Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 563,006 C→G 6.7% P30R (CCT→CGT) ‡ rpoA → DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947563,0060CG 6.7% 326.0 / 9.5 101P30R (CCT→CGT) ‡rpoADNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (45/49);  new base G (2/5);  total (47/54)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TCAACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAA  >  NC_002947/562858‑563152
                                                                                                                                                    |                                                                                                                                                  
tCAACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGcc                                                                                                                                                    >  2:362215/1‑149 (MQ=255)
   aCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCctct                                                                                                                                                 >  1:194083/1‑149 (MQ=255)
     gAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCtctc                                                                                                                                                >  3:253544/1‑148 (MQ=255)
      aaaaCCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGa                                                                                                                                              <  5:294410/149‑1 (MQ=255)
          ccTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGt                                                                                                                                          <  6:7489/149‑1 (MQ=255)
          ccTGATTGGCCATCTCTACTCCTAGTAATGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAATTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGAAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGt                                                                                                                                          <  2:330889/149‑1 (MQ=255)
           cTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                         <  3:154823/149‑1 (MQ=255)
           cTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGt                                                                                                                                          >  8:295088/1‑148 (MQ=255)
             gATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGt                                                                                                                                       >  3:254671/1‑149 (MQ=255)
              aTTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGtaa                                                                                                                                        <  7:295088/147‑3 (MQ=255)
               ttGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGtt                                                                                                                                      <  1:362215/148‑1 (MQ=255)
               ttGTAGAGCTCAACTCCAAGTAAGCGCTAGAAAATAGGTGGATCCATGCAGATGTCCGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGtaattt                                                                                                                                     <  7:302778/149‑6 (MQ=255)
                  tCGAGCTCTACTCCAAGTAAGGGCTAGACAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTCGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                   <  7:278833/148‑1 (MQ=255)
                  tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAACTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCC‑AAATTACGCTCGAGCCTCTCGAGCGTGGTTTCgg                                                                                                                                  <  8:357112/148‑1 (MQ=255)
                  tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCATAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                   <  1:47969/148‑1 (MQ=255)
                    gAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCTCATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                         >  1:123540/1‑140 (MQ=255)
                    gAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                         <  2:123540/140‑1 (MQ=255)
                       ctctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcgg                                                                                                                             <  6:277085/149‑5 (MQ=37)
                       ctctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcgg                                                                                                                             <  2:69151/149‑5 (MQ=37)
                        tctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAc                                                                                                                            >  3:351847/1‑149 (MQ=255)
                        tctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAc                                                                                                                            >  8:121232/1‑149 (MQ=255)
                         ctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAcc                                                                                                                           >  7:348332/1‑149 (MQ=255)
                               cAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTggg                                                                                                                      >  6:153224/1‑148 (MQ=255)
                                aaGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGc                                                                                                                     >  1:177621/1‑148 (MQ=255)
                                aaGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGc                                                                                                                     >  2:66404/1‑148 (MQ=255)
                                  gTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAAc                                                                                                                  >  6:340307/1‑149 (MQ=255)
                                        gCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTg                                                                                                            >  7:209843/1‑149 (MQ=255)
                                        gCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTg                                                                                                            >  7:148788/1‑149 (MQ=255)
                                        gCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTg                                                                                                            <  2:247547/149‑1 (MQ=255)
                                                 tAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATc                                                                                                   <  8:348332/149‑1 (MQ=255)
                                                    gTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtg                                                                                                >  4:166894/1‑149 (MQ=255)
                                                      gCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                              >  1:45768/1‑149 (MQ=255)
                                                       cATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                              <  4:253544/148‑1 (MQ=255)
                                                       cATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                              <  2:231412/148‑1 (MQ=255)
                                                          ccATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTgg                                                                                                                       >  3:273843/1‑120 (MQ=255)
                                                            aTGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctc                                                                                        >  2:388542/1‑149 (MQ=255)
                                                                 gATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGc                                                                                   >  5:321598/1‑149 (MQ=255)
                                                                       ggTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCt                                                                             <  4:315008/149‑1 (MQ=255)
                                                                       ggTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCt                                                                             >  2:19805/1‑149 (MQ=255)
                                                                         tAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCTGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCGTGGCtgt                                                                           <  6:124777/149‑1 (MQ=255)
                                                                         tAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgt                                                                           >  8:243355/1‑149 (MQ=255)
                                                                           aaTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                          <  1:19805/148‑1 (MQ=255)
                                                                            aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAATGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                          >  4:303112/1‑147 (MQ=255)
                                                                            aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                        <  7:121232/149‑1 (MQ=255)
                                                                             tGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                          >  2:147505/1‑146 (MQ=255)
                                                                             tGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCag                                                                       <  6:321598/149‑1 (MQ=255)
                                                                             tGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                        <  1:147505/148‑1 (MQ=255)
                                                                               aGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCagta                                                                     <  1:66404/149‑1 (MQ=255)
                                                                                   ccTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                                  >  4:183463/1‑148 (MQ=255)
                                                                                   ccTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                                  >  1:196275/1‑148 (MQ=255)
                                                                                    cTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAACGTCGTTTCGGCAAAACCCTGGGCAACGCTCTGCGCCGCAAACAGTTGTCCTCCATGCCTCGCTGTGCAGTAGTCGa                                                                >  6:344806/1‑149 (MQ=255)
                                                                                     tGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAg                                                               >  2:252046/1‑149 (MQ=255)
                                                                                       aCTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGc                                                             <  4:100639/149‑1 (MQ=255)
                                                                                       aCTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGc                                                             >  6:166273/1‑149 (MQ=255)
                                                                                       aCTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGc                                                             >  8:202350/1‑149 (MQ=255)
                                                                                         tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                            >  2:62335/1‑148 (MQ=255)
                                                                                           cccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                            >  3:281542/1‑146 (MQ=255)
                                                                                            gggCCACATTGATGTTCAGGTAGTCAGTCCGAGCCGCGCCAAATTTACGCTCGAGCGTCTCGAGCGTGGTTTCGGCCTTACCCTGGGGAAGGGGCTGCGCCGCATCGTGTTGTCCTCGATGGCTGGCTGTGCAGTAGTCGAGGCCgaga                                                        <  5:344806/147‑1 (MQ=255)
                                                                                             cGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                            >  6:36695/1‑144 (MQ=255)
                                                                                                  caTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATg                                                  <  4:254671/149‑1 (MQ=255)
                                                                                                  caTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATg                                                  <  7:67328/149‑1 (MQ=255)
                                                                                                    ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGc                                                                              >  1:144749/1‑119 (MQ=255)
                                                                                                    ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGc                                                                              <  2:144749/119‑1 (MQ=255)
                                                                                                    ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                                <  5:340307/149‑1 (MQ=255)
                                                                                                    ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                                <  8:209843/149‑1 (MQ=255)
                                                                                                         gtgCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGc                                                                         <  4:313165/119‑1 (MQ=255)
                                                                                                         gtgCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGc                                                                         >  3:313165/1‑119 (MQ=255)
                                                                                                         gtgCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGACGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACt                                           >  7:207401/1‑149 (MQ=255)
                                                                                                           gCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCGGGCTGTGCAGTAGTCGAGGCCGAGATCGAAGGCGTACTc                                          >  4:64801/1‑148 (MQ=255)
                                                                                                            cAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCa                                        <  2:196275/149‑1 (MQ=255)
                                                                                                               gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCGATGa                                     >  4:56154/1‑149 (MQ=255)
                                                                                                               gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCAt                                       >  7:136445/1‑147 (MQ=255)
                                                                                                               gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                     <  4:281542/149‑1 (MQ=255)
                                                                                                               gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                     <  5:153224/149‑1 (MQ=255)
                                                                                                               gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                     <  3:183463/149‑1 (MQ=255)
                                                                                                               gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCGGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                     <  6:326656/149‑1 (MQ=255)
                                                                                                                   tcagtcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTAc                                 >  8:54327/1‑149 (MQ=255)
                                                                                                                      gtcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCATGGAGTACGcc                              <  1:62335/149‑1 (MQ=255)
                                                                                                                         cGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCcgcg                           <  7:202350/149‑1 (MQ=255)
                                                                                                                          gACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGa                          <  2:49923/149‑1 (MQ=255)
                                                                                                                           aCCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGAt                         >  8:158342/1‑149 (MQ=255)
                                                                                                                             ccGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGACGGCGTACTCCATGAGTACTCCGCGATCg                       <  3:64801/149‑1 (MQ=255)
                                                                                                                               gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                     <  8:148788/149‑1 (MQ=255)
                                                                                                                                ctccAAAATTACGCTCGAGCCTCTCGAGCGGGGTTTCTGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTTGTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                    <  3:268339/147‑1 (MQ=255)
                                                                                                                                   cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGgtg                 >  1:78536/1‑149 (MQ=255)
                                                                                                                                   cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTACGCGAGCGAAGgtg                 >  8:244847/1‑149 (MQ=255)
                                                                                                                                     aaaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGtt               <  8:207401/149‑1 (MQ=255)
                                                                                                                                     aaaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGtt               <  3:82406/149‑1 (MQ=255)
                                                                                                                                      aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                              >  1:129496/1‑69 (MQ=255)
                                                                                                                                      aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                              <  2:129496/69‑1 (MQ=255)
                                                                                                                                      aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTc              <  7:243355/149‑1 (MQ=255)
                                                                                                                                        ttACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                    <  5:302679/141‑1 (MQ=255)
                                                                                                                                        ccaCGCTCGAGAGGCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCAGCGATCGAAGGTGTTCAg            >  8:311684/3‑149 (MQ=255)
                                                                                                                                           cGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGaa         <  1:252046/149‑1 (MQ=255)
                                                                                                                                            gCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCGTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAg        >  6:376366/1‑149 (MQ=255)
                                                                                                                                              tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGa                                                                >  4:236687/1‑91 (MQ=255)
                                                                                                                                                gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc      <  2:194083/147‑1 (MQ=255)
                                                                                                                                                gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc      <  5:166273/147‑1 (MQ=255)
                                                                                                                                                gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc      <  5:36695/147‑1 (MQ=255)
                                                                                                                                                  gCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTaa  <  8:136445/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCAACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAA  >  NC_002947/562858‑563152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑