Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 563,007 T→G 5.9% P30P (CCT→CCG) ‡ rpoA → DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947563,0070TG 5.9% 315.4 / 6.4 100P30P (CCT→CCG) ‡rpoADNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (44/50);  new base G (2/4);  total (46/54)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 105 total aligned reads displayed.

ACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAATT  >  NC_002947/562861‑563154
                                                                                                                                                  |                                                                                                                                                   
aCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCctct                                                                                                                                                   >  1:194083/1‑149 (MQ=255)
  gAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCtctc                                                                                                                                                  >  3:253544/1‑148 (MQ=255)
   aaaaCCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGa                                                                                                                                                <  5:294410/149‑1 (MQ=255)
       ccTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGt                                                                                                                                            <  6:7489/149‑1 (MQ=255)
       ccTGATTGGCCATCTCTACTCCTAGTAATGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAATTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGAAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGt                                                                                                                                            <  2:330889/149‑1 (MQ=255)
        cTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                           <  3:154823/149‑1 (MQ=255)
        cTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGt                                                                                                                                            >  8:295088/1‑148 (MQ=255)
          gATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGt                                                                                                                                         >  3:254671/1‑149 (MQ=255)
           aTTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGtaa                                                                                                                                          <  7:295088/147‑3 (MQ=255)
            ttGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGtt                                                                                                                                        <  1:362215/148‑1 (MQ=255)
            ttGTAGAGCTCAACTCCAAGTAAGCGCTAGAAAATAGGTGGATCCATGCAGATGTCCGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGtaattt                                                                                                                                       <  7:302778/149‑6 (MQ=255)
               tCGAGCTCTACTCCAAGTAAGGGCTAGACAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTCGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                     <  7:278833/148‑1 (MQ=255)
               tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAACTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCC‑AAATTACGCTCGAGCCTCTCGAGCGTGGTTTCgg                                                                                                                                    <  8:357112/148‑1 (MQ=255)
               tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCATAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                     <  1:47969/148‑1 (MQ=255)
                 gAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCTCATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                           >  1:123540/1‑140 (MQ=255)
                 gAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTg                                                                                                                                           <  2:123540/140‑1 (MQ=255)
                    ctctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcgg                                                                                                                               <  6:277085/149‑5 (MQ=37)
                    ctctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgcgg                                                                                                                               <  2:69151/149‑5 (MQ=37)
                     tctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAc                                                                                                                              >  3:351847/1‑149 (MQ=255)
                     tctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAc                                                                                                                              >  8:121232/1‑149 (MQ=255)
                      ctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAcc                                                                                                                             >  7:348332/1‑149 (MQ=255)
                            cAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTggg                                                                                                                        >  6:153224/1‑148 (MQ=255)
                             aaGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGc                                                                                                                       >  1:177621/1‑148 (MQ=255)
                             aaGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGc                                                                                                                       >  2:66404/1‑148 (MQ=255)
                               gTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAAc                                                                                                                    >  6:340307/1‑149 (MQ=255)
                                     gCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTg                                                                                                              >  7:148788/1‑149 (MQ=255)
                                     gCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTg                                                                                                              <  2:247547/149‑1 (MQ=255)
                                              tAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATc                                                                                                     <  8:348332/149‑1 (MQ=255)
                                                 gTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtg                                                                                                  >  4:166894/1‑149 (MQ=255)
                                                   gCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                >  1:45768/1‑149 (MQ=255)
                                                    cATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                <  4:253544/148‑1 (MQ=255)
                                                    cATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                <  2:231412/148‑1 (MQ=255)
                                                       ccATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTgg                                                                                                                         >  3:273843/1‑120 (MQ=255)
                                                         aTGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctc                                                                                          >  2:388542/1‑149 (MQ=255)
                                                                    ggTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCt                                                                               >  2:19805/1‑149 (MQ=255)
                                                                    ggTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCt                                                                               <  4:315008/149‑1 (MQ=255)
                                                                      tAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCTGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCGTGGCtgt                                                                             <  6:124777/149‑1 (MQ=255)
                                                                      tAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgt                                                                             >  8:243355/1‑149 (MQ=255)
                                                                        aaTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                            <  1:19805/148‑1 (MQ=255)
                                                                         aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAATGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                            >  4:303112/1‑147 (MQ=255)
                                                                         aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                          <  7:121232/149‑1 (MQ=255)
                                                                          tGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                            >  2:147505/1‑146 (MQ=255)
                                                                          tGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCag                                                                         <  6:321598/149‑1 (MQ=255)
                                                                          tGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                          <  1:147505/148‑1 (MQ=255)
                                                                            aGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCagta                                                                       <  1:66404/149‑1 (MQ=255)
                                                                                ccTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                                    >  1:196275/1‑148 (MQ=255)
                                                                                ccTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                                    >  4:183463/1‑148 (MQ=255)
                                                                                 cTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAACGTCGTTTCGGCAAAACCCTGGGCAACGCTCTGCGCCGCAAACAGTTGTCCTCCATGCCTCGCTGTGCAGTAGTCGa                                                                  >  6:344806/1‑149 (MQ=255)
                                                                                  tGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAg                                                                 >  2:252046/1‑149 (MQ=255)
                                                                                    aCTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGc                                                               >  8:202350/1‑149 (MQ=255)
                                                                                    aCTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGc                                                               >  6:166273/1‑149 (MQ=255)
                                                                                    aCTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGc                                                               <  4:100639/149‑1 (MQ=255)
                                                                                      tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                              >  2:62335/1‑148 (MQ=255)
                                                                                        cccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                              >  3:281542/1‑146 (MQ=255)
                                                                                         gggCCACATTGATGTTCAGGTAGTCAGTCCGAGCCGCGCCAAATTTACGCTCGAGCGTCTCGAGCGTGGTTTCGGCCTTACCCTGGGGAAGGGGCTGCGCCGCATCGTGTTGTCCTCGATGGCTGGCTGTGCAGTAGTCGAGGCCgaga                                                          <  5:344806/147‑1 (MQ=255)
                                                                                          cGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                              >  6:36695/1‑144 (MQ=255)
                                                                                               caTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATg                                                    <  7:67328/149‑1 (MQ=255)
                                                                                               caTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATg                                                    <  4:254671/149‑1 (MQ=255)
                                                                                                 ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGc                                                                                >  1:144749/1‑119 (MQ=255)
                                                                                                 ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGc                                                                                <  2:144749/119‑1 (MQ=255)
                                                                                                 ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                                  <  5:340307/149‑1 (MQ=255)
                                                                                                 ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                                  <  8:209843/149‑1 (MQ=255)
                                                                                                      gtgCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGc                                                                           <  4:313165/119‑1 (MQ=255)
                                                                                                      gtgCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGACGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACt                                             >  7:207401/1‑149 (MQ=255)
                                                                                                        gCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCGGGCTGTGCAGTAGTCGAGGCCGAGATCGAAGGCGTACTc                                            >  4:64801/1‑148 (MQ=255)
                                                                                                         cAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCa                                          <  2:196275/149‑1 (MQ=255)
                                                                                                            gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCGATGa                                       >  4:56154/1‑149 (MQ=255)
                                                                                                            gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCAt                                         >  7:136445/1‑147 (MQ=255)
                                                                                                            gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                       <  4:281542/149‑1 (MQ=255)
                                                                                                            gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                       <  5:153224/149‑1 (MQ=255)
                                                                                                            gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                       <  3:183463/149‑1 (MQ=255)
                                                                                                            gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCGGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                       <  6:326656/149‑1 (MQ=255)
                                                                                                                tcagtcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTAc                                   >  8:54327/1‑149 (MQ=255)
                                                                                                                   gtcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCATGGAGTACGcc                                <  1:62335/149‑1 (MQ=255)
                                                                                                                      cGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCcgcg                             <  7:202350/149‑1 (MQ=255)
                                                                                                                       gACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGa                            <  2:49923/149‑1 (MQ=255)
                                                                                                                        aCCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGAt                           >  8:158342/1‑149 (MQ=255)
                                                                                                                          ccGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGACGGCGTACTCCATGAGTACTCCGCGATCg                         <  3:64801/149‑1 (MQ=255)
                                                                                                                            gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                       <  8:148788/149‑1 (MQ=255)
                                                                                                                             ctccAAAATTACGCTCGAGCCTCTCGAGCGGGGTTTCTGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTTGTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                      <  3:268339/147‑1 (MQ=255)
                                                                                                                                cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGgtg                   >  1:78536/1‑149 (MQ=255)
                                                                                                                                cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTACGCGAGCGAAGgtg                   >  8:244847/1‑149 (MQ=255)
                                                                                                                                  aaaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGtt                 <  8:207401/149‑1 (MQ=255)
                                                                                                                                  aaaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGtt                 <  3:82406/149‑1 (MQ=255)
                                                                                                                                   aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                >  1:129496/1‑69 (MQ=255)
                                                                                                                                   aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgtt                                                                                                <  2:129496/69‑1 (MQ=255)
                                                                                                                                   aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTc                <  7:243355/149‑1 (MQ=255)
                                                                                                                                     ttACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                      >  6:302679/1‑141 (MQ=255)
                                                                                                                                     ttACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                      <  5:302679/141‑1 (MQ=255)
                                                                                                                                     ccaCGCTCGAGAGGCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCAGCGATCGAAGGTGTTCAg              >  8:311684/3‑149 (MQ=255)
                                                                                                                                        cGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGaa           <  1:252046/149‑1 (MQ=255)
                                                                                                                                         gCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCGTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAg          >  6:376366/1‑149 (MQ=255)
                                                                                                                                           tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGa                                                                  <  3:236687/91‑1 (MQ=255)
                                                                                                                                           tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGa                                                                  >  4:236687/1‑91 (MQ=255)
                                                                                                                                             gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc        <  5:166273/147‑1 (MQ=255)
                                                                                                                                             gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc        <  5:36695/147‑1 (MQ=255)
                                                                                                                                               gCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTaa    <  8:136445/149‑1 (MQ=255)
                                                                                                                                                  tctcGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAAtt  >  3:180255/1‑148 (MQ=255)
                                                                                                                                                  tctcGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAAtt  >  5:263058/1‑148 (MQ=255)
                                                                                                                                                  tctcGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAAtt  >  1:316932/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACGTAATT  >  NC_002947/562861‑563154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑