Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 727,316 A→T 6.9% W6R (TGG→AGG)  PP_0621 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947727,3160AT 6.9% 193.9 / 3.1 67W6R (TGG→AGG) PP_0621hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/28);  new base T (3/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGC  >  NC_002947/727176‑727459
                                                                                                                                            |                                                                                                                                               
ctggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCTAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTcc                                                                                                                                         <  3:299182/149‑1 (MQ=255)
ctggctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTcc                                                                                                                                         <  8:1524/149‑1 (MQ=255)
   gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTccc                                                                                                                                        <  4:183745/147‑1 (MQ=255)
   gctgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCTTGGGCCTccc                                                                                                                                        >  3:183745/1‑147 (MQ=255)
     tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                     >  4:29326/1‑148 (MQ=255)
     tgACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCttt                                                                                                                                     >  2:279233/1‑148 (MQ=255)
       aCTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATAAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAACCCAACAGTCTAGGACTCGGCCAATAAAAAAGGGAGGCACAAGGCCTCCCTttca                                                                                                                                   >  4:316295/1‑148 (MQ=255)
         tttGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTttcatt                                                                                                                                 >  5:247070/1‑148 (MQ=255)
             tATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCg                                                                                                                            >  3:282701/1‑149 (MQ=255)
             tATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCg                                                                                                                            >  3:277639/1‑149 (MQ=255)
                  gctccACAGCCAGAACGCTGCTGCGATCAGGGCGATCCAGAAAAGTAGGCCAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGa                                                                                                                        <  8:248627/145‑1 (MQ=255)
                   cgcCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGaa                                                                                                                       <  1:282983/148‑1 (MQ=255)
                       acaGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCGAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCGCCCTTTCATTCACCGGTGAAGGt                                                                                                                    >  8:100112/1‑147 (MQ=255)
                       acaGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCa                                                                                                                  <  6:101534/149‑1 (MQ=255)
                         aGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGCGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGACAGt                                                                                                                >  5:127063/1‑149 (MQ=255)
                         aGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGt                                                                                                                >  7:186693/1‑149 (MQ=255)
                            cAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCg                                                                                                              >  7:17647/1‑148 (MQ=255)
                            cAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCg                                                                                                              >  7:374/1‑148 (MQ=255)
                              gAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGaa                                                                                                            >  8:204467/1‑148 (MQ=255)
                                aCGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAg                                                                                                         <  4:282701/149‑1 (MQ=255)
                                aCGAGGAGGCTATAAGTGCGAGACAGAAAAGTAGTCGAAACATGTTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAAAAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCTAACAg                                                                                                         <  4:277639/149‑1 (MQ=255)
                                      cggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCaaa                                                                                                   <  8:186693/149‑1 (MQ=255)
                                      cggcgATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCaaa                                                                                                   <  3:253509/149‑1 (MQ=255)
                                           aTCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTCTCAAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACACCTTAAGCTCAGTCGAACAAACCAAACGTc                                                                                               >  4:51140/1‑148 (MQ=255)
                                              aGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATg                                                                                            <  1:369130/148‑1 (MQ=255)
                                                 gcgcTCCATAAAATGAGGGGAACCATGGTGTCCAGGTTGTCGTTTGAACAAGCCAACAGTCTAGGACGCGGGCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAg                                                                                         <  6:127063/148‑1 (MQ=255)
                                                      ccAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAAc                                                                                   <  3:29326/149‑1 (MQ=255)
                                                        aGAAAAGTAGGCGAACCATTGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCa                                                                                 <  5:38623/149‑1 (MQ=255)
                                                        aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAg                                                                                                                      <  6:204038/112‑1 (MQ=255)
                                                        aGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAg                                                                                                                      >  5:204038/1‑112 (MQ=255)
                                                         taaaaGTAGGCTAACCATGGTGTCCAGCTTGTCGATTGAATAATCCAACAGTCTATGACGCGGCCAATAAAAATGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                <  3:316295/148‑1 (MQ=255)
                                                         gAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAg                                                                                >  1:240930/1‑149 (MQ=255)
                                                              gTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACg                                                                           <  1:279233/149‑1 (MQ=255)
                                                                    gAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCttt                                                                     >  2:140088/1‑149 (MQ=255)
                                                                       ccATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTtctc                                                                  >  7:28119/1‑149 (MQ=255)
                                                                                 cAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTc                                                        >  5:334323/1‑149 (MQ=255)
                                                                                   gCTTGTCGTTTGAAGTAGCGAACAGTCTAGGACGCGGCCAATAAAAAATCGAGGCGCTAGGCCTCACTTTCATTGTCCTCTGAAGGTCAGTCGAACAGACCAAAGGTCATTTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTctg                                                      <  8:374/149‑3 (MQ=255)
                                                                                   gCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                      >  8:116636/1‑149 (MQ=255)
                                                                                   gCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCgg                                                      <  7:343087/149‑1 (MQ=255)
                                                                                               aagaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCt                                                                       >  1:229014/1‑120 (MQ=255)
                                                                                               aagaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCt                                                                       <  2:229014/120‑1 (MQ=255)
                                                                                               aagaagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCa                                           >  5:278169/1‑148 (MQ=255)
                                                                                                 gaagCCAACAGTCTAGGACGCGGCCAATAAAAACGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCGGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCAc                                          >  2:180335/1‑147 (MQ=255)
                                                                                                  aagCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGAt                                       <  6:247070/149‑1 (MQ=255)
                                                                                                       aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCt                                                                                                                                           <  8:188629/44‑1 (MQ=255)
                                                                                                       aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCt                                                                                                                                           >  7:188629/1‑44 (MQ=255)
                                                                                                       aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGt                                  >  4:115979/1‑149 (MQ=255)
                                                                                                       aaCAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGt                                  >  7:348874/1‑149 (MQ=255)
                                                                                                           gTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGcc                              <  7:116636/149‑1 (MQ=255)
                                                                                                              tAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGtt                           >  4:335710/1‑149 (MQ=255)
                                                                                                               aGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGaa                                                                             >  5:315760/1‑98 (MQ=255)
                                                                                                               aGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGaa                                                                             <  6:315760/98‑1 (MQ=255)
                                                                                                                 gACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTtctc                        <  1:140088/149‑1 (MQ=255)
                                                                                                                   cgcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTGGt                      <  2:240930/149‑1 (MQ=255)
                                                                                                                    gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTcc                                               <  2:387739/123‑1 (MQ=255)
                                                                                                                    gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTcc                                               >  1:387739/1‑123 (MQ=255)
                                                                                                                    gcgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGCCCTTCCGGCACGAGTGGGTCGACGTGCACGTc                     >  6:266752/1‑149 (MQ=255)
                                                                                                                     cgGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCt                    <  8:348874/149‑1 (MQ=255)
                                                                                                                            tAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAgg               >  8:236818/1‑147 (MQ=255)
                                                                                                                              aaaaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGgcag            >  2:70926/1‑148 (MQ=255)
                                                                                                                                aaaGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGcagcag         >  5:88060/1‑149 (MQ=255)
                                                                                                                                 aaGGGAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCagcagc        >  4:21139/1‑149 (MQ=255)
                                                                                                                                  aGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGct       >  7:299287/1‑149 (MQ=255)
                                                                                                                                   gggAGGCCTTGGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGctc      >  6:59368/1‑149 (MQ=255)
                                                                                                                                      aGGCCCAAGGCCTCCCTTACATTCACCGCTGAAGGTCAGTCCAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCTTTCTCGTCTTTAGCCAGCAGCtctcc   >  2:256950/1‑148 (MQ=255)
                                                                                                                                       ggCCCAAGGCGTCCCTTTCATTCTCCGCTGTAGGTCAGTCGAACAGACCTAAGGTGATGAAGCTGTACCAGGAACGGTCTTTCTCGGGTTCTTTCCGGCGTTCCGGCACGATTTGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTcgc  <  3:51140/149‑1 (MQ=255)
                                                                                                                                       ggCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTcgc  <  7:204467/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                               
CTGGCTGACTTTGTATTTGCGCCACAGCCAGAACGCGGCGGCGATCAGGGCGATCCAGAAAAGTAGGCGAACCATGGTGTCCAGCTTGTCGATTGAAGAAGCCAACAGTCTAGGACGCGGCCAATAAAAAAGGGAGGCCCAAGGCCTCCCTTTCATTCACCGCTGAAGGTCAGTCGAACAGACCAAAGGTCATGTAGCTGAACCAGGAACGGTCTTTCTCGGCTTCTTTCGGGCCTTCCGGCACGATTGGGTCGCCGTTCTCGTCTTTAGGCAGCAGCTCTCGC  >  NC_002947/727176‑727459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑