Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,006,967 A→T 7.5% T633S (ACC→TCC)  PP_0867 → FecA‑like outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,006,9670AT 7.5% 146.6 / 2.9 53T633S (ACC→TCC) PP_0867FecA‑like outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

CGGCCAATGGCCTGGAGCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAGGGGATATTCCAGGGTTC  >  NC_002947/1006824‑1007113
                                                                                                                                               |                                                                                                                                                  
cGGCCAATGGCCTGGAGCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAAc                                                                                                                                               <  5:108552/149‑1 (MQ=255)
         gCCTGGAGCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGc                                                                                                                                       <  1:230385/148‑1 (MQ=255)
          ccTTGAGCCGGAAAAAGCCACGACCTACCAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCACTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGa                                                                                                                                     <  8:290126/149‑1 (MQ=255)
           cTGGAGCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGAc                                                                                                                                    >  1:356110/1‑149 (MQ=255)
               aGCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAg                                                                                                                                <  3:107902/149‑1 (MQ=255)
                gCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGc                                                                                                                               <  3:188595/149‑1 (MQ=255)
                           ccAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATc                                                                                                                    >  6:201950/1‑149 (MQ=255)
                           ccAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATc                                                                                                                    <  1:361345/149‑1 (MQ=255)
                              ataCCTACGAAATCGGTAGCGGTTATGACATTGGCGGCTTTGGCGTTGATCTGACGGCGTTCTTCATGGACTTCGATGATGAGCTGGAGTACATCAGCAACGACGTTGGCTGGACCAACCTCGGTGCGACCAAGCACGATGGTATCGAg                                                                                                                 <  4:168965/147‑1 (MQ=255)
                                  cTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCtt                                                                                                             >  1:331514/1‑149 (MQ=255)
                                   tACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTc                                                                                                            >  8:83452/1‑149 (MQ=255)
                                   tACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTc                                                                                                            >  3:248666/1‑149 (MQ=255)
                                   tACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTc                                                                                                            >  3:199164/1‑149 (MQ=255)
                                     cGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCgg                                                                                                          >  7:114562/1‑149 (MQ=255)
                                     cGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCgg                                                                                                          >  4:279180/1‑149 (MQ=255)
                                      gAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGt                                                                                                         <  1:335502/149‑1 (MQ=255)
                                        actCGGTACCGGTTATGACAATGGCTGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGTTGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACGAACCTCGGTGCGACCAAGGACCAGGGTATCGAGGCTTCGGTGc                                                                                                       <  4:342599/147‑1 (MQ=255)
                                        aaTCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGc                                                                                                       <  5:201950/149‑1 (MQ=255)
                                            ggTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGtt                                                                                                    <  4:248666/148‑1 (MQ=255)
                                            ggTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTa                                                                                                   >  8:357587/1‑149 (MQ=255)
                                                 ccGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGAcc                                                                                              >  6:65946/1‑149 (MQ=255)
                                                  cGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCt                                                                                             >  2:179974/1‑149 (MQ=255)
                                                          cAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGc                                                                                     >  5:297596/1‑149 (MQ=255)
                                                                 ggcTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGAccc                                                                              <  3:279180/149‑1 (MQ=255)
                                                                        ccGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCt                                                                        <  6:297596/148‑1 (MQ=255)
                                                                         cGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGc                                                                                     <  4:190197/134‑1 (MQ=255)
                                                                         cGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCgcgc                                                                          >  6:389706/1‑145 (MQ=255)
                                                                         cGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCgcgc                                                                          <  5:389706/145‑1 (MQ=255)
                                                                          ggTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCg                                                                                                      <  2:134495/116‑1 (MQ=255)
                                                                          ggTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCg                                                                                                      >  1:134495/1‑116 (MQ=255)
                                                                              cgctcccGGCGTTCTACATCGACTTCGATGATGCGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACggg                                                                 <  4:199164/143‑1 (MQ=255)
                                                                               gCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACggg                                                                 <  8:114562/148‑1 (MQ=255)
                                                                               gCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGc                                                                <  2:356110/149‑1 (MQ=255)
                                                                                 tGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGTCCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTg                                                              >  4:231409/1‑149 (MQ=255)
                                                                                 tGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGTCCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTg                                                              >  4:236088/1‑149 (MQ=255)
                                                                                    cGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGTCCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCg                                                           <  3:236088/149‑1 (MQ=255)
                                                                                    cGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGTCCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCg                                                           <  3:231409/149‑1 (MQ=255)
                                                                                      gCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGt                                                         <  7:357587/149‑1 (MQ=255)
                                                                                              cATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGt                                                  <  7:83452/148‑1 (MQ=255)
                                                                                               aTCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTgg                                                >  3:326180/1‑149 (MQ=255)
                                                                                               aTCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTg                                                 >  1:336233/1‑148 (MQ=255)
                                                                                               aTCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTg                                                 <  2:336233/148‑1 (MQ=255)
                                                                                                          tgatgaGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCtata                                     >  6:195447/1‑149 (MQ=255)
                                                                                                                     aGTACATCAGCAACGATGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGa                            >  8:361933/1‑147 (MQ=255)
                                                                                                                     aGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGAcc                          >  7:267679/1‑149 (MQ=255)
                                                                                                                             aGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAgg                  <  1:179974/149‑1 (MQ=255)
                                                                                                                              gCAACGACGTGGGCTGTACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGGCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAggg                 <  5:119362/149‑1 (MQ=255)
                                                                                                                                aaCGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAggg                 >  2:62124/1‑147 (MQ=255)
                                                                                                                                aaCGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAggg                 <  1:62124/147‑1 (MQ=255)
                                                                                                                                              gACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAGGGGATATTCCAGGGTTc  >  1:41042/1‑148 (MQ=255)
                                                                                                                                              gACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAGGGGATATTCCAGGGTTc  >  4:335813/1‑148 (MQ=255)
                                                                                                                                              gACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAGGGGATATTCCAGGGTTc  >  5:2879/1‑148 (MQ=255)
                                                                                                                                              gACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAGGGGATATTCCAGGGTTc  >  5:208733/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGGCCAATGGCCTGGAGCCGGAAAAAGCCAAGACCTACGAAATCGGTACCCGTTATGACAATGGCGGCTTTGCCGGTGAGCTGACGGCGTTCTACATCGACTTCGATGATGAGCTGCAGTACATCAGCAACGACGTGGGCTGGACCAACCTCGGTGCGACCAAGCACCAGGGTATCGAGGCTTCGGTGCGTTATGACCTGGCCGGGCTGGACCCGCGCCTGGACGGGCTGTCGGTGAGCGGTGGCTACACCTATACCCGCGCGACCTATGAAGGGGATATTCCAGGGTTC  >  NC_002947/1006824‑1007113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑