Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	776,568	T→C	6.5%	M225T (ATG→ACG)	PP_0668 →	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	776,568	0	T	C	6.5%	179.6 / 2.4	61	M225T (ATG→ACG)	PP_0668	transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GTGACGAGGGCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCGCGATTACCGCCATGGCCACTTG  >  NC_002947/776420‑776713                                                                                                                                                     |                                                                                                                                                  gtgACGAGGGCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACAACGTTGCAAGCCGGGCAAat                                                                                                                                                   >  4:30592/1‑149 (MQ=255)   gACGAGGGCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAatga                                                                                                                                                 >  5:77810/1‑149 (MQ=255)     cGAGGGCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAatgatg                                                                                                                                               <  2:275962/149‑1 (MQ=255)     cGAGGGCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAatgatg                                                                                                                                               <  8:117366/149‑1 (MQ=255)         ggCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGgtgt                                                                                                                                           <  6:392555/149‑1 (MQ=255)          gCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGtt                                                                                                                                          <  4:306898/149‑1 (MQ=255)              ggCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCAGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGAc                                                                                                                                      <  2:185382/149‑1 (MQ=255)                   gTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGAcgcg                                                                                                                                   >  4:161406/1‑147 (MQ=255)                       aaCCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCgcagc                                                                                                                               >  2:239487/1‑147 (MQ=255)                          cGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTc                                                                                                                          <  4:160238/149‑1 (MQ=255)                               ggCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGgcg                                                                                                                      <  5:218237/148‑1 (MQ=255)                               ggCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGgc                                                                                                                       <  5:318511/147‑1 (MQ=255)                                 cACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGccc                                                                                                                   >  6:103847/1‑149 (MQ=255)                                        ttCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGa                                                                                                                                       >  8:218372/1‑122 (MQ=255)                                        ttCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGa                                                                                                                                       <  7:218372/122‑1 (MQ=255)                                        ttCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCGGCAAGTCGGCGCCCCATCAAc                                                                                                            >  5:337994/1‑149 (MQ=255)                                        ttCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAAc                                                                                                            >  5:90872/1‑149 (MQ=255)                                        ttCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAAc                                                                                                            >  1:144340/1‑149 (MQ=255)                                          cAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAAc                                                                                                            <  5:235354/147‑1 (MQ=255)                                          cAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACt                                                                                                           <  8:106955/148‑1 (MQ=255)                                          cAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTg                                                                                                          >  7:15162/1‑149 (MQ=255)                                             tgtCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAACGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGca                                                                                                        >  4:341883/1‑148 (MQ=255)                                             tgtCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAACGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGca                                                                                                        >  4:336446/1‑148 (MQ=255)                                               tCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAatgatg                                                                                                                                               <  1:19770/107‑1 (MQ=255)                                               tCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAatgatg                                                                                                                                               >  2:19770/1‑107 (MQ=255)                                               tCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACaa                                                                                                     >  2:84938/1‑149 (MQ=255)                                                    gACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCtt                                                                                                <  7:164385/149‑1 (MQ=255)                                                      ccAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGcc                                                                                                                    <  3:199953/127‑1 (MQ=255)                                                      ccAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGcc                                                                                                                    >  4:199953/1‑127 (MQ=255)                                                      ccAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTgc                                                                                              >  3:285662/1‑149 (MQ=255)                                                       cAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTgcg                                                                                             <  4:285662/149‑1 (MQ=255)                                                       cAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTgcg                                                                                             >  3:343801/1‑149 (MQ=255)                                                       cAGGGGTTGCAGACCACCCTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTgcg                                                                                             >  1:215830/1‑149 (MQ=255)                                                          gggTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTgcgc                                                                                            >  1:227388/1‑147 (MQ=255)                                                            gTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAg                                                                                         >  7:113636/1‑148 (MQ=255)                                                                cAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCCGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTg                                                                                    >  8:87966/1‑149 (MQ=255)                                                                cAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGAtt                                                                                     <  2:227388/148‑1 (MQ=255)                                                                        cTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCcgcg                                                                            >  5:121689/1‑149 (MQ=255)                                                                              cGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGgt                                                                          >  5:11818/1‑145 (MQ=255)                                                                              cGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGgt                                                                          <  6:11818/145‑1 (MQ=255)                                                                                tATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCGGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTtg                                                                    <  6:337994/149‑1 (MQ=255)                                                                                tATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTtg                                                                    <  2:196895/149‑1 (MQ=255)                                                                                  tttGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTtgg                                                                   <  1:340199/148‑1 (MQ=255)                                                                                     gCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGc                                                               >  3:310593/1‑149 (MQ=255)                                                                                      ccGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCg                                                              <  6:77810/149‑1 (MQ=255)                                                                                         gcgcAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCggag                                                           <  1:84938/149‑1 (MQ=255)                                                                                          cgcAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCggagg                                                          <  2:144340/149‑1 (MQ=255)                                                                                                 cAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGcc                                                   >  6:333216/1‑149 (MQ=255)                                                                                                     cAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGTCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGTATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTg                                               <  8:113636/149‑1 (MQ=255)                                                                                                     cAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTg                                               <  5:103847/149‑1 (MQ=255)                                                                                                      aaCTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTgg                                              <  3:161406/149‑1 (MQ=255)                                                                                                      aaCTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTgg                                              >  3:170605/1‑149 (MQ=255)                                                                                                      aaCTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAACGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTgg                                              <  3:336446/149‑1 (MQ=255)                                                                                                      aaCTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAACGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTgg                                              <  3:341883/149‑1 (MQ=255)                                                                                                                ggcagcggcagACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAgatgat                                    <  8:15162/149‑1 (MQ=255)                                                                                                                ggcagcggcagACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAgatgat                                    >  2:23959/1‑149 (MQ=255)                                                                                                                 gcagcggcagACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATc                                   <  3:30592/149‑1 (MQ=255)                                                                                                                 gcagcggcagACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATc                                   <  4:343801/149‑1 (MQ=255)                                                                                                                         agACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCt                           >  3:168142/1‑149 (MQ=255)                                                                                                                            cAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTgcg                        <  4:310593/149‑1 (MQ=255)                                                                                                                            cAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTgcg                        <  6:90872/149‑1 (MQ=255)                                                                                                                             aaaCCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTgcgc                       >  1:149821/1‑149 (MQ=255)                                                                                                                                     ttGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCTCGATTACCg               >  6:121289/1‑149 (MQ=255)                                                                                                                                         aaGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCGCGATTACCGCCAt           <  1:239487/149‑1 (MQ=255)                                                                                                                                           gCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCGCGATTACCGCCATgg         >  7:26291/1‑149 (MQ=255)                                                                                                                                                gCAAATGATGGGGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCGCGATTACCGCCATGGCCACt    >  4:55645/1‑149 (MQ=255)                                                                                                                                                  aaaTGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCGCGATTACCGCCATGGCCACTTg  >  7:115379/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GTGACGAGGGCTGGGCGGGTGCAACCGCTCGGCACGCGCTTCAGTGTCCGGGACCAGGGGTTGCAGACCACCTTGAGCGTATTTGCCGGCGCAGTGCAGGCAACTTGCTCCGGCAGCGGCAGACAAACCACGTTGCAAGCCGGGCAAATGATGGTGTTCGACGCGCAGCAAGTCGGCGCCCCATCAACTGCACAACGCTTGCGCGAGGATTGGAGCCGCGGTGTGTTGGTGGCGGAGGACATGCCGCTGGTTCAGATGATCGACATTCTGCGCGATTACCGCCATGGCCACTTG  >  NC_002947/776420‑776713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑