Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 616,450 A→C 7.1% noncoding (72/142 nt) PP_mr11 ← FMN regulatory region

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947616,4500AC 7.1% 175.4 / 3.4 56noncoding (72/142 nt)PP_mr11FMN regulatory region
Reads supporting (aligned to +/- strand):  ref base A (29/23);  new base C (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GAAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAGT  >  NC_002947/616302‑616594
                                                                                                                                                    |                                                                                                                                                
gAAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACa                                                                                                                                                  >  1:371380/1‑149 (MQ=255)
     gCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTc                                                                                                                                             <  3:312870/149‑1 (MQ=255)
         ctcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggg                                                                                                                                         <  3:258017/149‑1 (MQ=255)
         ctcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCgg                                                                                                                                           <  1:10230/147‑1 (MQ=255)
          tcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGgc                                                                                                                                        >  2:58706/1‑149 (MQ=255)
                 tGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTcgcg                                                                                                                                 >  1:225872/1‑149 (MQ=255)
                   aTCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCgg                                                                                                                                <  7:282251/148‑1 (MQ=255)
                          aaCGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggggtcag                                                                                                                                    >  6:315511/1‑132 (MQ=255)
                          aaCGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggggtcag                                                                                                                                    <  5:315511/137‑6 (MQ=255)
                                   gggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGgcgc                                                                                                                                      <  8:64879/126‑1 (MQ=255)
                                       gAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGCCCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTAccgc                                                                                                           >  3:169650/1‑149 (MQ=255)
                                         aCAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTAccgccg                                                                                                         <  6:25153/149‑1 (MQ=255)
                                                 cgcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGa                                                                                                  <  7:243287/148‑1 (MQ=255)
                                                    gcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGCGCTCATCTATCGATTTACCGCCGGTGGGGACTTa                                                                                              >  8:247032/1‑149 (MQ=255)
                                                        aaGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACAcc                                                                                          >  7:12143/1‑149 (MQ=255)
                                                          gggCGCCCGGATGGCCCCGGCATGACGCCTTCTTTCATCCGGACTATGCCCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACAcccc                                                                                        <  2:322710/149‑1 (MQ=255)
                                                           ggCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCg                                                                                       >  6:230553/1‑149 (MQ=255)
                                                             cgcCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGcc                                                                                     <  2:371380/149‑1 (MQ=255)
                                                                  ggATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                                <  2:225872/149‑1 (MQ=255)
                                                                  ggATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                                <  1:58706/149‑1 (MQ=255)
                                                                       gCCCCTTCATGTCGCCTTATTTCATCCGGACTATTAGCGTCGGCTCTGGAGTTTCACCAGATCTTCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgacc                                                                            <  2:369484/148‑1 (MQ=255)
                                                                         cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgaccgac                                                                         >  4:374782/1‑149 (MQ=255)
                                                                         cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgac                                                                             <  2:351469/145‑1 (MQ=255)
                                                                         cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgac                                                                             >  1:351469/1‑145 (MQ=255)
                                                                          ccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgaccgacc                                                                        >  8:236105/1‑149 (MQ=255)
                                                                               aTGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACAGACCCAGc                                                                    >  7:360024/1‑148 (MQ=255)
                                                                                    gCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAg                                                              >  4:294722/1‑149 (MQ=255)
                                                                                     ccTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAg                                                              >  3:15754/1‑148 (MQ=255)
                                                                                         cttTCATCCGGACTATGACCGTCGTCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGcaca                                                          <  1:66365/148‑1 (MQ=255)
                                                                                          tttCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGt                                                        >  4:378856/1‑149 (MQ=255)
                                                                                               tCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAc                                                   >  7:193765/1‑149 (MQ=255)
                                                                                                      tATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTg                                            >  8:350047/1‑149 (MQ=255)
                                                                                                         gACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTAACGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCAAGCATACAGCACAGTGCCACCCCAGTGGc                                          >  6:217301/1‑148 (MQ=255)
                                                                                                           ccGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTC‑GGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAAcc                                      >  4:29861/1‑149 (MQ=255)
                                                                                                                  ctctGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGt                                <  7:236105/149‑1 (MQ=255)
                                                                                                                       gagaTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTctac                           <  3:50567/149‑1 (MQ=255)
                                                                                                                       gAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCTTTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCA‑CCCAGTGGCAACCCGCGGTTctac                           <  4:169650/148‑1 (MQ=255)
                                                                                                                        aGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTctact                          <  3:378856/149‑1 (MQ=255)
                                                                                                                             cACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCtt                     >  2:196824/1‑149 (MQ=255)
                                                                                                                               ccAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCg                   >  6:264771/1‑149 (MQ=255)
                                                                                                                               ccAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCg                   >  1:67584/1‑149 (MQ=255)
                                                                                                                                cAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCgg                  >  1:384006/1‑149 (MQ=255)
                                                                                                                                 aGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGc                 <  8:360024/149‑1 (MQ=255)
                                                                                                                                 aGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGc                 <  3:294722/149‑1 (MQ=255)
                                                                                                                                       gctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACt           >  4:133324/1‑149 (MQ=255)
                                                                                                                                        ctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                                >  4:245500/1‑79 (MQ=255)
                                                                                                                                        ctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                                <  3:245500/79‑1 (MQ=255)
                                                                                                                                        ctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCgaga             <  7:247032/146‑1 (MQ=255)
                                                                                                                                           aCCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgca       <  5:230553/149‑1 (MQ=255)
                                                                                                                                            gccccGACCATGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag      >  1:2387/2‑149 (MQ=255)
                                                                                                                                             ccccGACATGGTCGGGGCGCTCGCGGGCTCATGTTTCGATTTACCGCCGGTGGGGACGTACACCCCGCCCTGCAGACCGACCCAGCATACAGCACAGTGCCACCGCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag      >  3:295999/1‑148 (MQ=255)
                                                                                                                                             ccccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag      >  8:15004/1‑148 (MQ=255)
                                                                                                                                             ccccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag      >  3:366892/1‑148 (MQ=255)
                                                                                                                                             ccccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag      >  2:19905/1‑148 (MQ=255)
                                                                                                                                               ccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGcagcag   >  8:147217/1‑149 (MQ=255)
                                                                                                                                               ccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGcagcag   >  1:260750/1‑149 (MQ=255)
                                                                                                                                                cGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAGt  <  4:15754/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GAAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAGT  >  NC_002947/616302‑616594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑