Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 585,116 T→C 7.7% Q47R (CAG→CGG) ‡ ansA ← type 1 L‑asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947585,1160TC 7.7% 182.8 / 5.1 65Q47R (CAG→CGG) ‡ansAtype 1 L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base T (26/34);  new base C (2/3);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

GTCGGTACCGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATGGAAAGCA  >  NC_002947/584971‑585263
                                                                                                                                                 |                                                                                                                                                   
gTCGGTACCGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTggg                                                                                                                                                  >  6:309887/1‑149 (MQ=255)
 tCGGTACCGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTggg                                                                                                                                                  <  3:255856/148‑1 (MQ=255)
 tCGGTACCGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTggg                                                                                                                                                  <  3:260817/148‑1 (MQ=255)
       ccGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCCCAGGTGCaat                                                                                                                                           <  4:73726/149‑3 (MQ=255)
        cgggcagcaCCAACACGCCATCGTGGCCCGCGACATCCACCCCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTg                                                                                                                                          <  8:208850/146‑1 (MQ=255)
        cGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCTAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTg                                                                                                                                          <  3:217488/149‑1 (MQ=255)
          tGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGc                                                                                                                                        <  4:294476/149‑1 (MQ=255)
            cagcaCCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCAATGCACCCCAGGTGCAATGGACa                                                                                                                                      <  3:3158/149‑1 (MQ=255)
                 ccAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTgg                                                                                                                                 <  7:163558/149‑1 (MQ=255)
                     cacGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCa                                                                                                                              >  2:314907/1‑148 (MQ=255)
                      acGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCaaa                                                                                                                            <  6:165562/149‑1 (MQ=255)
                      acGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCaaa                                                                                                                            <  2:246829/149‑1 (MQ=255)
                      acGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCaaa                                                                                                                            <  2:140847/149‑1 (MQ=255)
                      acGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGGCCATTGCACCTGGGGTGCATTGGCCATCTGGGCaaa                                                                                                                            <  5:97114/149‑1 (MQ=255)
                        gCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAgt                                                                                                                          <  5:309887/149‑1 (MQ=255)
                              gTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCa                                                                                                                     <  1:329973/148‑1 (MQ=255)
                              gTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCAc                                                                                                                    <  6:93907/149‑1 (MQ=255)
                                  cccGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCAt                                                                                                                >  8:253063/1‑149 (MQ=255)
                                   ccGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATc                                                                                                               >  2:163627/1‑149 (MQ=255)
                                   ccGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATc                                                                                                               >  5:368667/1‑149 (MQ=255)
                                     gcgACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCAATGCACCCCAGGTGCAATgga                                                                                                                                        >  3:381936/1‑121 (MQ=255)
                                     gcgACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCAATGCACCCCAGGTGCAATgga                                                                                                                                        <  4:381936/122‑2 (MQ=255)
                                        aCATCCCCCCCCGCGACGCTGGCATCGCGCATCGCCAGCCAGCTGTGCGGGTGCATATTGGCGCTGTCGAGCAGCGGGGTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGc                                                                                                          <  8:316969/149‑1 (MQ=255)
                                             cACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCaa                                                                                                     <  4:59077/149‑1 (MQ=255)
                                               ccgccgCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCATTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCaaaa                                                                                                   <  1:311701/149‑1 (MQ=255)
                                               ccgccgCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCaaaa                                                                                                   <  2:381682/149‑1 (MQ=255)
                                                      gacgaTGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCAccgg                                                                                            >  2:40167/1‑149 (MQ=255)
                                                       acgaTGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCAccggc                                                                                           >  5:4429/1‑149 (MQ=255)
                                                         gaTGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCAccggccg                                                                                         >  8:318889/1‑149 (MQ=255)
                                                             gcatcgcgcatcgcCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAATCACCGGCCGGTGc                                                                                     <  7:317382/149‑1 (MQ=255)
                                                              catcgcgcatcgcCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTg                                                                                                                                          <  1:271215/95‑1 (MQ=255)
                                                              catcgcgcatcgcCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTg                                                                                                                                          >  2:271215/1‑95 (MQ=255)
                                                                  gcgcatcgcCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAgg                                                                                 <  3:15759/148‑1 (MQ=255)
                                                                         gccagccaGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCg                                                                          <  3:230362/148‑1 (MQ=255)
                                                                          ccagccagTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCggg                                                                        >  7:128769/1‑149 (MQ=255)
                                                                              ccagTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGttt                                                                    >  7:237474/1‑149 (MQ=255)
                                                                                agTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAACCCACCGGCCGGTGCCAGGCCTTCCGGGGTTTc                                                                   >  8:250877/1‑148 (MQ=255)
                                                                                  ttGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAg                                                                <  7:220081/149‑1 (MQ=255)
                                                                                          ttGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGa                                                        >  7:46389/1‑149 (MQ=255)
                                                                                            gCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATg                                                      >  5:279860/1‑149 (MQ=255)
                                                                                            gCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATg                                                      >  6:242212/1‑149 (MQ=255)
                                                                                             cATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATgg                                                     >  8:205856/1‑149 (MQ=255)
                                                                                                    gcgcTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCAcc                                              <  1:40167/149‑1 (MQ=255)
                                                                                                      gcTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCgg                                            >  7:42517/1‑149 (MQ=255)
                                                                                                      gcTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCgg                                            <  7:253063/149‑1 (MQ=255)
                                                                                                         gTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGta                                          >  8:25752/1‑148 (MQ=255)
                                                                                                          tCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCgg                                            <  6:115817/145‑1 (MQ=255)
                                                                                                          tCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCgg                                            >  5:115817/1‑145 (MQ=255)
                                                                                                          tCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGtata                                        <  6:279860/149‑1 (MQ=255)
                                                                                                          tCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGtata                                        <  5:165091/149‑1 (MQ=255)
                                                                                                           cGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCTGATGGTGCCACCGGTATag                                       <  8:353308/149‑1 (MQ=255)
                                                                                                                agcGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTGGAGCATGCCGATGGTGCCACCGGTATAGAGAAc                                  >  6:227136/1‑149 (MQ=255)
                                                                                                                  cGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTAACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAAc                                  >  3:52019/1‑147 (MQ=255)
                                                                                                                                gCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAGAACCTCCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACt                  <  7:250877/149‑1 (MQ=255)
                                                                                                                                 cAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTg                 >  4:88791/1‑149 (MQ=255)
                                                                                                                                      tCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTgcgcg             <  7:318889/148‑1 (MQ=255)
                                                                                                                                       ccAATGCACCCCAGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACAACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGACACCGGTATAGAGCACGAGAAGATTCTTTACTGCGCGCa           >  2:296996/1‑149 (MQ=255)
                                                                                                                                          ttGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACt                  >  5:316237/1‑139 (MQ=255)
                                                                                                                                          ttGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACt                  <  6:316237/139‑1 (MQ=255)
                                                                                                                                          ttGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATg         >  2:293692/1‑148 (MQ=255)
                                                                                                                                          ttGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATg         >  6:33228/1‑148 (MQ=255)
                                                                                                                                             cACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATGGaaa     <  5:282897/149‑1 (MQ=255)
                                                                                                                                             cACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATGGaaa     <  1:174180/149‑1 (MQ=255)
                                                                                                                                               ccTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATGGAAAGc   <  8:46389/149‑1 (MQ=255)
                                                                                                                                                cTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATGGAAAGCa  >  3:180170/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTCGGTACCGTGCAGCACCAACACGCCATCGTGGCCCGCGACATCCACCGCCGCGACGATGGCATCGCGCATCGCCAGCCAGTTGTGCTGTTGCATATTGGCGCTGTCGAGCAGCGGGTTCAGCTCGTGCAGTGTCCATTGCACCTGGGGTGCATTGGCCATCTGGGCAAAGTGTTCACGCATCCGTGCTTCAAAACCACCGGCCGGTGCCAGGCCTTCCGGGGTTTCGAGCATGCCGATGGTGCCACCGGTATAGAGAACGAGAAGATTCTTTACTGCGCGCATGGAAAGCA  >  NC_002947/584971‑585263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑