Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	943,988	T→A	5.9%	D545V (GAT→GTT)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	943,988	0	T	A	5.9%	234.2 / 3.5	83	D545V (GAT→GTT)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (37/41);  new base A (3/2);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGTTCGGTCGGTGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTA  >  NC_002947/943841‑944135                                                                                                                                                     |                                                                                                                                                     gCCAGTTCGGTCGGTGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATc                                                                                                                                                     <  6:241171/149‑1 (MQ=32)    aGTTCGGTCGGTGCGGCAGGGGCGGTGATGACGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGc                                                                                                                                                  >  1:36310/1‑149 (MQ=17)     gTTCGGTCGGTGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCg                                                                                                                                                 >  1:357592/1‑149 (MQ=32)        cggtcggtGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGtt                                                                                                                                               <  6:65291/148‑1 (MQ=32)        cggtcggtGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTg                                                                                                                                              >  5:78306/1‑149 (MQ=32)          gtcggtGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGc                                                                                                                                            <  4:118228/149‑1 (MQ=32)             ggtGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAAt                                                                                                                                         >  2:198527/1‑149 (MQ=32)                     aGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGc                                                                                                                                 <  6:217090/149‑1 (MQ=32)                       gggCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGcc                                                                                                                                >  3:350332/1‑148 (MQ=32)                       gggCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCa                                                                                                                               <  6:78306/149‑1 (MQ=32)                           ggTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCa                                                                                                                           <  2:106452/149‑1 (MQ=32)                             tGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGc                                                                                                                         >  1:304161/1‑149 (MQ=33)                              gATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGc                                                                                                                         <  7:1228/148‑1 (MQ=33)                               aTGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCGTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑Gt                                                                                                                      >  5:356396/1‑149 (MQ=21)                                tggcGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCCCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTg                                                                                                                     <  6:244558/146‑1 (MQ=21)                                  tCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAATGTCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGATGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGc                                                                                                                    >  1:120911/1‑149 (MQ=255)                                    gggCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGt                                                                                                                 >  7:192554/1‑149 (MQ=34)                                     ggCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGtt                                                                                                                <  7:251494/149‑1 (MQ=34)                                        gcgGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGc                                                                                                             <  6:28941/149‑1 (MQ=34)                                          ggTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGt                                                                                                           >  5:313419/1‑149 (MQ=35)                                           gTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGt                                                                                                           >  5:190703/1‑148 (MQ=35)                                              aTCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCAt                                                                                                        <  6:190703/148‑1 (MQ=37)                                              aTCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATg                                                                                                       >  4:330852/1‑149 (MQ=37)                                                cTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCa                                                                                                     >  2:283682/1‑149 (MQ=37)                                                  gtgCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCAcc                                                                                                   >  8:325085/1‑149 (MQ=38)                                                  gtgCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCAcc                                                                                                   >  1:362185/1‑149 (MQ=38)                                                   tgCGGGCAGCGAGGAAACGCCGCCGCCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGc                                                                                                      <  5:342780/145‑1 (MQ=37)                                                   tgCGGGCACCGAGGAAACGCAGCCGTCATCGATGGCAACCACGTCCAGCAGG‑TCGCCGTTGGCTTGTGCGGGAATCACATCGATGCTGAAGCTGCCATCGGCGTTGTCAAGGGCACTGCCAATCAGC‑GCGCCGTTGGCGTCATGCACCt                                                                                                  <  6:88556/149‑1 (MQ=11)                                                            cGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGc                                                                                         >  8:140521/1‑149 (MQ=39)                                                             gAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAAt                                                                                                                             <  8:279340/113‑1 (MQ=32)                                                                 aaaCGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCa                                                                                    <  2:226912/149‑1 (MQ=39)                                                                   aCGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGc                                                                                  <  8:192554/149‑1 (MQ=39)                                                                   aCGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGc                                                                                  >  1:152993/1‑149 (MQ=39)                                                                      ccgccgTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCgg                                                                               <  7:153476/149‑1 (MQ=39)                                                                         ccgTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGgcgc                                                                            <  4:350332/149‑1 (MQ=39)                                                                          cgTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATAGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGcgcg                                                                           >  7:51388/1‑149 (MQ=255)                                                                               tCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTAAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTAcc                                                                      >  7:362428/1‑149 (MQ=255)                                                                                cGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCgg                                                                          <  4:94135/144‑1 (MQ=39)                                                                                cGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCgg                                                                          >  3:94135/1‑144 (MQ=39)                                                                                   tGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTg                                                                  <  2:36310/149‑1 (MQ=39)                                                                                       aaCCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAggg                                                              >  6:18650/1‑149 (MQ=38)                                                                                        aCCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGggt                                                             <  4:183227/149‑1 (MQ=37)                                                                                             gTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGc                                                                                  <  2:98399/123‑1 (MQ=38)                                                                                             gTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGc                                                                                  >  1:98399/1‑123 (MQ=38)                                                                                                 agcagT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTcc                                                                                                <  4:42698/105‑1 (MQ=40)                                                                                                 agcagT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTcc                                                                                                >  3:42698/1‑105 (MQ=40)                                                                                                 agcagT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATc                                                    <  6:327801/149‑1 (MQ=37)                                                                                                     gT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGc                                                 >  7:319342/1‑148 (MQ=37)                                                                                                      t‑tCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCa                                                <  2:357592/148‑1 (MQ=37)                                                                                                           ccGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGAt                                           <  2:304161/149‑1 (MQ=37)                                                                                                           ccGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGAt                                           <  1:198527/149‑1 (MQ=37)                                                                                                             gTTGGCTTGTGCGGGATGCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATgg                                         <  3:330852/149‑1 (MQ=37)                                                                                                                ggCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGcc                                       <  2:120911/148‑1 (MQ=37)                                                                                                                      tgCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGc                                <  2:320657/149‑1 (MQ=37)                                                                                                                         gggATTCAGTTCGATGCTAAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCg                             <  8:362428/149‑1 (MQ=37)                                                                                                                           gATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCggg                           >  4:283787/1‑149 (MQ=37)                                                                                                                           gATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGCCTGACAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCggg                           >  6:135775/1‑149 (MQ=255)                                                                                                                            aTTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGc                          >  2:205815/1‑149 (MQ=37)                                                                                                                                aGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTc                      >  5:200808/1‑149 (MQ=37)                                                                                                                                  ttCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCgg                    >  3:197819/1‑149 (MQ=37)                                                                                                                                   tCGTTGCTGAAGCTGCCATGGGCGTTGGCAATGGCACTGCCAATGAGCGGTGCGGTTGG‑GTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGGGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGGCgg                    <  7:27788/148‑1 (MQ=11)                                                                                                                                    cGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGgc                  >  7:24463/1‑149 (MQ=37)                                                                                                                                     gATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGgcg                 >  1:71354/1‑149 (MQ=37)                                                                                                                                          tGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCggtg            >  5:266912/1‑149 (MQ=37)                                                                                                                                            ccattGCCAACGCCGATGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCggtggt          >  1:347701/5‑149 (MQ=255)                                                                                                                                            ccattGCCAACGCCGATGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACATCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCggtggt          >  8:88162/5‑149 (MQ=11)                                                                                                                                            aaGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCCCACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCggtggt          <  6:313419/149‑1 (MQ=37)                                                                                                                                             cattGCCAACGCCGATGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGGGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTc         >  8:79900/4‑149 (MQ=11)                                                                                                                                              gCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCa                                                   >  1:36995/1‑106 (MQ=40)                                                                                                                                              gCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCa                                                   <  2:36995/106‑1 (MQ=40)                                                                                                                                              gCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCgg                    >  7:299020/1‑137 (MQ=37)                                                                                                                                              gCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCgg                    <  8:299020/137‑1 (MQ=37)                                                                                                                                              gCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCggtggt          >  2:310862/1‑147 (MQ=37)                                                                                                                                                tGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGt      >  3:127077/1‑149 (MQ=37)                                                                                                                                                 gCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATTCACCTCCACACTGCTACCAGCCGGCGCGGTACCGTTGATGGTTGTGCCATCAGCATCGATTGCCTGATTGGTCGTGATGTCGGGCGCGGTGGTCAGTg     <  6:356396/149‑1 (MQ=11)                                                                                                                                                 gCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTg     >  4:170972/1‑149 (MQ=37)                                                                                                                                                 gCCAACGCCGATGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGt      <  7:88162/148‑1 (MQ=255)                                                                                                                                                 gCCAACGCCGATGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGt      <  2:347701/148‑1 (MQ=255)                                                                                                                                                  ccctCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGGCATGCACCGCCCCACGGCTGCCAGCCTGCGCGCTACCGGGGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTg     <  2:301801/145‑1 (MQ=11)                                                                                                                                                  ccATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTg     <  5:18650/148‑1 (MQ=37)                                                                                                                                                   cATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATTCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGt   <  4:293812/149‑1 (MQ=37)                                                                                                                                                   cATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGt   <  8:24463/149‑1 (MQ=37)                                                                                                                                                   cATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGt   <  8:319342/149‑1 (MQ=37)                                                                                                                                                    aTCGGCGTTGGCAATGGCACTGCCAATCAGA‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTa  <  7:325085/149‑1 (MQ=37)                                                                                                                                                     tCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTa  >  3:75169/1‑148 (MQ=37)                                                                                                                                                     |                                                                                                                                                     GCCAGTTCGGTCGGTGCGGCAGGGGCGGTGATGTCGGGCGCGGTGATCTGTGCGGGCAGCGAGGAAACGCCGCCGTCATCGATGGCAACCACGTCCAGCAGT‑TCGCCGTTGGCTTGTGCGGGATTCAGTTCGATGCTGAAGCTGCCATCGGCGTTGGCAATGGCACTGCCAATCAGC‑GTGCCGTTGGCGTCATGCACCTCCACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTA  >  NC_002947/943841‑944135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑