Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	760,824	G→C	6.7%	P239R (CCC→CGC) ‡	PP_0652 ←	gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	760,824	0	G	C	6.7%	239.7 / 5.1	75	P239R (CCC→CGC) ‡	PP_0652	gluconate transporter
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base C (2/3);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

GCCCAGCAGCATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAGGAAGCCA  >  NC_002947/760676‑760967                                                                                                                                                     |                                                                                                                                                 gCCCAGCAGCATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcgg                                                                                                                                                  >  6:169631/1‑149 (MQ=255)   ccAGCAGCATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcgggg                                                                                                                                                >  6:239955/1‑149 (MQ=255)   ccAGCAGCATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcgggg                                                                                                                                                >  7:148/1‑149 (MQ=255)   ccAGCAGCATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcgggg                                                                                                                                                >  7:19636/1‑149 (MQ=255)     ccgattctGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcgggg                                                                                                                                                <  5:207732/140‑1 (MQ=255)     agcagcATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGACGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcggggcg                                                                                                                                              <  7:291910/149‑1 (MQ=255)       cagcaTGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcggggcggg                                                                                                                                            <  3:229841/149‑1 (MQ=255)        agcaTGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCcggggcgggg                                                                                                                                           >  8:278346/1‑149 (MQ=255)            tGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTgg                                                                                                                                       <  4:363135/149‑1 (MQ=255)            tGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGC‑GGGTTGGc                                                                                                                                      >  5:239190/1‑149 (MQ=255)            tGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACACCGGGGC‑GGGTTGGc                                                                                                                                      >  5:233739/1‑149 (MQ=255)              gCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGCCCCGGGGtaatg                                                                                                                                      <  7:128587/148‑5 (MQ=255)                 aTCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATc                                                                                                                                  <  6:159484/149‑1 (MQ=255)                 aTCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGCCCCGGGGtaatggt                                                                                                                                    <  5:292038/147‑7 (MQ=255)                 aTCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGCCCCGGGGtaatggt                                                                                                                                    >  6:292038/1‑141 (MQ=255)                    aGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGc                                                                                                                               <  3:13351/149‑1 (MQ=255)                     gCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCt                                                                                                                              >  6:264564/1‑149 (MQ=255)                        ggTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCtttt                                                                                                                           <  7:126779/149‑1 (MQ=255)                        ggTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCtttt                                                                                                                           <  2:131026/149‑1 (MQ=255)                             cccAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGc                                                                                                                      <  5:143732/149‑1 (MQ=255)                             cccAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGc                                                                                                                      >  7:153984/1‑149 (MQ=255)                              ccAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCAAGCACCACCATCAGGATGGACGGCATAAATGCGGCCAGCATGCCGACCATTACCCAGGGGAGCGGTTGGCCATCGGCTTTTTCAGc                                                                                                                      >  8:133434/1‑148 (MQ=255)                               cAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCa                                                                                                                    <  2:217173/149‑1 (MQ=255)                               cAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGAAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCa                                                                                                                    <  8:153984/149‑1 (MQ=255)                               cAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGAGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGc                                                                                                                      >  1:264259/1‑147 (MQ=255)                                aGGAAGCTGGCCATCTCCGGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAg                                                                                                                   >  7:30905/1‑149 (MQ=255)                                aGGAAGCTGGCCATCTCCGGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAg                                                                                                                   <  8:30905/149‑1 (MQ=255)                                  gAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAg‑tg                                                                                                                >  5:297629/1‑149 (MQ=255)                                   aaGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAg‑tgt                                                                                                               <  2:289527/149‑1 (MQ=255)                                       tGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAAc                                                                                                           >  1:268958/1‑149 (MQ=255)                                         gCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAAc                                                                                                           <  2:328112/147‑1 (MQ=255)                                         gCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTg                                                                                                         <  7:298596/149‑1 (MQ=255)                                            aTCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTc                                                                                                      <  8:148/149‑1 (MQ=255)                                            aTCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTc                                                                                                      <  8:329872/149‑1 (MQ=255)                                            aTCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGCCCCGGGGtaatg                                                                                                                                      <  2:96619/118‑5 (MQ=255)                                            aTCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGCCCCGGGGtaatg                                                                                                                                      >  1:96619/1‑114 (MQ=255)                                               tcCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGAGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAg                                                                                                   >  3:176047/1‑149 (MQ=255)                                                ccAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAg                                                                                                   >  3:350039/1‑148 (MQ=255)                                                 cagcagCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTc                                                                                                      <  3:379323/144‑1 (MQ=255)                                                 cagcagCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTc                                                                                                      >  4:379323/1‑144 (MQ=255)                                                 cagcagCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCa                                                                                                    <  5:264564/146‑1 (MQ=255)                                                 cagcagCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAg                                                                                                   <  2:69745/147‑1 (MQ=255)                                                  agcagcGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCaa                                                                                                <  4:71612/149‑1 (MQ=255)                                                      gcgcATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGcc                                                                                            <  5:239955/149‑1 (MQ=255)                                                       cgcATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGACCTGGTCCAGCAATGCCt                                                                                           >  3:293618/1‑149 (MQ=255)                                                                   gggCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCcgc                                                                                <  5:169631/148‑1 (MQ=255)                                                                    ggCAGCAGCATCTCGGCGACGGCAAGCTCCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCgt                                                                              <  7:133434/149‑1 (MQ=255)                                                                       agcagcaTCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGtt                                                                           <  4:337093/149‑1 (MQ=255)                                                                          agcaTCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCAt                                                                        >  5:178525/1‑149 (MQ=255)                                                                             aTCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCa                                                                                                    <  7:359038/118‑1 (MQ=255)                                                                             aTCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCa                                                                                                    >  8:359038/1‑118 (MQ=255)                                                                                tcGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTg                                                                                                         >  3:245028/1‑110 (MQ=255)                                                                                tcGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTg                                                                                                         <  4:245028/110‑1 (MQ=255)                                                                                tcGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCggg                                                                                    <  1:241497/131‑1 (MQ=255)                                                                                tcGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCggg                                                                                    >  2:241497/1‑131 (MQ=255)                                                                                tcGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCgg                                                                  >  3:229894/1‑149 (MQ=255)                                                                                  ggCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCgg                                                                  <  8:19636/147‑1 (MQ=255)                                                                                   gCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTg                                                                >  2:282797/1‑148 (MQ=255)                                                                                    cGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGa                                                               >  3:155639/1‑148 (MQ=255)                                                                                     gACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGAGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGACGCGTGTTCATGCGCGGTTaaa                                                             >  5:272147/1‑149 (MQ=255)                                                                                         gCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGaa                                                         <  2:112961/149‑1 (MQ=255)                                                                                              cACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATg                                                     <  7:278346/148‑1 (MQ=255)                                                                                               aCCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGcc                                                   <  4:229894/149‑1 (MQ=255)                                                                                                 cAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATg                                                     >  6:26751/1‑145 (MQ=255)                                                                                                 cAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATg                                                     <  5:26751/145‑1 (MQ=255)                                                                                                        aGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCCGCGCGGGGTTGGCCATAGGCTTTTTCAGCCAC‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCAAGCGCGGTGAAATGAACATGCACTACAGCgg                                          >  5:64343/1‑149 (MQ=255)                                                                                                          gATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGgc                                        >  6:236200/1‑149 (MQ=255)                                                                                                             ggCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCggg                                         <  1:15359/145‑1 (MQ=255)                                                                                                             ggCCGGCAGCAATGCGGCCAGCATGCAGACCATTACACCGGGGCGGGGTTGGACATCGGCTTTTTCAGACAG‑TGTGAACTGGACCAGCAAAGCCTCATCCGGCCGCGTGTTCATGCTCGGTGAAATGAACATGCCGTACATCGGgcag                                      >  2:15359/1‑146 (MQ=255)                                                                                                               ccGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGATCATGCGCGGTGAAATGAACATGCCGTACAGCGGgccgcc                                    >  3:323479/1‑148 (MQ=255)                                                                                                                  gcagcaATGCGGCCAGCATGCCGACCATTACCCCGGGGC‑GGGTTGTCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTTAAATGAACATGCCGTACAGCGGGCCGCCCAGGa                               <  6:239190/149‑1 (MQ=255)                                                                                                                  gcagcaATGCGGCCAGCATGCCGACCATTACCCCGGGGC‑GGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTTAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGa                               <  6:233739/149‑1 (MQ=255)                                                                                                                   cagcaATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGa                               <  6:297629/149‑1 (MQ=255)                                                                                                                           cGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAGTTATGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGGGCGGTGAAATGAACATGCCGTACAGCGGCCCGCCCAGGATCATTGc                        >  1:305357/1‑149 (MQ=255)                                                                                                                            ggCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCg                      <  3:222482/149‑1 (MQ=255)                                                                                                                             gCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCg                      >  3:202483/1‑148 (MQ=255)                                                                                                                                  cATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATgg                >  4:64065/1‑149 (MQ=255)                                                                                                                                         aCCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAg         >  2:63186/1‑149 (MQ=255)                                                                                                                                           cATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAgg        >  2:310485/1‑148 (MQ=255)                                                                                                                                             ttACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAGGAAg     >  7:298305/1‑149 (MQ=255)                                                                                                                                                ccccGGGGCGGGGTTGGCCATCGGCTGTTTCTGGCAG‑TGTGAACTTGTCCAGCAATGCCTGATCGGGCCGCGTGTTCTTGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGGAAGGTAGcga  <  6:64343/149‑3 (MQ=255)                                                                                                                                                 cccGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAGGAAGCCa  >  8:151567/1‑148 (MQ=255)                                                                                                                                                 cccGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAGGAAGCCa  >  1:167021/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GCCCAGCAGCATGGCGATCAGCGGGTTGCCCAGGAAGCTGGCCATCTCCAGCAGCGCATTGCCTTTGGGCAGCAGCATCTCGGCGACGGCATGCACCAGCATCAGGATGGCCGGCAGCAATGCGGCCAGCATGCCGACCATTACCCCGGGGCGGGGTTGGCCATCGGCTTTTTCAGCCAG‑TGTGAACTGGTCCAGCAATGCCTGATCGGGCCGCGTGTTCATGCGCGGTGAAATGAACATGCCGTACAGCGGGCCGCCCAGGATCATTGCCGGAATGGCGGCAAGGAAGCCA  >  NC_002947/760676‑760967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑