Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	812,319	C→A	9.5%	intergenic (‑68/‑160)	PP_0698 ← / → PP_0699	LysR family transcriptional regulator/LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	812,319	0	C	A	9.5%	150.7 / 7.2	53	intergenic (‑68/‑160)	PP_0698/PP_0699	LysR family transcriptional regulator/LysE family transporter
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base A (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.49e-01

GTGCTCGGCGGCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATC  >  NC_002947/812171‑812465                                                                                                                                                         |                                                                                                                                                   gTGCTCGGCGGCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTccc                                                                                                                                                    <  6:378810/149‑1 (MQ=255)  tGCTCGGCGGCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCt                                                                                                                                                   <  5:334718/149‑1 (MQ=255)     tCGGCGGCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTg                                                                                                                                                  >  7:226198/1‑147 (MQ=255)            cgcgcACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAg                                                                                                                                         <  7:292546/149‑1 (MQ=255)             gcgcACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAgg                                                                                                                                        <  4:251998/149‑1 (MQ=255)                cACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGttt                                                                                                                                     <  2:133762/149‑1 (MQ=255)                  cGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTAc                                                                                                                                   <  8:39294/149‑1 (MQ=255)                  cGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTAc                                                                                                                                   <  4:163071/149‑1 (MQ=255)                   gAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTAcc                                                                                                                                  >  4:120400/1‑149 (MQ=255)                     aGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAGCCTGCCTT‑‑GGCCTCTTCGCGGG‑TAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGTTTAccc                                                                                                                                 <  5:226497/149‑1 (MQ=14)                      gCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCCTTGGCCTCTTCGCGGG‑TAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGTTTAccc                                                                                                                                 <  7:276700/149‑1 (MQ=17)                       ctctCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCcgc                                                                                                                               >  4:29174/1‑148 (MQ=255)                           cGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAaga                                                                                                                          <  8:226198/149‑1 (MQ=255)                             gTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGttt                                                                                                                                     >  3:117912/1‑136 (MQ=18)                             gTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGttt                                                                                                                                     <  4:117912/136‑1 (MQ=18)                             gTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAgg                                                                                                                        >  3:225690/1‑149 (MQ=255)                                    cggcAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATCATATCCCTGTTCAG‑CTGGGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAaggc                                                                                                                 <  5:52943/149‑1 (MQ=255)                                      gcAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCGCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAaggcag                                                                                                               <  7:249776/149‑1 (MQ=255)                                         aTGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCt                                                                                                            <  5:78098/149‑1 (MQ=255)                                           gTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCCGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACACGCGAAGAGGCCAAGGCAGGCTg                                                                                                           >  7:161645/1‑148 (MQ=255)                                                aGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACata                                                                                                     >  7:359390/1‑149 (MQ=255)                                                  tAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATc                                                                                                   >  7:83132/1‑149 (MQ=255)                                                     cGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCtgt                                                                                                <  5:145253/149‑1 (MQ=255)                                                     cGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCtgt                                                                                                <  3:120400/149‑1 (MQ=255)                                                           gCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCa                                                                                           >  7:117529/1‑148 (MQ=255)                                                             gTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGGCTAAACCCGCTCCTACATGGCCACATATTTCCCTGTa                                                                                                                                                <  1:64585/94‑1 (MQ=255)                                                             gTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTa                                                                                                                                                >  2:64585/1‑93 (MQ=255)                                                                gAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTa                                                                                     >  7:67997/1‑149 (MQ=255)                                                                  gTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATc                                                                                   <  8:100418/149‑1 (MQ=255)                                                                     gcggcAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTATTCGCGGG‑TAAACCCGCGCCTACATGGGCACATATTTACCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGGCGGGTGTACATATCTGTGAGCAAAACTAt                                                                                    >  4:379408/1‑145 (MQ=38)                                                                               cATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTAGCCGCGAAGAGGCCAAGGCAGGCTGTAGATATCTGGGAGGAAAACTTTCAGATTCATCTCAg                                                                      >  6:200450/1‑149 (MQ=38)                                                                               cATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCa                                                                       <  8:83132/148‑1 (MQ=255)                                                                               cATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCa                                                                       <  4:225690/148‑1 (MQ=255)                                                                                      tCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATaaa                                                                <  3:306647/148‑1 (MQ=255)                                                                                        ccTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCa                                                                                  <  6:354193/128‑1 (MQ=255)                                                                                        ccTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCa                                                                                  >  5:354193/1‑128 (MQ=255)                                                                                        ccTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCg                                                             >  3:307118/1‑149 (MQ=255)                                                                                            ttCAG‑CTGTGCC‑‑CGCCGCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTACCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGAt                                                           <  6:181026/147‑1 (MQ=255)                                                                                                  cTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCg                                                    <  3:379408/149‑1 (MQ=255)                                                                                                             ccTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTaa                                           <  4:44601/149‑1 (MQ=255)                                                                                                               tctTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAgc                                         >  2:381956/1‑149 (MQ=255)                                                                                                                   cgcgGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAgcgc                                       <  8:359390/147‑1 (MQ=255)                                                                                                                   cgcgGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCtt                                     >  4:130243/1‑149 (MQ=255)                                                                                                                    ggggg‑TAAACCCTCTCCTACATGGCCTCATATTTCCCTGTAGGTGCAGGTTTACACGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTg                                    <  8:161645/147‑1 (MQ=255)                                                                                                                          tAAACCTGCCCCTACAGGGAAATATGTGGCCATGTAGGAGCGGGGTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAACCTATCAGATGCAGCTCAGCATAAATCGATTTTTCCGCATCTGGTAGCGCTTgatgat                               >  6:317502/1‑149 (MQ=17)                                                                                                                             aCCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATcgg                            <  3:29174/149‑1 (MQ=255)                                                                                                                               ccGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATcggcg                          >  4:327284/1‑149 (MQ=255)                                                                                                                               ccGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATcggcg                          >  6:53764/1‑149 (MQ=255)                                                                                                                                 gCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGt                        >  7:211209/1‑149 (MQ=255)                                                                                                                                     cTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCtgt                    >  3:307645/1‑149 (MQ=255)                                                                                                                                             ccACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTc             >  7:227190/1‑148 (MQ=255)                                                                                                                                                 atatTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCg        >  7:19671/1‑149 (MQ=255)                                                                                                                                                    tttCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATc         >  7:79313/1‑145 (MQ=255)                                                                                                                                                    tttCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATc         <  8:79313/145‑1 (MQ=255)                                                                                                                                                         cTGTAGGCGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCCGTCTGTGCATTTCCATCGCCTATc  >  7:153993/1‑147 (MQ=255)                                                                                                                                                         |                                                                                                                                                   GTGCTCGGCGGCGCGCACGAAGCTCTCGGTCTGGGCGGCAATGTCGAAGTAGCGGAAGGCGTTGAGTGGCGGCAGTTTCATGATATCCCTGTTCAG‑CTGTGCC‑‑GGCCTCTTCGCGGG‑TAAACCCGCTCCTACATGGCCACATATTTCCCTGTAGGGGCAGGTTTACCCGCGAAGAGGCCAAGGCAGGCTGTACATATCTGTGAGCAAAACTATCAGATTCATCTCAGCATAAATCGATTTTTCCGCATCTGTAAGCGCTTGATAATCGGCGGTCTGTGCATTTCCATCGCCTATC  >  NC_002947/812171‑812465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑