BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	607,383	T→A	7.8%	I96N (ATC→AAC) ‡	ribA →	GTP cyclohydrolase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	607,383	0	T	A	7.8%	287.6 / 10.5	102	I96N (ATC→AAC) ‡	ribA	GTP cyclohydrolase II
Reads supporting (aligned to +/- strand): ref base T (47/45); new base A (2/6); total (50/52)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 102 total aligned reads displayed.

GGGCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAGTCGC  >  NC_002947/607238‑607529

gggCGCCTGTATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCgg                                                                                                                                                 >  4:209689/1‑149 (MQ=255)

    gcCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGATGCCACGGCctt                                                                                                                                             >  1:377327/1‑148 (MQ=255)

     ccTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCctgc                                                                                                                                            >  3:166617/1‑149 (MQ=255)

     ccTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTCCGTCAG‑‑GAAGGCCGATGCCACGGCcttc                                                                                                                                            <  7:44893/149‑3 (MQ=255)

      cTGCATTCCGAGTTCCTGACCGGCGATGCAATGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGATGCCACGGCcttcc                                                                                                                                           <  5:75074/149‑4 (MQ=255)

      cTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTGGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGAGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGATGCCACGGCcttcc                                                                                                                                           <  4:57518/149‑4 (MQ=255)

      cTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGATGCCACGGCcttcc                                                                                                                                           <  2:377327/149‑4 (MQ=255)

      cTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGACTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGATGCCACGGCcttcc                                                                                                                                           <  7:201003/149‑4 (MQ=255)

       tGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCctgct                                                                                                                                           >  6:254044/1‑148 (MQ=255)

       tGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCACCGGCctgct                                                                                                                                           >  6:360799/1‑148 (MQ=255)

         cATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGaa                                                                                                                                        <  2:362981/149‑1 (MQ=255)

          aTTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAAc                                                                                                                                       <  8:274279/149‑1 (MQ=255)

           ttCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAAc                                                                                                                                       >  5:188227/1‑148 (MQ=255)

           ttCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACa                                                                                                                                      <  6:188227/149‑1 (MQ=255)

               gAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAAc                                                                                                                                       >  3:365414/1‑144 (MQ=255)

                 gTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCACCGGCCTGCTGAACAAGATcc                                                                                                                                <  5:360799/149‑1 (MQ=255)

                     cTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCgcgc                                                                                                                            >  1:12455/1‑149 (MQ=255)

                       gACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGcc                                                                                                                           >  1:203975/1‑148 (MQ=255)

                        aCCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTa                                                                                                                         >  2:7875/1‑149 (MQ=255)

                          cGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAATGCCGATGCCACGGCcttcct                                                                                                                                          >  6:5853/1‑126 (MQ=255)

                          cGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTAc                                                                                                                        >  3:309769/1‑148 (MQ=255)

                          cGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACg                                                                                                                       <  3:209689/149‑1 (MQ=255)

                          cGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGATGCCACGGCcttcct                                                                                                                                          <  5:5853/130‑5 (MQ=255)

                           ggCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTAc                                                                                                                        >  5:73842/1‑147 (MQ=255)

                           ggCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGa                                                                                                                      <  3:218787/149‑1 (MQ=255)

                            gCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAAATCCGCGCCTACGAg                                                                                                                     >  1:327702/1‑149 (MQ=255)

                                  cACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCa                                                                                                                <  8:41869/148‑1 (MQ=255)

                                  cACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAg                                                                                                               >  8:234668/1‑149 (MQ=255)

                                   aCTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAg                                                                                                               >  8:348069/1‑148 (MQ=255)

                                    cTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATcc                                                                                                                                <  3:134226/130‑1 (MQ=255)

                                    cTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATcc                                                                                                                                >  4:134226/1‑130 (MQ=255)

                                       ttCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATgg                                                                                                          <  2:327702/149‑1 (MQ=255)

                                         cagccagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATggc                                                                                                         <  8:190717/148‑1 (MQ=255)

                                          agccagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGc                                                                                                                 >  7:164127/1‑139 (MQ=255)

                                          agccagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGc                                                                                                                 <  8:164127/139‑1 (MQ=255)

                                          agccagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATggcgg                                                                                                       >  3:232317/1‑149 (MQ=255)

                                           gccagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATggcggc                                                                                                      >  3:194543/1‑149 (MQ=255)

                                             cagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGgcgc                                                                                                    <  8:4377/149‑1 (MQ=255)

                                              agcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGc                                                                                                                 >  2:272109/1‑135 (MQ=255)

                                              agcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGcc                                                                                                   >  5:68779/1‑149 (MQ=255)

                                              agcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAACGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGc                                                                                                                 <  1:272109/135‑1 (MQ=255)

                                               gcgcTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGcc                                                                                                   >  2:102688/1‑148 (MQ=255)

                                                       aCTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTc                                                                                          <  4:365414/149‑1 (MQ=255)

                                                       aCTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTc                                                                                          <  7:348069/149‑1 (MQ=255)

                                                        cTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTc                                                                                          >  1:347373/1‑148 (MQ=255)

                                                        cTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCg                                                                                         <  7:160965/149‑1 (MQ=255)

                                                         tGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGTCGAGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCg                                                                                         >  1:324399/1‑148 (MQ=255)

                                                                 gCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGc                                                                                     >  8:295936/1‑144 (MQ=255)

                                                                 gCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGc                                                                                     <  7:295936/144‑1 (MQ=255)

                                                                 gCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAAc                                                                                 >  7:342063/1‑148 (MQ=255)

                                                                  cAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAAc                                                                                 >  8:40006/1‑147 (MQ=255)

                                                                  cAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGa                                                                               <  7:272867/149‑1 (MQ=255)

                                                                     ttGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAgcg                                                                            >  3:50360/1‑149 (MQ=255)

                                                                       ggAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGATGAAGGCCGTGCCATCGCCCTGAGGAACAAGAACCCCCCCTACGCGCTGAAGGATGGCGGCGCCCACACCGAccaacccaaccaccc                                                                            >  1:112166/1‑144 (MQ=255)

                                                                       ggAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGCAGCCAACGAGCGcc                                                                          >  6:124791/1‑149 (MQ=255)

                                                                         aaGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTg                                                                        <  4:50360/149‑1 (MQ=255)

                                                                         aaGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGAGCCGACACCGTCGAAGCCAACGAGCGCCTg                                                                        >  7:23019/1‑149 (MQ=255)

                                                                            ccGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCGGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGc                                                                     <  2:58574/149‑1 (MQ=255)

                                                                             cgcgCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCt                                                                    >  7:284082/1‑149 (MQ=255)

                                                                                    cAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTg                                                              <  2:347373/148‑1 (MQ=255)

                                                                                    cAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGc                                                             >  1:108389/1‑149 (MQ=255)

                                                                                      ggCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCg                                                           <  2:203975/149‑1 (MQ=255)

                                                                                      ggCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCg                                                           <  6:73842/149‑1 (MQ=255)

                                                                                         cATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCg                                                                 <  4:354857/140‑1 (MQ=255)

                                                                                         cATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCg                                                                 >  3:354857/1‑140 (MQ=255)

                                                                                           tCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAg                                                      >  7:293503/1‑149 (MQ=255)

                                                                                             gCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCCTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGCGCTTCGCTGCCGACCAGCg                                                    >  4:252073/1‑149 (MQ=255)

                                                                                               ccGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGt                                                   >  1:247108/1‑148 (MQ=255)

                                                                                                cgcgAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATggc                                                                                                         >  5:107954/1‑93 (MQ=255)

                                                                                                cgcgAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATggc                                                                                                         <  6:107954/93‑1 (MQ=255)

                                                                                                cgcgAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGa                                                 <  1:7875/149‑1 (MQ=255)

                                                                                                         cGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTgg                                                                       >  8:189252/1‑118 (MQ=255)

                                                                                                         cGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTgg                                                                       <  7:189252/118‑1 (MQ=255)

                                                                                                          gTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCata                                       <  4:194543/149‑1 (MQ=255)

                                                                                                           tGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCatat                                      >  8:138020/1‑149 (MQ=255)

                                                                                                             gggTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCt                                                               <  6:10704/122‑1 (MQ=255)

                                                                                                               gtgtTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATAtgcct                                  <  3:266961/149‑1 (MQ=255)

                                                                                                                  ttgttgtACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATAtgcctgc                                <  4:166617/148‑1 (MQ=255)

                                                                                                                     ttgtACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCTTCGAAGGCAACGAGCGCCTGGTCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGAt                            <  3:252073/149‑1 (MQ=255)

                                                                                                                     ttgtACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGa                             <  7:338696/148‑1 (MQ=255)

                                                                                                                     ttgtACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGAt                            >  7:61014/1‑149 (MQ=255)

                                                                                                                          ccTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTc                        <  1:102688/148‑1 (MQ=255)

                                                                                                                          ccTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTc                        <  5:254044/148‑1 (MQ=255)

                                                                                                                          ccTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCg                       <  2:324399/149‑1 (MQ=255)

                                                                                                                           cTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCTGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTc                        >  2:41310/1‑147 (MQ=255)

                                                                                                                             gCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAg                     >  2:94884/1‑148 (MQ=255)

                                                                                                                              cGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCg                                                                 <  6:235829/103‑1 (MQ=255)

                                                                                                                              cGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCg                                                                 >  5:235829/1‑103 (MQ=255)

                                                                                                                              cGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAg                     <  8:293503/147‑1 (MQ=255)

                                                                                                                               gTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCCGCCGATGCTCGAGCAc                  <  4:232317/149‑1 (MQ=255)

                                                                                                                                tCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCAcc                 >  7:8783/1‑149 (MQ=255)

                                                                                                                                 cAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCt                >  6:360651/1‑149 (MQ=255)

                                                                                                                                   g‑‑gAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTgg              >  8:349189/1‑149 (MQ=255)

                                                                                                                                       aaGGCGGTGGCATCGGCGTGCTGAACAAGATGCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGc            <  2:124662/149‑1 (MQ=255)

                                                                                                                                       aaGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGc            <  2:108389/149‑1 (MQ=255)

                                                                                                                                          gCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTc         <  6:68779/149‑1 (MQ=255)

                                                                                                                                          gCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTc         <  7:234668/149‑1 (MQ=255)

                                                                                                                                            cGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCaa       <  8:342063/149‑1 (MQ=255)

                                                                                                                                              tGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAGt     >  2:59639/1‑149 (MQ=255)

                                                                                                                                                 cATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAGTCGc  <  2:247108/149‑1 (MQ=255)

GGGCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAG‑‑GAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAGTCGC  >  NC_002947/607238‑607529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`