BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	856,081	T→C	5.8%	A10A (GCT→GCC)	pagL‑I →	lipid A 3‑O‑deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	856,081	0	T	C	5.8%	307.5 / 6.0	103	A10A (GCT→GCC)	pagL‑I	lipid A 3‑O‑deacylase
Reads supporting (aligned to +/- strand): ref base T (52/45); new base C (2/4); total (54/49)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 108 total aligned reads displayed.

GCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGGC  >  NC_002947/855934‑856229

gCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGgctg                                                                                                                                                     >  6:232245/1‑149 (MQ=255)

     gCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCtgctgc                                                                                                                                                 <  2:257905/148‑1 (MQ=255)

     gCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCcgctgc                                                                                                                                                 <  5:276363/148‑1 (MQ=255)

       gTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                              <  2:205880/149‑3 (MQ=255)

       gTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaa                                                                                                                                              <  4:41062/149‑3 (MQ=255)

          gtgAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaagc                                                                                                                                            <  1:334985/148‑5 (MQ=255)

            gAAAAAATCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGCGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaag                                                                                                                                             <  3:57199/145‑4 (MQ=255)

            gAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGttt                                                                                                                                         >  5:240510/1‑149 (MQ=255)

            gAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCAGCCGCTGccaag                                                                                                                                             >  4:57199/1‑142 (MQ=255)

             aaaaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                        >  4:269644/1‑149 (MQ=255)

               aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCG‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                      >  6:159989/1‑149 (MQ=255)

               aaaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                      <  4:235368/149‑1 (MQ=255)

               aaaaaCGTGAAAAAAGGCTGAACTATCCTTCCACTTCTGCGTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                      <  6:230250/149‑1 (MQ=255)

                aaaaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTggg                                                                                                                                     <  5:212058/149‑1 (MQ=255)

                  aaCGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgggg                                                                                                                                    >  6:260516/1‑148 (MQ=255)

                     gTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAg                                                                                                                                <  5:260516/149‑1 (MQ=255)

                        aaaaaaGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGa                                                                                                                             >  6:35660/1‑149 (MQ=255)

                              ggCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                       <  5:219827/149‑1 (MQ=255)

                              ggCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                       <  2:188986/149‑1 (MQ=255)

                               gCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                       >  2:195508/1‑148 (MQ=255)

                               gCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCgg                                                                                                                       >  8:222511/1‑148 (MQ=255)

                                 tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                        <  2:288885/129‑1 (MQ=255)

                                 tGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGtttt                                                                                                                                        >  1:288885/1‑129 (MQ=255)

                                     cTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGc                                                                                                                >  4:374609/1‑149 (MQ=255)

                                      tATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCt                                                                                                               >  3:369570/1‑149 (MQ=255)

                                        tCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCt                                                                                                               <  3:374609/147‑1 (MQ=255)

                                        tCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGc                                                                                                             <  5:232245/149‑1 (MQ=255)

                                           ttCCACTTCTGCTTTCTACCGCTTTGCCTTGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGcc                                                                                                            >  1:22957/1‑148 (MQ=255)

                                              cACTTCTGCTTTCTACCGCTTTGCC‑TGAGATTAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGCCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGTCACAGGCAGCCGAAGt                                                                                                       >  7:92327/1‑149 (MQ=255)

                                              cACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATg                                                                                                        <  5:159989/148‑1 (MQ=255)

                                               aCTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGtt                                                                                                      >  7:346518/1‑149 (MQ=255)

                                                cTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGttt                                                                                                     <  1:195508/149‑1 (MQ=255)

                                                   cTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                      <  5:143437/113‑1 (MQ=255)

                                                   cTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTgg                                                                                                                                      >  6:143437/1‑113 (MQ=255)

                                                   cTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTc                                                                                                    <  7:98445/147‑1 (MQ=255)

                                                     gCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAgg                                                                                                                 >  1:156684/1‑132 (MQ=255)

                                                      cTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGtttt                                                                                               <  2:307319/149‑1 (MQ=255)

                                                           tACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                          >  2:40338/1‑149 (MQ=255)

                                                           tACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                          >  2:389867/1‑149 (MQ=255)

                                                            aCCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                         >  2:231054/1‑149 (MQ=255)

                                                              cGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgggg                                                                                       >  8:277510/1‑149 (MQ=255)

                                                                cTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                         >  6:123747/1‑145 (MQ=255)

                                                                cTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                         <  5:123747/145‑1 (MQ=255)

                                                                     cc‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTg                                                                                >  7:156660/1‑149 (MQ=255)

                                                                               aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGa                                                                       <  7:277510/149‑1 (MQ=255)

                                                                               aGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGa                                                                       >  3:20922/1‑149 (MQ=255)

                                                                                 gCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGAc                                                                      >  3:324472/1‑148 (MQ=255)

                                                                                   ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                                   <  5:340182/149‑1 (MQ=255)

                                                                                   ggACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTACTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAAt                                                                   >  1:56702/1‑149 (MQ=255)

                                                                                            aCAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTAcc                                                           <  1:389867/148‑1 (MQ=255)

                                                                                             cAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACa                                                                     >  6:378665/1‑137 (MQ=255)

                                                                                             cAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCgg                                                         >  1:366022/1‑149 (MQ=255)

                                                                                              aGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGGTTGGCAGCGGGTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTTCCGATGTTTCGTTTTCGGTGGGGCAGTCTGGCGATTCGACAATGGt                                                                <  5:349830/141‑1 (MQ=255)

                                                                                              aGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGc                                                        >  8:48955/1‑149 (MQ=255)

                                                                                              aGCATCAGAAAGAAGGATGATTCTTAAGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGGCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGAAGACTGGAGAATCGACAATGGt                                                                >  6:349830/1‑141 (MQ=255)

                                                                                                cATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                          >  8:98381/1‑113 (MQ=255)

                                                                                                cATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTg                                                                                          <  7:98381/113‑1 (MQ=255)

                                                                                                cATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGc                                                        >  3:158852/1‑147 (MQ=255)

                                                                                                  tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                         >  5:237514/1‑112 (MQ=255)

                                                                                                  tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTgg                                                                                         <  6:237514/112‑1 (MQ=255)

                                                                                                  tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAgg                                                    >  2:58720/1‑149 (MQ=255)

                                                                                                  tCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAgg                                                    >  3:150166/1‑149 (MQ=255)

                                                                                                    agaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAggg                                                   <  2:22957/148‑1 (MQ=255)

                                                                                                    agaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGc                                                  >  2:161933/1‑149 (MQ=255)

                                                                                                     gaaagaaGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCt                                                 >  6:343449/1‑149 (MQ=255)

                                                                                                            ggATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATc                                           >  3:158226/1‑148 (MQ=255)

                                                                                                            ggATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATc                                           >  8:82267/1‑148 (MQ=255)

                                                                                                             gATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCTGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGa                                         >  6:386979/1‑149 (MQ=255)

                                                                                                               tGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAt                                       <  8:156660/149‑1 (MQ=255)

                                                                                                               tGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAt                                       <  5:35660/149‑1 (MQ=255)

                                                                                                                 tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                      <  1:161933/148‑1 (MQ=255)

                                                                                                                 tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                      <  7:222511/148‑1 (MQ=255)

                                                                                                                 tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                      <  3:269644/148‑1 (MQ=255)

                                                                                                                 tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                      <  4:158226/148‑1 (MQ=255)

                                                                                                                 tttCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                      <  4:20922/148‑1 (MQ=255)

                                                                                                                   tCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAAtt                                      >  6:125530/1‑146 (MQ=255)

                                                                                                                    cTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTggg                                   <  4:369570/148‑1 (MQ=255)

                                                                                                                         gAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGAcgc                               >  4:116266/1‑147 (MQ=255)

                                                                                                                          aGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGa                             <  8:346518/148‑1 (MQ=255)

                                                                                                                          aGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAg                            >  6:48214/1‑149 (MQ=255)

                                                                                                                           ggAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGTCCAGACTGTCGATTc                                                                         >  8:284549/1‑103 (MQ=255)

                                                                                                                           ggAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTc                                                                         <  7:284549/103‑1 (MQ=255)

                                                                                                                           ggAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGt                           >  6:299073/1‑149 (MQ=255)

                                                                                                                               aCTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggt                       <  8:92348/149‑1 (MQ=255)

                                                                                                                                 tGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGa                             <  4:316551/141‑1 (MQ=255)

                                                                                                                                 tGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGa                             >  3:316551/1‑141 (MQ=255)

                                                                                                                                   cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTTGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGtt                          >  2:53657/1‑142 (MQ=255)

                                                                                                                                   cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTTGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGtt                          <  1:53657/142‑1 (MQ=255)

                                                                                                                                   cTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTtggtgg                     <  7:48955/147‑1 (MQ=255)

                                                                                                                                     cGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGa                 <  1:40338/149‑1 (MQ=255)

                                                                                                                                       gCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGccgcc                >  1:138661/1‑144 (MQ=255)

                                                                                                                                       gCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc                >  4:58525/1‑148 (MQ=255)

                                                                                                                                        aaaggCAGCAGCCGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGAc                >  3:277623/4‑147 (MQ=255)

                                                                                                                                           ggCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCg           >  8:105468/1‑149 (MQ=255)

                                                                                                                                             cAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGtt         <  7:82267/149‑1 (MQ=255)

                                                                                                                                              aGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTg        >  1:139096/1‑149 (MQ=255)

                                                                                                                                                 ggCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTc     <  5:299073/149‑1 (MQ=255)

                                                                                                                                                 ggCTGCTGCCGTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTc     <  6:240510/149‑1 (MQ=255)

                                                                                                                                                   ctgctgCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCgg   <  4:158852/149‑1 (MQ=255)

                                                                                                                                                    tgctgcCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGGc  >  7:362166/1‑149 (MQ=255)

GCTTTGCGTTGTGAAAAAAACGTGAAAAAAGGCTGAACTATCGTTCCACTTCTGCTTTCTACCGCTTTGCC‑TGAGATGAGGCGGACAAATAACAGCATCAGAAAGAAGGATGTTTCTTATGAGGAAACTGCTCGGCTTGGCAGCGGCTGCTGCCTTTGTTTTGGGGCAAGCGATGTCGGCACAGGCTGCCGATGTTTCGTTTTCGGTGGGGCAGACTGGCGATTCGACAATGGTCTACCGGCTAGGGCTGCAATCGAATTGGGACGCGAGTTGGTGGCAGACCAGCGTTGGTCGGC  >  NC_002947/855934‑856229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`