| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 985,937 | G→C | 8.7% | L233F (TTG→TTC) | pilF → | type IV pili biogenesis protein PilF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 985,937 | 0 | G | C | 8.7% | 185.9 / 4.6 | 65 | L233F (TTG→TTC) | pilF | type IV pili biogenesis protein PilF |
| Reads supporting (aligned to +/- strand): ref base G (28/30); new base C (3/3); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGAAACGG > NC_002947/985790‑986083 | tGAGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑Tgg > 3:237401/1‑149 (MQ=255)tGAGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑Tgg < 8:297183/149‑1 (MQ=255) aGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑Tgg < 4:274564/147‑1 (MQ=255) aGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑Tgg < 8:189455/147‑1 (MQ=255) aGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑Tgg < 1:66032/147‑1 (MQ=255) aGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGc < 8:216625/149‑1 (MQ=255) gTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAg < 2:213560/148‑1 (MQ=255) tCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑CA‑‑‑AT‑‑TCGGCCAgt < 3:67665/148‑2 (MQ=255) ccTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑CA‑‑‑AT‑‑TCGGCCAgtgt < 7:106375/149‑4 (MQ=255) cTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAAt > 2:126379/1‑149 (MQ=255) cTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑CA‑‑‑AT‑‑TCGGCCAgtgt > 8:100820/1‑145 (MQ=255) cTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑CA‑‑‑AT‑‑TCGGCCAgtgt > 8:106375/1‑145 (MQ=255) cTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑CA‑‑‑AT‑‑TCGGCCAgtgt < 7:100820/148‑4 (MQ=255) tACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAAt > 6:329184/1‑148 (MQ=255) cGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGGCTGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAAtt < 1:270705/148‑1 (MQ=255) cGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTAc < 2:234854/149‑1 (MQ=255) gAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTAc > 1:129200/1‑148 (MQ=255) aaaCATTCATTATGTGCCGGCCCTGGACTACTACGTTCGTTTCATCCATTTTAGCGAACACGACGCCCTTAGCCTGCTGCTGGGCAGCCGCCTTGCTATGG‑TGTTCGACGATCATGTCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTAc < 7:242947/146‑1 (MQ=255) aCAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGa > 7:281399/1‑149 (MQ=255) ccGGGACTACTAGGATCGTTTCAGACAGTTGAGCGACCACGACGCCAGTAGACTGCTGCTGGGCAGCCGCCTTGCCAAGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGtcatcac > 8:214509/1‑142 (MQ=255) ctactaCGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCCTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATc < 5:329184/149‑1 (MQ=255) ctacGATCGTTTCATCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTTGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCa < 7:325193/147‑1 (MQ=255) ctacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAg < 4:237401/148‑1 (MQ=255) tacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGc > 1:384397/1‑148 (MQ=255) tacGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGc > 1:370004/1‑148 (MQ=255) gATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAAt > 2:313634/1‑148 (MQ=255) aTCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAGCGACt < 6:220621/118‑1 (MQ=255) tttCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGt > 3:50203/1‑149 (MQ=255) gCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAg > 5:353725/1‑149 (MQ=255) gCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAg < 2:296966/149‑1 (MQ=255) gCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAg > 8:105288/1‑149 (MQ=255) gTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAAc > 4:87489/1‑149 (MQ=255) aGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGAt < 5:155452/148‑1 (MQ=255) aCCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAAcg > 6:271559/1‑149 (MQ=255) gacgCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCcgcg > 8:92006/1‑149 (MQ=255) acccccGAAGCCTGCTCCTGGGGAGCCGCCTAGCCACGC‑TGTTCGACGAGCAGGGCCCACTGGCC‑‑‑GA‑‑‑GA‑‑TGGGCCAGGAAGTACAACGACTTTAACCCGGTACCCCGGAATATCAGCAATACCTGTCGGGGCAACGCTGCACGCCgagc > 6:351772/4‑149 (MQ=255) ccGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATcc < 2:384397/149‑1 (MQ=255) gCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGaa < 2:351118/148‑1 (MQ=255) ccTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGAGGAACGCCGCGCATCCCGAagt > 4:47843/1‑149 (MQ=255) ctgctgctgGGCAGCCGCCTTGCCAGGG‑TTTTCCACGCGCTGGTCACACTGGCCTCACATCCATCCTACACCCGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATc < 1:126379/149‑1 (MQ=255) ctgctgctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCaa > 2:3454/1‑108 (MQ=255) ctgctgctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCaa < 1:3454/108‑1 (MQ=255) ctgctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtag < 1:313634/147‑1 (MQ=255) tgctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtagcag > 3:333867/1‑149 (MQ=255) tgctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTCGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGGATCCCGAagtag > 4:223119/1‑146 (MQ=255) ctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtagcag < 3:87489/147‑1 (MQ=255) ctgGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtagcag > 3:155864/1‑147 (MQ=255) ggCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagtagcagtagc < 8:281399/148‑1 (MQ=255) cAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGcc < 7:92006/149‑1 (MQ=255) ccgccTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagt > 2:209820/1‑134 (MQ=255) ccgccTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAagt < 1:209820/134‑1 (MQ=255) gccTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc < 7:105288/149‑1 (MQ=255) cTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGc > 6:378486/1‑146 (MQ=255) cAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAAccc > 1:98620/1‑149 (MQ=255) gg‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCgg > 2:311769/1‑149 (MQ=255) ttCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAg > 2:354904/1‑149 (MQ=255) cgacgaGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGc < 2:370004/148‑1 (MQ=255) cgaGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCGAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCtttt < 8:97522/149‑1 (MQ=255) cgaGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCGGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATGCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCtttt < 2:129200/149‑1 (MQ=255) aGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTgc < 4:155864/149‑1 (MQ=255) aGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc > 7:294909/1‑125 (MQ=255) aGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc > 7:289702/1‑125 (MQ=255) aGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc < 8:289702/125‑1 (MQ=255) aGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGcc < 8:294909/125‑1 (MQ=255) ggCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTg < 6:353725/149‑1 (MQ=255) attgcTGGCC‑‑‑CA‑‑‑AT‑‑TCGGCCAGCAATTACAACGAATTTATCCCGGTAAGCCGGAATATCAGCAATACCTGTCGGATCAACGATGAACGCCGAGCATACCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGAGCCTGGc > 2:147365/5‑149 (MQ=255) acacTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGcc > 8:239272/1‑149 (MQ=255) gCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTga < 5:378486/148‑1 (MQ=255) a‑‑‑aT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGAAAc < 4:50203/149‑1 (MQ=255) t‑‑tGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGAAACgg < 4:333867/149‑1 (MQ=255) | TGAGTTGTCCTACGAAAACAGGCATTATGTGCCGGCCCGGGACTACTACGATCGTTTCAGCCAGTTGAGCGACCACGACGCCCGTAGCCTGCTGCTGGGCAGCCGCCTTGCCAGGG‑TGTTCGACGAGCAGGGCACACTGGCC‑‑‑GA‑‑‑AT‑‑TGGGCCAGCAATTACAACGACTTTATCCCGGTACGCCGGAATATCAGCAATACCTGTCGGAGCAACGATGAACGCCGCGCATCCCGAAGTAGCAGTAGCGCCTGGCCAGAACCCCGGTGAGCTTTTGCGCCTGGCCCGTGAGAAACGG > NC_002947/985790‑986083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |