <  8:4153/149‑1 (MQ=255)
                                                                                                                                           agcagcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCCTTGTAGGTCAGCTGGGTCAGGTTCTgg         <  1:215000/149‑1 (MQ=255)
                                                                                                                                               gcTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTcc     >  5:147690/1‑149 (MQ=255)
                                                                                                                                                  gAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTCCTcg  >  1:40170/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCAAGTTCGTCGAGTCCTACGACATCGCCGTTAACCTCGGTGTTGACCCGCGTAAATCCGACCAGGTCGTACGTAGCGCTACCGTGCTGCCACACGGCACTGGCAAGACCGTTCGCGTTGCTGTCTTCACCCAGGGTCCAGCAGCTGAAGCTGCTCTGGCTGCCGGCGCTGATCGCGTAGGTATGGACGATCTGGCTGCCGAAATGAAAGGCGGCGACCTGAACTATGACGTCGTTATCGCATCGCCTGATGCCATGCGCGTTGTAGGTCAGCTGGGTCAGGTTCTGGGTCCTCG  >  NC_002947/535672‑535966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑