Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	762,209	G→T	6.2%	V81L (GTG→TTG)	mdh →	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	762,209	0	G	T	6.2%	208.6 / 3.6	64	V81L (GTG→TTG)	mdh	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base T (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

ATGGACGTGCAGGGTGAGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTCCAACCCGGTCGATGTGCTCACCTACCGCGC  >  NC_002947/762065‑762357                                                                                                                                                  |                                                                                                                                                     aTGGACGTGCAGGGTGAGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGcc                                                                                                                                                  >  2:73260/1‑149 (MQ=255) aTGGACGTGCAGGGTGAGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGcc                                                                                                                                                  >  2:146210/1‑149 (MQ=255)   ggACGTGCAGGGTGAGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGGCCGGGCTTGcgc                                                                                                                                                 >  7:343475/1‑147 (MQ=255)         gCAGGGTGAGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGc                                                                                                                                          >  2:167169/1‑149 (MQ=255)                 aGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCa                                                                                                                                   <  1:73260/148‑1 (MQ=255)                 aGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCa                                                                                                                                   <  2:334708/148‑1 (MQ=255)                  gCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGGCCGGGCTTGCGCGGCACGCCCgccgtg                                                                                                                                  <  8:343475/149‑6 (MQ=255)                   cTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTc                                                                                                                                >  1:353195/1‑149 (MQ=255)                   cTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTc                                                                                                                                >  1:104806/1‑149 (MQ=255)                   cTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCAGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTc                                                                                                                                >  3:153083/1‑149 (MQ=255)                        gcAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCgcgc                                                                                                                            >  4:76713/1‑148 (MQ=255)                             gCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGAt                                                                                                                      <  4:339161/149‑1 (MQ=255)                              cAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTc                                                                                                                                <  5:70403/138‑1 (MQ=255)                              cAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTc                                                                                                                                >  6:70403/1‑138 (MQ=255)                                    gcTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGa                                                                                                               <  3:244547/149‑1 (MQ=255)                                        gACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTAc                                                                                                           <  5:48713/149‑1 (MQ=255)                                           gTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATTCCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCaa                                                                                                        <  2:353195/149‑1 (MQ=255)                                           gTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCaa                                                                                                        >  5:221265/1‑149 (MQ=255)                                            gttGGCAGGCGGCTGCGGATTCCGGGTCCGCTACCCATGTTCACGTCGGGTCGAATGGCGAGATGCTGGTGGGTTCCGATCTGGTGGTGATCTCGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTGGCTGCATGATGTATTGAGttcgtag                                                                                                       <  8:346657/148‑7 (MQ=255)                                               ggCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGGGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCt                                                                                                     <  2:335929/148‑1 (MQ=255)                                                gCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGc                                                                                                   >  7:118991/1‑149 (MQ=255)                                                gCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGc                                                                                                   <  4:153083/149‑1 (MQ=255)                                                     cggcTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTTGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATc                                                                                              <  6:16963/149‑1 (MQ=255)                                                          gtgGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGa                                                                                                               >  3:228945/1‑127 (MQ=255)                                                          gtgGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGa                                                                                                               <  4:228945/127‑1 (MQ=255)                                                          gtgGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAAc                                                                                                       >  7:346893/1‑135 (MQ=255)                                                           tgGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGa                                                                                         <  2:176663/148‑1 (MQ=255)                                                              aTTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAg                                                                                      >  5:387517/1‑148 (MQ=255)                                                               ttCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTAt                                                                                                                  <  1:269336/119‑1 (MQ=255)                                                               ttCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTAt                                                                                                                  >  2:269336/1‑119 (MQ=255)                                                               ttCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGC‑CGGGCTTGCGCGGCACGCCCgcc                                                                                                                                     <  6:388225/100‑3 (MQ=255)                                                               ttCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGC‑CGGGCTTGCGCGGCACGCCCgcc                                                                                                                                     >  5:388225/1‑98 (MQ=255)                                                               gtCACGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGAGGTATTGAg                                                                                                              <  8:296015/122‑1 (MQ=255)                                                                  aGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGcatc                                                                                  <  6:387517/148‑1 (MQ=255)                                                                    gTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCt                                                                                            <  5:14027/136‑1 (MQ=255)                                                                    gTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCt                                                                                            >  6:14027/1‑136 (MQ=255)                                                                       ccGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGc                                                                             <  3:76713/148‑1 (MQ=255)                                                                        cGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCg                                                                            <  6:184690/148‑1 (MQ=255)                                                                         gATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGa                                                                          >  4:375068/1‑149 (MQ=255)                                                                         gATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGa                                                                          <  8:118991/149‑1 (MQ=255)                                                                         gATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGT‑‑CGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGata                                                                        >  6:74410/1‑149 (MQ=255)                                                                             cccATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATc                                                                      <  7:249413/149‑1 (MQ=255)                                                                              tcATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTAt                                                                                                                  >  2:330831/2‑104 (MQ=255)                                                                              tcATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTAt                                                                                                                  <  1:330831/103‑1 (MQ=255)                                                                              ccATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCg                                                                            <  4:145114/142‑1 (MQ=255)                                                                              ccATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCg                                                                            >  3:145114/1‑142 (MQ=255)                                                                              ccATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATc                                                                      >  2:113590/1‑148 (MQ=255)                                                                              ccATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATc                                                                      >  8:153775/1‑148 (MQ=255)                                                                              ccATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATc                                                                      >  2:110201/1‑148 (MQ=255)                                                                                aTGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAg                                                                   >  5:345656/1‑149 (MQ=255)                                                                                   ttCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGcac                                                                >  5:311273/1‑149 (MQ=255)                                                                                     cACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGcacca                                                              >  1:175558/1‑149 (MQ=255)                                                                                           ggggCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGT‑‑CGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGa                                                      <  5:74410/149‑1 (MQ=255)                                                                                                      cGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACgg                                             >  4:367168/1‑149 (MQ=255)                                                                                                            gCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCtgg                                       >  5:207829/1‑149 (MQ=255)                                                                                                                gAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCtggtg                                     <  1:146210/147‑1 (MQ=255)                                                                                                                gAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCtggtg                                     <  2:175558/147‑1 (MQ=255)                                                                                                                     ttCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAACATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTcc                               >  4:178094/1‑148 (MQ=255)                                                                                                                           gCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCtg                                        >  6:172818/1‑133 (MQ=255)                                                                                                                           gCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCtg                                        <  5:172818/133‑1 (MQ=255)                                                                                                                             tggtggTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTCCAACCCGGTc                      >  1:213071/1‑149 (MQ=255)                                                                                                                                tggtgATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTCCAACCCGGTCGAt                   <  1:113590/149‑1 (MQ=255)                                                                                                                                      tCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGCCAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGGCCAACCCGGTCGATGTGCTc             >  4:298881/1‑149 (MQ=255)                                                                                                                                      tCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTCCAACCCGGTCGATGTGCt              >  3:171205/1‑148 (MQ=255)                                                                                                                                                  gTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTCCAACCCGGTCGATGTGCTCACCTACCgcgc  <  6:207829/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     ATGGACGTGCAGGGTGAGCTGGCGCAGGGCAAGGCGCTGGACGTTTGGCAGGCGGCTGTGGATTCAGGTTCCGATACCCATGTTCACGGCGGGGCCAAAGCCGAGATGCTGGAGGGTTCCGAGCTGGTGGTGATCACGG‑CGGGCGTGCCGCGCAAGCCCGGCCAGTCGCGCCAGGATGTATTGAGTACCAACCTGCCAATCCTCGACAGCATCATGGCGGATATCAAGCACCACGCGCCGACTGCGACGGTGCTGGTGGTGTCCAACCCGGTCGATGTGCTCACCTACCGCGC  >  NC_002947/762065‑762357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑