Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 596,124 G→C 8.5% S10R (AGC→AGG)  PP_0506 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947596,1240GC 8.5% 182.9 / 6.7 59S10R (AGC→AGG) PP_0506ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (31/23);  new base C (2/3);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

GTGCACCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGGCGGCGCGG  >  NC_002947/595976‑596270
                                                                                                                                                    |                                                                                                                                                   
gTGCACCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAgg                                                                                                                                                     <  7:338052/149‑1 (MQ=255)
  gCACCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGTGGTCAGAAACGCAGTGAATCGGCGGTTGGCACGGCt                                                                                                                                                   >  6:269879/1‑149 (MQ=255)
    aCCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTg                                                                                                                                                  <  8:230399/148‑1 (MQ=255)
    aCCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTg                                                                                                                                                  >  7:230399/1‑148 (MQ=255)
      cGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGc                                                                                                                                                >  6:147421/1‑148 (MQ=255)
      cGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGcc                                                                                                                                               <  5:294774/149‑1 (MQ=255)
      cGACTATCAGGTCGGTGCAGCTGATGGTGGTGGCGAAGCTGGCACGGTCTTCGGTACGGAGCAGCTCTACAGCGAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGcc                                                                                                                                               <  4:188819/149‑1 (MQ=255)
       gACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGcc                                                                                                                                               >  2:209576/1‑148 (MQ=255)
        aCTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGcc                                                                                                                                               >  4:51753/1‑147 (MQ=255)
        aCTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCcaa                                                                                                                                             <  1:197533/149‑1 (MQ=255)
            tCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCATTGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCaaaggca                                                                                                                                         >  4:71777/1‑149 (MQ=255)
            tCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCcaaggc                                                                                                                                          >  7:224728/1‑148 (MQ=255)
            tCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaaccgc                                                                                                                                         <  5:266991/149‑5 (MQ=255)
            tCAGGTCCGTGCCGCTGATGGTCCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCTGTCAGAAACGCAGTGAATCGGCGGTTGCCCAGGCTGGCcaaggc                                                                                                                                          >  8:100425/1‑148 (MQ=255)
                gTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCaaggcaaggc                                                                                                                                     >  7:45887/1‑149 (MQ=255)
                      cacGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCa                                                                                                                               <  1:162418/147‑1 (MQ=255)
                       ccGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCa                                                                                                                               <  1:215552/148‑1 (MQ=255)
                       ccGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAg                                                                                                                              <  3:232382/149‑1 (MQ=255)
                       ccGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAg                                                                                                                              <  1:176891/149‑1 (MQ=255)
                          cTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGCCAGAAACGCAGTGAATCGGCGGTTGGCCAGCCTGGCcaaccg                                                                                                                                          <  5:261331/134‑4 (MQ=255)
                                    tGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCt                                                                                                                 >  4:55502/1‑149 (MQ=255)
                                      gCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTcc                                                                                                               >  2:345529/1‑149 (MQ=255)
                                        gAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACTCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCg                                                                                                             <  8:45887/149‑1 (MQ=255)
                                               gCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTg                                                                                                      >  3:244550/1‑149 (MQ=255)
                                               gCACGGGCTTCGGTACGGACCCGCTCAACACCCAGCAGCAGGCACACCGACAGGGCTATGGCAAAGGCGGTCAGAAACGCAGTGATTCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTg                                                                                                      <  7:100425/149‑1 (MQ=255)
                                                    ggCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTggcggc                                                                                                 <  3:51753/149‑1 (MQ=255)
                                                       ttCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGcgcg                                                                                              >  1:2278/1‑149 (MQ=255)
                                                                gACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTc                                                                                     <  3:55502/149‑1 (MQ=255)
                                                                 aCCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAgg                                                                                                                                                     <  7:364515/84‑1 (MQ=255)
                                                                 aCCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGc                                                                                                                   <  8:14775/118‑1 (MQ=255)
                                                                 aCCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGc                                                                                                                   >  7:14775/1‑118 (MQ=255)
                                                                 aCCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCg                                                                                    <  2:374087/149‑1 (MQ=255)
                                                                  cccGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGTGGTCAGAAACGCAGTGAATCGGCGGTTGGCAAGGCTGGCCAAGGCAAGTCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCgg                                                                                   <  5:269879/149‑1 (MQ=255)
                                                                   ccGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCgg                                                                                   >  4:17007/1‑148 (MQ=255)
                                                                   ccGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCgg                                                                                   >  3:326809/1‑148 (MQ=255)
                                                                           cagccagcAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCAAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑a                                                                           >  6:275667/1‑147 (MQ=255)
                                                                             gccagcAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAgg                                                                       <  5:147421/149‑1 (MQ=255)
                                                                              ccagcAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGt                                                                      >  4:257524/1‑149 (MQ=255)
                                                                              ccagcAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGAGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGt                                                                      >  1:306733/1‑149 (MQ=255)
                                                                                 gcagcagGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGc                                                                   <  3:257524/149‑1 (MQ=255)
                                                                                               gACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAgcggc                                                     >  3:223002/1‑149 (MQ=255)
                                                                                                aCAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAgcggcg                                                    >  5:74151/1‑149 (MQ=255)
                                                                                                  aGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGcc                                                  <  4:244550/149‑1 (MQ=255)
                                                                                                    ggCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCGCACAGGTGGCGGTCGAACAGCGGCGCCa                                                 <  1:209576/149‑1 (MQ=255)
                                                                                                                   ggTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGt                                     >  1:73220/1‑145 (MQ=255)
                                                                                                                   ggTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGt                                     <  2:73220/145‑1 (MQ=255)
                                                                                                                    gTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTgg                                         >  6:368889/1‑140 (MQ=255)
                                                                                                                    gTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTgg                                         <  5:368889/140‑1 (MQ=255)
                                                                                                                     tCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGa                               >  3:6479/1‑149 (MQ=255)
                                                                                                                        gAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGGAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCTAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGGTCTGGTCGTGGCTGACGa                            >  8:185737/1‑149 (MQ=255)
                                                                                                                         aaaCGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGaa                           >  5:348503/1‑149 (MQ=255)
                                                                                                                           aCGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGaa                           >  2:171129/1‑147 (MQ=255)
                                                                                                                                  gAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCt                  >  3:168489/1‑149 (MQ=255)
                                                                                                                                   aaTCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGCACAGCAGGCTg                 <  1:345529/149‑1 (MQ=255)
                                                                                                                                   aaTCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTg                 <  8:224728/149‑1 (MQ=255)
                                                                                                                                    aTCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTgg                >  7:94709/1‑149 (MQ=255)
                                                                                                                                       ttgccttGGCCAGCCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCAc             >  5:211552/6‑149 (MQ=255)
                                                                                                                                        gcggTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGTGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCAcc            >  6:163623/1‑149 (MQ=255)
                                                                                                                                            ttGGCCAGCCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACcggcg        >  5:372003/1‑149 (MQ=255)
                                                                                                                                               gCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGgcggcg     >  4:107534/1‑149 (MQ=255)
                                                                                                                                                  aGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGgcggcgcgg  <  3:17007/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGCACCGACTATCAGGTCGGTGCCGCTGATGGTGCTGGCGAAGCTGGCACGGGCTTCGGTACGGACCCGCTCAACAGCCAGCAGCAGGCACACCGACAGGGCAATGGCAAAGGCGGTCAGAAACGCAGTGAATCGGCGGTTGGCCAGGCTGGCCAAGGCAAGGCGGAGCAGGTACATCAGGCCTCCCGGGGCTTGGCGGCGCGGTTCAGTTCGGCAAGCG‑ACAGGTGGCGGTCGAACAGCGGCGCCAGGCTCTGGTCGTGGCTGACGAACAGCAGGCTGGCACCGGCGGCGCGG  >  NC_002947/595976‑596270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑