BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	856,078	G→A	6.7%	A9A (GCG→GCA)	pagL‑I →	lipid A 3‑O‑deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	856,078	0	G	A	6.7%	309.4 / 8.1	104	A9A (GCG→GCA)	pagL‑I	lipid A 3‑O‑deacylase
Reads supporting (aligned to +/- strand): ref base G (53/44); new base A (2/5); total (55/49)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 108 total aligned reads displayed.

AAAGCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTC  >  NC_002947/855931‑856226

aaaGCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCgg                                                                                                                                                     <  6:336720/149‑1 (MQ=255)

   gCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGgctg                                                                                                                                                  >  6:232245/1‑149 (MQ=255)

        gCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                              <  2:257905/148‑1 (MQ=255)

        gCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCcgctgc                                                                                                                                              <  5:276363/148‑1 (MQ=255)

          gTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                           <  4:41062/149‑3 (MQ=255)

          gTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                           <  2:205880/149‑3 (MQ=255)

             gtgAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaagc                                                                                                                                         <  1:334985/148‑5 (MQ=255)

               gAAAAAATCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGCGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaag                                                                                                                                          <  3:57199/145‑4 (MQ=255)

               gAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGttt                                                                                                                                      >  5:240510/1‑149 (MQ=255)

               gAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaag                                                                                                                                          >  4:57199/1‑142 (MQ=255)

                aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                     >  4:269644/1‑149 (MQ=255)

                 aaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTg                                                                                                                                    <  2:145159/149‑1 (MQ=255)

                  aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCG‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                   >  6:159989/1‑149 (MQ=255)

                  aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                   <  4:235368/149‑1 (MQ=255)

                  aaaaaCGTGAAAAAAGGCTGAACTATCCTTCCACTTCTGCGTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                   <  6:230250/149‑1 (MQ=255)

                   aaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTggg                                                                                                                                  <  5:212058/149‑1 (MQ=255)

                     aaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgggg                                                                                                                                 >  6:260516/1‑148 (MQ=255)

                        gTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAg                                                                                                                             <  5:260516/149‑1 (MQ=255)

                           aaaaaaGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGa                                                                                                                          >  6:35660/1‑149 (MQ=255)

                             aaaaGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATg                                                                                                                        >  4:157998/1‑149 (MQ=255)

                                 ggCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                    <  5:219827/149‑1 (MQ=255)

                                 ggCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                    <  2:188986/149‑1 (MQ=255)

                                  gCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                    >  8:222511/1‑148 (MQ=255)

                                  gCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                    >  2:195508/1‑148 (MQ=255)

                                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                     <  2:288885/129‑1 (MQ=255)

                                    tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                     >  1:288885/1‑129 (MQ=255)

                                        cTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGc                                                                                                             >  4:374609/1‑149 (MQ=255)

                                         tATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCt                                                                                                            >  3:369570/1‑149 (MQ=255)

                                           tCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCt                                                                                                            <  3:374609/147‑1 (MQ=255)

                                           tCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGc                                                                                                          <  5:232245/149‑1 (MQ=255)

                                              ttCCACTTCTGCTTTCTACCGCTTTGCCTTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGcc                                                                                                         >  1:22957/1‑148 (MQ=255)

                                                 cACTTCTGCTTTCTACCGCTTTGCC‑TGAGATTAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGCCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGTCACAGGCAGCCGAAGt                                                                                                    >  7:92327/1‑149 (MQ=255)

                                                 cACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATg                                                                                                     <  5:159989/148‑1 (MQ=255)

                                                  aCTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGtt                                                                                                   >  7:346518/1‑149 (MQ=255)

                                                   cTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGttt                                                                                                  <  1:195508/149‑1 (MQ=255)

                                                      cTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                   <  5:143437/113‑1 (MQ=255)

                                                      cTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                   >  6:143437/1‑113 (MQ=255)

                                                      cTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                                 <  7:98445/147‑1 (MQ=255)

                                                        gCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAgg                                                                                                              >  1:156684/1‑132 (MQ=255)

                                                         cTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGtttt                                                                                            <  2:307319/149‑1 (MQ=255)

                                                              tACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                       >  2:389867/1‑149 (MQ=255)

                                                              tACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                       >  2:40338/1‑149 (MQ=255)

                                                               aCCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                      >  2:231054/1‑149 (MQ=255)

                                                                 cGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgggg                                                                                    >  8:277510/1‑149 (MQ=255)

                                                                   cTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                      >  6:123747/1‑145 (MQ=255)

                                                                   cTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                      <  5:123747/145‑1 (MQ=255)

                                                                        cc‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTg                                                                             >  7:156660/1‑149 (MQ=255)

                                                                                  aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGa                                                                    >  3:20922/1‑149 (MQ=255)

                                                                                  aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGa                                                                    <  7:277510/149‑1 (MQ=255)

                                                                                    gCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGAc                                                                   >  3:324472/1‑148 (MQ=255)

                                                                                      ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                                <  5:340182/149‑1 (MQ=255)

                                                                                      ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTACTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                                >  1:56702/1‑149 (MQ=255)

                                                                                               aCAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTAcc                                                        <  1:389867/148‑1 (MQ=255)

                                                                                                cAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACa                                                                  >  6:378665/1‑137 (MQ=255)

                                                                                                cAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCgg                                                      >  1:366022/1‑149 (MQ=255)

                                                                                                 aGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGGTTGGCAGCGGGTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTTCCGATGTTTCGTTTTCGGTGGGGCAGTCTGGCGATTCGACAATGGt                                                             <  5:349830/141‑1 (MQ=255)

                                                                                                 aGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGc                                                     >  8:48955/1‑149 (MQ=255)

                                                                                                   cATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                       >  8:98381/1‑113 (MQ=255)

                                                                                                   cATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                       <  7:98381/113‑1 (MQ=255)

                                                                                                   cATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGc                                                     >  3:158852/1‑147 (MQ=255)

                                                                                                     tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                      >  5:237514/1‑112 (MQ=255)

                                                                                                     tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                      <  6:237514/112‑1 (MQ=255)

                                                                                                     tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAgg                                                 >  3:150166/1‑149 (MQ=255)

                                                                                                     tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAgg                                                 >  2:58720/1‑149 (MQ=255)

                                                                                                       agaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAggg                                                <  2:22957/148‑1 (MQ=255)

                                                                                                       agaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGc                                               >  2:161933/1‑149 (MQ=255)

                                                                                                        gaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCt                                              >  6:343449/1‑149 (MQ=255)

                                                                                                               ggATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATc                                        >  8:82267/1‑148 (MQ=255)

                                                                                                               ggATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATc                                        >  3:158226/1‑148 (MQ=255)

                                                                                                                gATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCTGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGa                                      >  6:386979/1‑149 (MQ=255)

                                                                                                                  tGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAt                                    <  8:156660/149‑1 (MQ=255)

                                                                                                                  tGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAt                                    <  5:35660/149‑1 (MQ=255)

                                                                                                                    tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                   <  1:161933/148‑1 (MQ=255)

                                                                                                                    tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                   <  3:269644/148‑1 (MQ=255)

                                                                                                                    tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                   <  4:158226/148‑1 (MQ=255)

                                                                                                                    tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                   <  4:20922/148‑1 (MQ=255)

                                                                                                                    tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                   <  7:222511/148‑1 (MQ=255)

                                                                                                                      tCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                   >  6:125530/1‑146 (MQ=255)

                                                                                                                       cTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTggg                                <  4:369570/148‑1 (MQ=255)

                                                                                                                          aTGAGGAAACTGCTCGGCTTGGCAGCGGCTACTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGa                               <  2:56702/146‑1 (MQ=255)

                                                                                                                             aGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGa                          <  8:346518/148‑1 (MQ=255)

                                                                                                                             aGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAg                         >  6:48214/1‑149 (MQ=255)

                                                                                                                              ggAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGTCCAGACTGTCGATTc                                                                      >  8:284549/1‑103 (MQ=255)

                                                                                                                              ggAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                      <  7:284549/103‑1 (MQ=255)

                                                                                                                              ggAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGt                        >  6:299073/1‑149 (MQ=255)

                                                                                                                                  aCTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggt                    <  8:92348/149‑1 (MQ=255)

                                                                                                                                    tGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGa                          <  4:316551/141‑1 (MQ=255)

                                                                                                                                    tGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGa                          >  3:316551/1‑141 (MQ=255)

                                                                                                                                      cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTTGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGtt                       <  1:53657/142‑1 (MQ=255)

                                                                                                                                      cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                  <  7:48955/147‑1 (MQ=255)

                                                                                                                                      cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCa                <  6:351836/149‑1 (MQ=255)

                                                                                                                                        cGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGa              <  1:40338/149‑1 (MQ=255)

                                                                                                                                          gCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGccgcc             >  1:138661/1‑144 (MQ=255)

                                                                                                                                          gCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc             >  4:58525/1‑148 (MQ=255)

                                                                                                                                           aaaggCAGCAGCCGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc             >  3:277623/4‑147 (MQ=255)

                                                                                                                                              ggCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCg        >  8:105468/1‑149 (MQ=255)

                                                                                                                                                cAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGtt      <  7:82267/149‑1 (MQ=255)

                                                                                                                                                 aGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTg     >  1:139096/1‑149 (MQ=255)

                                                                                                                                                    ggCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTc  <  5:299073/149‑1 (MQ=255)

                                                                                                                                                    ggCTGCTGCCGTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTc  <  6:240510/149‑1 (MQ=255)

AAAGCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTC  >  NC_002947/855931‑856226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`