Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,439 C→T 6.1% A4395T (GCC→ACC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,4390CT 6.1% 251.7 / 3.9 82A4395T (GCC→ACC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (42/35);  new base T (3/2);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGC  >  NC_002947/932296‑932580
                                                                                                                                               |                                                                                                                                             
cGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTg                                                                                                                                          >  8:95615/1‑149 (MQ=255)
  gCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTa                                                                                                                                        <  1:96067/149‑1 (MQ=255)
    gTGGCCGGTGCCAGCGCGCCCGGCGCCGGCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGc                                                                                                                                      >  4:299219/1‑149 (MQ=255)
         cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                 >  1:241140/1‑149 (MQ=255)
          ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                >  3:86389/1‑149 (MQ=255)
               cAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCa                                                                                                                           >  6:192493/1‑149 (MQ=255)
                aGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCAc                                                                                                                          >  4:131576/1‑149 (MQ=255)
                aGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCAc                                                                                                                          >  7:117539/1‑149 (MQ=255)
                 gcgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACg                                                                                                                         >  6:95884/1‑149 (MQ=255)
                    cgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGAGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTa                                                                                                                      <  4:47805/149‑1 (MQ=255)
                     gcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTAc                                                                                                                     >  3:279821/1‑149 (MQ=255)
                      cccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                >  3:55082/1‑137 (MQ=255)
                      cccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                <  4:55082/137‑1 (MQ=255)
                               gTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCCGCACGGTACCGTGCGGTGc                                                                                                           >  8:129476/1‑149 (MQ=255)
                                  aCGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTc                                                                                                         <  3:112423/148‑1 (MQ=255)
                                          cgcAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTc                                                                                                 <  2:359649/148‑1 (MQ=255)
                                             aGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACgg                                                                                             <  5:168614/149‑1 (MQ=255)
                                                 gagaCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgc                                                                                         <  7:95615/149‑1 (MQ=255)
                                                  agaCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcg                                                                                        <  8:117539/149‑1 (MQ=255)
                                                   gaCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcgc                                                                                       <  5:95884/149‑1 (MQ=255)
                                                    aCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGcc                                                                                      >  7:94536/1‑149 (MQ=255)
                                                            tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGc                                                                              >  3:143252/1‑149 (MQ=255)
                                                                 cATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCg                                                                         >  2:240399/1‑149 (MQ=255)
                                                                  aTCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTc                                                                                <  8:23195/141‑1 (MQ=255)
                                                                  aTCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTc                                                                                >  7:23195/1‑141 (MQ=255)
                                                                  aTCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGc                                                                        >  8:22233/1‑149 (MQ=255)
                                                                      gCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                 >  3:237567/1‑104 (MQ=255)
                                                                      gCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                 <  4:237567/104‑1 (MQ=255)
                                                                          ggACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAgg                                                                >  5:215469/1‑149 (MQ=255)
                                                                          ggACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACAGTCCGGTGCTCGTACCGTAACGGTTGCGCCTGCTTCGCCATAGCCTGTCAgg                                                                >  3:144702/1‑149 (MQ=255)
                                                                             ccACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTg                                                             >  8:255248/1‑149 (MQ=255)
                                                                                   tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACg                                                                                              >  6:56748/1‑110 (MQ=255)
                                                                                   tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACg                                                                                              <  5:56748/110‑1 (MQ=255)
                                                                                   tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCa                                                       >  7:208737/1‑149 (MQ=255)
                                                                                   tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCa                                                       >  4:98803/1‑149 (MQ=255)
                                                                                     aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                     <  8:170783/149‑1 (MQ=255)
                                                                                     aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                     <  6:215469/149‑1 (MQ=255)
                                                                                     aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                     >  7:227421/1‑149 (MQ=255)
                                                                                     aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                     <  6:220486/149‑1 (MQ=255)
                                                                                     aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                     <  2:241140/149‑1 (MQ=255)
                                                                                       gCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCGGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTgg                                                   >  4:201301/1‑149 (MQ=255)
                                                                                       gCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcgc                                                                                       <  6:140416/113‑1 (MQ=255)
                                                                                       gCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcgc                                                                                       >  5:140416/1‑113 (MQ=255)
                                                                                       gCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTgg                                                   >  1:312053/1‑149 (MQ=255)
                                                                                         tttCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCg                                                 >  2:190933/1‑149 (MQ=255)
                                                                                          ttCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCg                                                 >  2:49459/1‑148 (MQ=255)
                                                                                           tCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCgg                                                <  4:86389/148‑1 (MQ=255)
                                                                                            cACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGga                                               <  3:131576/148‑1 (MQ=255)
                                                                                            cACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGga                                               <  7:255248/148‑1 (MQ=255)
                                                                                            cACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGga                                               <  4:143252/148‑1 (MQ=255)
                                                                                             aCCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                             >  8:20856/1‑149 (MQ=255)
                                                                                                 gTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                             >  7:139960/1‑145 (MQ=255)
                                                                                                 gTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                             <  8:139960/145‑1 (MQ=255)
                                                                                                       gCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTg                                                    <  3:116538/132‑1 (MQ=255)
                                                                                                       gCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTg                                                    >  4:116538/1‑132 (MQ=255)
                                                                                                        cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                  <  4:279821/149‑1 (MQ=255)
                                                                                                           ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                <  7:22233/148‑1 (MQ=255)
                                                                                                           ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                <  5:147081/148‑1 (MQ=255)
                                                                                                            cGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTGCCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCa                              >  3:143232/1‑149 (MQ=255)
                                                                                                              gTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTAGTACCGTCACCGTTGCGCCTGCCTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcg                            >  4:43007/1‑149 (MQ=255)
                                                                                                               tGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGctcacg                           >  5:373207/1‑144 (MQ=255)
                                                                                                                gACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggc                          >  6:124019/1‑149 (MQ=255)
                                                                                                                  cAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcgg                        <  5:192493/149‑1 (MQ=255)
                                                                                                                     ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccg                     >  7:330695/1‑149 (MQ=255)
                                                                                                                     ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccg                     >  4:276689/1‑149 (MQ=255)
                                                                                                                           gCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGgcggcg               >  3:19712/1‑149 (MQ=255)
                                                                                                                            ctacaGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgt              >  6:136341/5‑149 (MQ=255)
                                                                                                                            cTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgt              <  3:276689/149‑1 (MQ=255)
                                                                                                                               caGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAg                                                                 <  4:81697/94‑1 (MQ=255)
                                                                                                                               caGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAg                                                                 >  3:81697/2‑95 (MQ=255)
                                                                                                                               caGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtggt           >  3:162353/2‑149 (MQ=255)
                                                                                                                               aaGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGc                                         <  3:141792/119‑1 (MQ=255)
                                                                                                                               aaGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGc                                         >  4:141792/1‑119 (MQ=255)
                                                                                                                               aaGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtggt           >  7:275918/1‑149 (MQ=255)
                                                                                                                               aaGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtg             <  5:124019/147‑1 (MQ=255)
                                                                                                                               aaGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGCCGCCGGCCGCCgtgg            <  1:240399/148‑1 (MQ=255)
                                                                                                                                 gTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGgtgt         <  6:206900/149‑1 (MQ=255)
                                                                                                                                   gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACa                                           <  4:53282/113‑1 (MQ=255)
                                                                                                                                   gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACa                                           >  3:53282/1‑113 (MQ=255)
                                                                                                                                    gccgccgACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCg      <  4:162353/148‑1 (MQ=255)
                                                                                                                                       gTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTg   <  2:313684/149‑1 (MQ=255)
                                                                                                                                        tCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGc  <  8:227421/149‑1 (MQ=255)
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CGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGC  >  NC_002947/932296‑932580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑