| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,152 | T→A | 26.2% | intergenic (+106/‑207) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,152 | 0 | T | A | 26.2% | 52.8 / 17.0 | 34 | intergenic (+106/‑207) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (13/12); new base A (5/4); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAT > NC_002947/955004‑955293 | cAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGTGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCAGGTAAACCCGCTCCTACAAGGt < 8:165406/149‑1 (MQ=255) gCCAGACCGCTGCGGATTTTCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTc < 4:41731/149‑1 (MQ=255) gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGt < 5:351027/149‑1 (MQ=255) gACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGt < 8:143280/149‑1 (MQ=255) cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGc < 1:4243/149‑1 (MQ=37) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTTCTGTGCTGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCgg < 1:243103/149‑1 (MQ=25) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 2:329028/149‑1 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 6:318161/149‑1 (MQ=255) cGGATGTGATGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 6:94038/149‑1 (MQ=255) aTGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGTGGTTTTTTTTTGGGGGTATTTTTATTTTTCTGTGCTGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGc < 2:31190/149‑1 (MQ=18) tgatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg > 4:240614/1‑141 (MQ=37) tgatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg < 3:240614/141‑1 (MQ=37) ctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt > 5:117974/1‑105 (MQ=255) ctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt > 5:123315/1‑105 (MQ=255) ctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt < 6:117974/105‑1 (MQ=255) ctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt < 6:123315/105‑1 (MQ=255) aaCCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCCAGATTCGCGGGTAAACCCGGTCGTACAAGGTTCCTGTTGGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAATTAAAAATACCTGTtttt > 1:300125/1‑148 (MQ=37) c‑tctTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATc > 1:104434/1‑149 (MQ=255) gcgGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATc > 6:88777/1‑130 (MQ=255) gcgGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATc < 5:88777/130‑1 (MQ=255) gggTAAACCCGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCg > 8:280095/1‑149 (MQ=37) cccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt > 7:248848/1‑149 (MQ=255) ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg < 4:33926/142‑1 (MQ=255) ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg > 3:33926/1‑142 (MQ=255) ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc > 3:208477/1‑149 (MQ=255) cGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 4:41754/1‑149 (MQ=37) gCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt > 7:331660/1‑149 (MQ=38) gCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtctt > 7:99959/1‑149 (MQ=38) cTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAGAGGCAGGCTGTGTCGGCAGTATCTTCTGa > 8:271839/1‑149 (MQ=39) cTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa > 7:98211/1‑149 (MQ=255) atACAATTTTCCTTTAGGAGCGGTTTTACCCGCTAAGAGGCCAGCACAGAAAAATAAAAATAACTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttct < 7:53398/146‑1 (MQ=255) tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 6:37493/1‑149 (MQ=255) aCAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCttt > 7:36496/1‑116 (MQ=255) aCAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCttt < 8:36496/116‑1 (MQ=255) aCAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 3:126000/1‑148 (MQ=255) | CAGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTAT‑‑‑‑‑TTTTCCTGTGCCGGCC‑TCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAT > NC_002947/955004‑955293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |