Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	718,862	T→A	9.1%	E352V (GAA→GTA)	PP_0613 ←	amidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	718,862	0	T	A	9.1%	125.2 / 3.1	44	E352V (GAA→GTA)	PP_0613	amidase family protein
Reads supporting (aligned to +/- strand):  ref base T (23/17);  new base A (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CATCCCCGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTCCAGCGGGTCGCTGAAGCC  >  NC_002947/718720‑719010                                                                                                                                               |                                                                                                                                                     cATCCCCGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGcc                                                                                                                                                  >  1:237138/1‑147 (MQ=255)  aTCCCCGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAg                                                                                                                                               <  6:386577/149‑1 (MQ=255)     cccGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTTGGCGAATACACCCAGGc                                                                                                                                             >  8:292292/1‑148 (MQ=255)     cccGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTTGGCGAATACACCCAGGc                                                                                                                                             <  7:292292/148‑1 (MQ=255)      ccGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTTGGCGAATACACCCAGGcgc                                                                                                                                           >  7:246721/1‑147 (MQ=255)         aCCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTTGGCGAATACACCCAGGcgcttg                                                                                                                                        <  8:246721/149‑6 (MQ=255)          cccAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCt                                                                                                                                       <  7:313367/149‑1 (MQ=255)                cggcACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCAc                                                                                                                                 <  2:237138/149‑1 (MQ=255)                           gtCCGGCTTCGAACGCCCCCAACGGCAGCATCGGCAATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGc                                                                                                                       >  1:255267/1‑148 (MQ=255)                           gtCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGc                                                                                                                       >  7:316699/1‑148 (MQ=255)                            tCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCATGTTCGGCa                                                                                                                      >  3:37390/1‑148 (MQ=255)                              cGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATc                                                                                                                   >  5:261136/1‑149 (MQ=255)                                gCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCa                                                                                                                 <  5:21163/149‑1 (MQ=255)                                       aCGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGc                                                                                                          <  7:6759/149‑1 (MQ=255)                                           caccaACGGCAGCATCGGCGATACCCGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCg                                                                                                      >  3:331246/1‑149 (MQ=255)                                              caACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGt                                                                                                   <  8:33903/149‑1 (MQ=255)                                                 cGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCaa                                                                                                <  2:66949/149‑1 (MQ=255)                                                 cGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCaa                                                                                                <  8:316699/149‑1 (MQ=255)                                                 cGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAAATCTCCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCaa                                                                                                <  7:65416/149‑1 (MQ=255)                                                         tCGGCGCTACCAGCACGTCATATCGCTGATGAAAGGCATTCATGGTCGCAATCAATTCTGCGCGTGCCTGAAGAGCCTGGGGGTAGTCGCCAAGGCTGATCTGTGCAGGTGGTTCGGACATCCAGCGCAGCGCCGGGTCCAggacctct                                                                                        >  6:109841/1‑142 (MQ=255)                                                         tCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCt                                                                                        <  6:261136/149‑1 (MQ=255)                                                              gATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTg                                                                                   <  2:171911/149‑1 (MQ=255)                                                                  ccAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCa                                                                               >  3:113452/1‑149 (MQ=255)                                                                     gCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCgg                                                                                                     >  6:263661/1‑124 (MQ=255)                                                                             tatCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAgg                                                                     >  1:145684/1‑148 (MQ=255)                                                                                                   tCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGcgccgcgccg                                              >  8:79769/1‑149 (MQ=255)                                                                                                       cGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCa                                           >  5:3989/1‑148 (MQ=255)                                                                                                          aatcaatTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCa                                                                                             >  4:122369/1‑95 (MQ=255)                                                                                                          aatcaatTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCa                                                                                             <  3:122369/95‑1 (MQ=255)                                                                                                          aatcaatTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCCCCGCGCCGGCAAAc                                        >  3:28170/1‑148 (MQ=255)                                                                                                           atcaatTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACca                                      >  1:8957/1‑149 (MQ=255)                                                                                                            tcaatTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACcac                                     >  6:35690/1‑149 (MQ=255)                                                                                                               atTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAgg                                  <  6:3989/149‑1 (MQ=255)                                                                                                                ttCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAggg                                 >  5:140610/1‑149 (MQ=255)                                                                                                                  cTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGgtg                               >  7:218600/1‑149 (MQ=255)                                                                                                                   tGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGgtgt                              >  3:170153/1‑149 (MQ=255)                                                                                                                    gCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGtt                             >  3:141570/1‑149 (MQ=255)                                                                                                                     cccGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGgtg                               <  2:138621/146‑1 (MQ=255)                                                                                                                       cGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTg                            >  1:95059/1‑147 (MQ=255)                                                                                                                       cGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTg                            >  3:251241/1‑147 (MQ=255)                                                                                                                               aaGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTcc                   <  4:28170/148‑1 (MQ=255)                                                                                                                                        ggTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTCCAGCGGGTc           <  2:145684/147‑1 (MQ=255)                                                                                                                                            tATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCCGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTCCAGCGGGTCGCTg       >  7:13801/1‑147 (MQ=255)                                                                                                                                            tATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCATCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTCCAGCGGGTCGCTGaa     <  2:255267/149‑1 (MQ=255)                                                                                                                                               tCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTCCAGCGGGTCGCTGAAGcc  >  6:382547/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                     CATCCCCGACCCAGGCGGCACGTTGTGTCCGGCTTCGAACGCCACCAACGGCAGCATCGGCGATACCAGCACGTCATATCGCTGATGAAAGGCATTCATCTTCGCAATCAATTCTGCCCGTGCCTCAAGCGCCTGGGTGTATTCGCCAAGGCTGATCTGTGCACCTTGTTCGGCAATCCAGCGCAGCCCCGGGTCAAGCAACGCTTTCTGCTCATCGCTCAAGGCACTGGCCAACCGCGCCGCGCCGGCAAACCACAGGGTGTTGAAGGTTTCCAGCGGGTCGCTGAAGCC  >  NC_002947/718720‑719010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑