Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	711,370	G→C	6.8%	A390P (GCG→CCG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	711,370	0	G	C	6.8%	189.8 / 4.1	59	A390P (GCG→CCG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base C (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ACCTGCAAGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGCGTG  >  NC_002947/711223‑711511                                                                                                                                                     |                                                                                                                                              aCCTGCAAGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTgc                                                                                                                                              >  2:251845/1‑149 (MQ=255)     gCAAGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGAATGTCTACTCGCTGCCATTCTTCCGCGGCCAGTTCAACTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGACGGTGCGCt                                                                                                                                           >  7:296212/1‑148 (MQ=255)      cAAGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGa                                                                                                                                         >  2:320752/1‑149 (MQ=255)       aaGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGTGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCTCTGAt                                                                                                                                        <  5:267198/149‑1 (MQ=255)       aaGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGAt                                                                                                                                        <  8:86949/149‑1 (MQ=255)         gcgcTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGc                                                                                                                                      <  6:345524/149‑1 (MQ=255)              aCGGCATGGTCCACGACGACATCCTTACCCCGCTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACAcc                                                                                                                                 <  4:285536/149‑1 (MQ=255)               cGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCacac                                                                                                                                  <  1:320752/147‑1 (MQ=255)                 gCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCTATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGa                                                                                                                               >  8:256657/1‑148 (MQ=255)                 gCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGaa                                                                                                                              <  6:167750/149‑1 (MQ=255)                 gCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGa                                                                                                                               >  4:348221/1‑148 (MQ=255)                      gTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGG‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTg                                                                                                                                               <  4:160690/127‑1 (MQ=255)                      gTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTg                                                                                                                                               >  3:160690/1‑127 (MQ=255)                       tCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATc                                                                                                                        <  8:53339/149‑1 (MQ=255)                        cAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCAt                                                                                                                         >  3:256172/1‑147 (MQ=255)                           cgacgaCATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGcc                                                                                                                    <  4:256172/149‑1 (MQ=255)                              cgacATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCca                                                                                                                   >  1:21190/1‑147 (MQ=255)                                  aTCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGCTTAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCt                                                                                                              <  1:314686/149‑1 (MQ=255)                                   tCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTAc                                                                                                            <  4:192778/149‑1 (MQ=255)                                    ccTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CCACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGCTGCGCTGATGCACCCCGAAACCATCAGCCACAGCTTCa                                                                                                           >  4:101048/1‑149 (MQ=255)                                        aCCCCGGTGCAGAGCCACGGCGTGTATGTCGACTCGCTGCCAGTCTTCGGCGGCCAGTTCATCTGGACGGC‑GCACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGGACAGGGCAGCCATCAGCGAGAGCTCCAAGCa                                                                                                       >  6:248018/1‑149 (MQ=255)                                         ccccGGTGCAGAGTAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCAc                                                                                                      <  1:92190/149‑1 (MQ=255)                                                   gagCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGgcgc                                                                                             >  3:134547/1‑148 (MQ=255)                                                   gagCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGcc                                                                                            <  4:327527/149‑1 (MQ=255)                                                    agCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCca                                                                                           >  6:76288/1‑149 (MQ=255)                                                     gCAACGGCGTGTATGTCGACTCGCTTCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCTGCCTTCGTCGAGAAGCTGAGCGAAGTCGGTGCTCTGATGCACACCGAAACCATCTGCCACAGCTACATGCACTGCTGGCGCcac                                                                                          <  8:296212/149‑1 (MQ=255)                                                             gtgtATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGAcccc                                                                                  >  4:331354/1‑149 (MQ=255)                                                                tATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTCCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCt                                                                               >  6:30373/1‑149 (MQ=255)                                                                tATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTCCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCt                                                                               >  6:24946/1‑149 (MQ=255)                                                                    tCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATc                                                                           <  1:251845/149‑1 (MQ=255)                                                                           gCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACcgcg                                                                    >  1:183749/1‑149 (MQ=255)                                                                           gCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACcgcg                                                                    >  7:64071/1‑149 (MQ=255)                                                                                       cggcggCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCgcgc                                                           <  3:331354/146‑1 (MQ=255)                                                                                        ggcggcCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTg                                                       <  6:312260/149‑1 (MQ=255)                                                                                        ggcggcCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTg                                                       <  5:299672/149‑1 (MQ=255)                                                                                         gcggcCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACaa                                                                                        <  7:354793/115‑1 (MQ=255)                                                                                         gcggcCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACaa                                                                                        >  8:354793/1‑115 (MQ=255)                                                                                            gcCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTc                                                   >  1:235741/1‑149 (MQ=255)                                                                                               aGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTg                                                >  2:247409/1‑149 (MQ=255)                                                                                                    aTCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCa                                            <  2:235741/148‑1 (MQ=255)                                                                                                    aTCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCAt                                           <  3:348221/149‑1 (MQ=255)                                                                                                     tCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATg                                          >  4:288277/1‑149 (MQ=255)                                                                                                      cTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATg                                          >  8:212280/1‑148 (MQ=255)                                                                                                           aGGC‑AAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAag                                    <  6:165329/149‑1 (MQ=255)                                                                                                            ggC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGCCCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGCCAag                                    >  2:324270/1‑148 (MQ=255)                                                                                                                   cccGGCCAGCGTCGAGAAGCTGAGCGAAGGCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGa                             <  4:134547/149‑1 (MQ=255)                                                                                                                   cccGCCCATCGGCGAGACGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTTCTGGCGCCCCAATCCCCAGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTTGGCATTGACAAGCAGCCGa                             <  8:171643/149‑1 (MQ=255)                                                                                                                     cGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGc                           <  4:381721/149‑1 (MQ=255)                                                                                                                      ggCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACATGGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCa                          >  1:274833/1‑149 (MQ=255)                                                                                                                              tcgAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAg                  >  2:58942/1‑149 (MQ=255)                                                                                                                               cgAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGc                 >  2:163028/1‑149 (MQ=255)                                                                                                                                gagaAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGcc                >  5:188658/1‑149 (MQ=255)                                                                                                                                gagaAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGc                 <  5:248018/148‑1 (MQ=255)                                                                                                                                  gaAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGc              >  2:326186/1‑149 (MQ=255)                                                                                                                                     gCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGc           <  1:58942/149‑1 (MQ=255)                                                                                                                                     gCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGc           <  5:76288/149‑1 (MQ=255)                                                                                                                                           cGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGc     >  2:49804/1‑149 (MQ=255)                                                                                                                                           cGAAGTCGGTCCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGc     <  5:30373/149‑1 (MQ=255)                                                                                                                                           cGAAGTCGGTCCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGc     <  5:24946/149‑1 (MQ=255)                                                                                                                                           cGAAGGCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGc     <  7:256657/149‑1 (MQ=255)                                                                                                                                            gAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGCg    >  1:309035/1‑149 (MQ=255)                                                                                                                                              aGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGCGTg  >  2:131849/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                              ACCTGCAAGCGCTACGGCATGGTCAACGACGACATCCTTACCCCGGTGCAGAGCAACGGCGTGTATGTCGACTCGCTGCCATTCTTCGGCGGCCAGTTCATCTGGAAGGC‑CAACCCGGCCATCGTCGAGAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGCCACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGCTGCGTGAGCGTG  >  NC_002947/711223‑711511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑