Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 685,574 G→T 6.0% L225M (CTG→ATG)  cadA‑II ← cadmium translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947685,5740GT 6.0% 260.1 / 3.2 80L225M (CTG→ATG) cadA‑IIcadmium translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (42/33);  new base T (3/2);  total (45/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGT  >  NC_002947/685426‑685722
                                                                                                                                                    |                                                                                                                                                    
tGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACAAGCGCTGGTGCCCAg                                                                                                                                                      >  4:235922/1‑149 (MQ=255)
  gCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAggg                                                                                                                                                    >  4:58751/1‑149 (MQ=255)
  gCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAgg                                                                                                                                                     <  5:387414/148‑1 (MQ=255)
  gCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAg                                                                                                                                                      <  8:43214/147‑1 (MQ=255)
  gCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCACAGGCCATAACCAGCGCTGGTGCCCAgg                                                                                                                                                     <  1:220860/148‑1 (MQ=255)
  gCGGTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAg                                                                                                                                                      <  4:240321/147‑1 (MQ=255)
           gcgGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACcag                                                                                                                                             >  5:28823/1‑147 (MQ=255)
            cgGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGGCCCTGGGCACcagcgc                                                                                                                                          <  4:24198/149‑3 (MQ=255)
            cgGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACcagcag                                                                                                                                          <  3:58751/149‑1 (MQ=255)
            cgGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACcagcag                                                                                                                                          <  7:57552/149‑1 (MQ=255)
                tttCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTc                                                                                                                                       >  6:82413/1‑148 (MQ=255)
                tttCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTc                                                                                                                                       >  8:325960/1‑148 (MQ=255)
                 ttCCAGGTACTTGCCCAGCAATACCAGGGCAATGACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCa                                                                                                                                     >  4:59629/1‑149 (MQ=255)
                   ccAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCAt                                                                                                                                    <  5:82413/148‑1 (MQ=255)
                    cAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGt                                                                                                                                  >  2:38360/1‑149 (MQ=255)
                     aGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAgg                                                                                                                                                     <  7:263952/129‑1 (MQ=255)
                     aGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAgg                                                                                                                                                     >  8:263952/1‑129 (MQ=255)
                     aGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGtt                                                                                                                                 >  2:9781/1‑149 (MQ=255)
                            tGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccggcg                                                                                                                          >  7:128054/1‑149 (MQ=255)
                             gCCCAGCAATACCAGGGCAATTACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccggc                                                                                                                           <  3:59629/147‑1 (MQ=255)
                             gCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACAAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGtt                                                                                                                                 <  8:322705/141‑1 (MQ=255)
                             gCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACAAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGtt                                                                                                                                 >  7:322705/1‑141 (MQ=255)
                               ccAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccggcgccg                                                                                                                       >  3:78172/1‑149 (MQ=255)
                               ccAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccggcgccg                                                                                                                       <  8:268618/149‑1 (MQ=255)
                               ccAGCAATACCAGGGCAATCACCACGGCTGACGCGTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGAGCTGCTGCCCAGGGCCACCAGCAGGACCATGTTgccggcgccg                                                                                                                       >  7:171148/1‑149 (MQ=255)
                                  gCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGccggcgccggcg                                                                                                                    >  3:351093/1‑149 (MQ=255)
                                   cAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCcggcgccggcgc                                                                                                                   >  2:166848/1‑149 (MQ=255)
                                       aCCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCAc                                                                                                               >  2:9755/1‑149 (MQ=255)
                                            ggCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCt                                                                                                          >  4:163909/1‑149 (MQ=255)
                                               aaTCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTcc                                                                                                       <  3:163909/149‑1 (MQ=255)
                                               aaTCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTc                                                                                                        >  6:107887/1‑148 (MQ=255)
                                                aTCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCa                                                                                                      <  8:163535/149‑1 (MQ=255)
                                                    ccacGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccg                                                                                                                             <  6:261038/122‑1 (MQ=255)
                                                    ccacGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTgccg                                                                                                                             >  5:261038/1‑122 (MQ=255)
                                                    ccacGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCAGGCGGGAGCCTGTGACCAGTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCATGGCCACCAGCATGTACATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCt                                                                                                  >  5:88380/1‑149 (MQ=255)
                                                    ccacGGCTGAGGCTTCGAAGTAAATGTGCGGTGCCATGCCGTCGGGAGCCTCTTCCCATTGGTACAGGCTCAGCACATAACCAGCGCTGGTGACCAGGGCCACCAGCAGGACCATGTATACGGCGCCCGCGCGCACGGCCTACCAGGcg                                                                                                  >  8:344542/1‑148 (MQ=255)
                                                             aGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATg                                                                                            >  7:291273/1‑146 (MQ=255)
                                                             aGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTa                                                                                          <  1:166848/148‑1 (MQ=255)
                                                                   cGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGc                                                                                     <  2:45990/147‑1 (MQ=255)
                                                                   cGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGc                                                                                     <  4:78172/147‑1 (MQ=255)
                                                                   cGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCgg                                                                                   >  8:43064/1‑149 (MQ=255)
                                                                    gAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCggg                                                                                  >  8:130103/1‑149 (MQ=255)
                                                                        tACAGGTGCGGGGCAATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCGCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCAcc                                                                              >  6:86410/1‑149 (MQ=255)
                                                                          cAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCga                                                                            <  3:324262/149‑1 (MQ=255)
                                                                             gTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGaa                                                                         >  1:245958/1‑149 (MQ=255)
                                                                              tGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAAt                                                                        <  7:325960/149‑1 (MQ=255)
                                                                               gCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATg                                                                       <  3:235922/149‑1 (MQ=255)
                                                                                 ggggCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGaa                                                                     <  6:28823/149‑1 (MQ=255)
                                                                                  gggCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAAc                                                                    >  5:64168/1‑149 (MQ=255)
                                                                                        tGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCCCGGCCTTCCAGGATGCAATGTAGAAGCGGGCACCGAGCATGAACTGCACg                                                              >  8:280159/1‑149 (MQ=255)
                                                                                          ccGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACgg                                                             <  1:9755/148‑1 (MQ=255)
                                                                                           cggcggGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGgt                                                           >  4:113940/1‑149 (MQ=255)
                                                                                              cggGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGtgtg                                                        <  1:38360/149‑1 (MQ=255)
                                                                                               gggAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTgg                                                       >  2:207396/1‑149 (MQ=255)
                                                                                                   gCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTACAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcag                                                   <  7:92304/149‑1 (MQ=255)
                                                                                                          cccATTGGTACAGGCTGAGGCCATAACAAGCGCTGGTGCCCAGGGCCTCCAGCAGGTCGATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCGGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGaa                                             <  6:88380/148‑1 (MQ=255)
                                                                                                             aTTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACCgc                                         <  3:371348/149‑1 (MQ=255)
                                                                                                                 gTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAAGTGCACGGGTGTGGCCAGCAGGAACTGCGcc                                      <  1:32733/148‑1 (MQ=255)
                                                                                                                 gTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagca                                                 >  4:252561/1‑137 (MQ=255)
                                                                                                                 gTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagca                                                 <  3:252561/137‑1 (MQ=255)
                                                                                                                 gTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagca                                                 >  4:157875/1‑137 (MQ=255)
                                                                                                                 gTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCcagca                                                 <  3:157875/137‑1 (MQ=255)
                                                                                                                   aCAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAg                                   >  3:344375/1‑149 (MQ=255)
                                                                                                                    cAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAgg                                  <  7:130103/149‑1 (MQ=255)
                                                                                                                     aGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGc                                 <  5:107887/149‑1 (MQ=255)
                                                                                                                       gCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAAc                                                                    <  5:212329/112‑1 (MQ=255)
                                                                                                                       gCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAAc                                                                    >  6:212329/1‑112 (MQ=255)
                                                                                                                       gCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCgg                               >  8:97772/1‑149 (MQ=255)
                                                                                                                       gCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCgg                               >  3:120661/1‑149 (MQ=255)
                                                                                                                       gCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCgg                               >  6:138847/1‑149 (MQ=255)
                                                                                                                             gCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAg                           >  6:356878/1‑147 (MQ=255)
                                                                                                                              ccATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCAt                        >  8:69597/1‑149 (MQ=255)
                                                                                                                               cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc                       <  4:351093/149‑1 (MQ=255)
                                                                                                                               cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc                       <  7:149784/149‑1 (MQ=255)
                                                                                                                               cATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc                       <  7:43064/149‑1 (MQ=255)
                                                                                                                                aTAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATc                       >  3:358500/1‑148 (MQ=255)
                                                                                                                                 tAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGGCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCa                     <  6:121323/149‑1 (MQ=255)
                                                                                                                                    ccAGTGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTg                  >  7:56105/1‑149 (MQ=255)
                                                                                                                                    ccAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                    >  2:97913/1‑67 (MQ=255)
                                                                                                                                    ccAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAgg                                                                                                    <  1:97913/67‑1 (MQ=255)
                                                                                                                                     cAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTg                  >  3:321895/1‑148 (MQ=255)
                                                                                                                                     cAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCAGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTgc                                         >  7:6679/1‑125 (MQ=255)
                                                                                                                                     cAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCAGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTgc                                         <  8:6679/125‑1 (MQ=255)
                                                                                                                                       ctgcTGGTGGCCCTGGGCACCAGCGGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTa                            <  2:230512/134‑1 (MQ=255)
                                                                                                                                       ctgcTGGTGGCCCTGGGCACCAGCGGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTa                            >  1:230512/3‑136 (MQ=255)
                                                                                                                                          cTGGTGGCCCTGGGCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTgc                                         >  2:278363/1‑120 (MQ=255)
                                                                                                                                               gCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACg       >  6:227610/1‑149 (MQ=255)
                                                                                                                                                  cAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGtt    >  7:275396/1‑149 (MQ=255)
                                                                                                                                                    gggCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGt  <  4:358500/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCGTTTGGCGCGGCTTTCCAGGTACTTGCCCAGCAATACCAGGGCAATCACCACGGCTGAGGCTTCGAAGTACAGGTGCGGGGCCATGCCGGCGGGAGCCTGTGCCCATTGGTACAGGCTCAGGCCATAACCAGCGCTGGTGCCCAGGGCCACCAGCAGGTCCATGTTGCCGGCGCCGGCGCGCACGGCCTTCCAGGCTGCAATGTAGAAGCGGGCACCGAGAATGAACTGCACGGGTGTGGCCAGCAGGAACTGCGCCCAGGCGGGTAGCATCCAGTGCAAGCCGAACGGTTGT  >  NC_002947/685426‑685722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑