Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,066,335 T→C 6.2% S89G (AGC→GGC)  PP_0924 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,066,3350TC 6.2% 234.7 / 4.3 81S89G (AGC→GGC) PP_0924hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/43);  new base C (3/2);  total (35/46)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

TCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTGCG  >  NC_002947/1066187‑1066483
                                                                                                                                                    |                                                                                                                                                    
tCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgct                                                                                                                                                      >  3:217164/1‑149 (MQ=255)
tCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgct                                                                                                                                                      >  8:242633/1‑149 (MQ=255)
  ggCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgctgc                                                                                                                                                    >  7:105696/1‑149 (MQ=255)
   gCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGgctgct                                                                                                                                                   >  5:241755/1‑149 (MQ=255)
          cGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTggcggc                                                                                                                                             <  6:279752/148‑1 (MQ=255)
           gCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCt                                                                                                                                           <  4:373528/149‑1 (MQ=255)
           gCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCt                                                                                                                                           <  4:379574/149‑1 (MQ=255)
            ccccAAAGCACGGCGATGGTACGGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTg                                                                                                                                          <  8:10595/149‑1 (MQ=255)
            ccccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTg                                                                                                                                          <  5:69831/149‑1 (MQ=255)
             cccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTgg                                                                                                                                         <  2:77436/149‑1 (MQ=255)
              ccAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGt                                                                                                                                        <  4:217164/149‑1 (MQ=255)
                aaaGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGa                                                                                                                                      >  5:333716/1‑149 (MQ=255)
                  aGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATc                                                                                                                                    <  2:137538/149‑1 (MQ=255)
                  aGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATc                                                                                                                                    <  8:27970/149‑1 (MQ=255)
                  aGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACAGCCCAGGCTGCTGGCGGCCTGGTGATc                                                                                                                                    <  3:155840/149‑1 (MQ=255)
                    cACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGa                                                                                                                                  <  2:305534/149‑1 (MQ=255)
                     aCGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAg                                                                                                                                 <  8:35809/149‑1 (MQ=255)
                       ggCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAg                                                                                                                                 >  6:226384/1‑147 (MQ=255)
                       ggCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCa                                                                                                                               <  3:237777/149‑1 (MQ=255)
                               tACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCGACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGggtg                                                                                                                       <  7:258065/149‑1 (MQ=255)
                               tACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGggtg                                                                                                                       >  8:139721/1‑149 (MQ=255)
                                 ccgccgTTGCGGTAGTCGGGGTCGACGCAGGTACGGCCCAGGTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGggtgg                                                                                                                      <  6:348589/148‑1 (MQ=255)
                                  cgccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGgtggtg                                                                                                                    <  8:105696/149‑1 (MQ=255)
                                   gccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGtggtgg                                                                                                                   >  3:108942/1‑149 (MQ=255)
                                   gccgTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGtggtgg                                                                                                                   <  6:301033/149‑1 (MQ=255)
                                       ttGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAAc                                                                                                               <  3:285950/149‑1 (MQ=255)
                                       ttGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAAc                                                                                                               <  5:226384/149‑1 (MQ=255)
                                          cGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCa                                                                                                             <  2:323684/148‑1 (MQ=255)
                                                  tggggCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCg                                                                                                     <  8:14857/147‑1 (MQ=255)
                                                  gggggCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAAc                                                                                                               <  1:167779/138‑1 (MQ=255)
                                                  gggggCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAAc                                                                                                               >  2:167779/1‑138 (MQ=255)
                                                  gggggCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCgg                                                                                                    >  7:287611/1‑149 (MQ=255)
                                                    gggCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTg                                                                                                  >  4:7385/1‑149 (MQ=255)
                                                         aCGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAg                                                                                             <  7:142389/149‑1 (MQ=255)
                                                           gCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGt                                                                                           >  5:258162/1‑149 (MQ=255)
                                                           gCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGt                                                                                           <  6:123729/149‑1 (MQ=255)
                                                               gTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGt                                                                                                   >  2:15414/1‑137 (MQ=255)
                                                               gTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGt                                                                                                   <  1:15414/137‑1 (MQ=255)
                                                               gTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTcgcg                                                                                       <  3:7385/149‑1 (MQ=255)
                                                                tACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTcgcg                                                                                       >  2:169012/1‑148 (MQ=255)
                                                                   ggCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTaca                                                                                   <  8:287611/149‑1 (MQ=255)
                                                                             gAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggc                                                                         >  8:361651/1‑149 (MQ=255)
                                                                             gAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggc                                                                         <  5:183986/149‑1 (MQ=255)
                                                                             gAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggc                                                                         >  1:358021/1‑149 (MQ=255)
                                                                             gAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGACCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggc                                                                         >  7:322959/1‑149 (MQ=255)
                                                                                gATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTggcggc                                                                      <  6:333716/149‑1 (MQ=255)
                                                                                 aTCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCa                                                                     <  4:108942/149‑1 (MQ=255)
                                                                                    gggCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAg                                                                    <  6:241755/147‑1 (MQ=255)
                                                                                       ccTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAc                                                               >  2:372393/1‑149 (MQ=255)
                                                                                       ccTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAc                                                               <  7:254815/149‑1 (MQ=255)
                                                                                        cTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTAACGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcg                                                              >  1:311306/1‑149 (MQ=255)
                                                                                         ttGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgt                                                             >  7:308516/1‑149 (MQ=255)
                                                                                         ttGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgt                                                             >  4:175349/1‑149 (MQ=255)
                                                                                           tcAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCATCGATGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCTAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                            <  7:294523/147‑1 (MQ=255)
                                                                                               cTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAgt                                                       >  6:76554/1‑149 (MQ=255)
                                                                                                tGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAgtc                                                      >  5:350419/1‑149 (MQ=255)
                                                                                                   aaCAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAgtcgtc                                                   <  6:258162/149‑1 (MQ=255)
                                                                                                    aCAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTcc                                                  <  2:358021/149‑1 (MQ=255)
                                                                                                        gCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCAt                                                <  8:262076/147‑1 (MQ=255)
                                                                                                              cAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTAGTAGTCGTCCATGTCCAGGc                                        >  8:346924/1‑149 (MQ=255)
                                                                                                                     aaTTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTc                                 >  5:72742/1‑149 (MQ=255)
                                                                                                                     aaTTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTc                                 <  8:308516/149‑1 (MQ=255)
                                                                                                                               cGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCt                          <  1:169012/146‑1 (MQ=255)
                                                                                                                                gCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCTCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCAATGTCCAGGCCATGCTCGGCACCttata                      >  8:306739/1‑146 (MQ=255)
                                                                                                                                gCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTcc                                                  >  5:124555/1‑121 (MQ=255)
                                                                                                                                gCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTcc                                                  <  6:124555/121‑1 (MQ=255)
                                                                                                                                gCTGTAGAAACGGCCCAGGCGGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGa                      <  6:167858/149‑1 (MQ=255)
                                                                                                                                 cTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAg                     >  7:271284/1‑149 (MQ=255)
                                                                                                                                 cTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAg                     >  5:54213/1‑149 (MQ=255)
                                                                                                                                  tGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGc                    >  5:252334/1‑149 (MQ=255)
                                                                                                                                  tGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGc                    >  6:251277/1‑149 (MQ=255)
                                                                                                                                    tAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCtt                  <  6:72742/149‑1 (MQ=255)
                                                                                                                                      gAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCCCCTTTGAGCTTgg                >  7:161098/1‑149 (MQ=255)
                                                                                                                                      gAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTgg                >  6:880/1‑149 (MQ=255)
                                                                                                                                      gAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTgg                >  1:381003/1‑149 (MQ=255)
                                                                                                                                      gAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTg                 <  5:76554/148‑1 (MQ=255)
                                                                                                                                       aaaCGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGc               >  3:208053/1‑149 (MQ=255)
                                                                                                                                          atcaccAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                            <  5:230024/97‑1 (MQ=255)
                                                                                                                                          atcaccAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                            <  5:235325/97‑1 (MQ=255)
                                                                                                                                          atcaccAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                            >  6:235325/5‑101 (MQ=255)
                                                                                                                                          atcaccAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCAcgtc                                                            >  6:230024/5‑101 (MQ=255)
                                                                                                                                           tcaccAGGCCGCCAGCAGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCtt            >  7:23508/4‑148 (MQ=255)
                                                                                                                                            gCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGa          <  7:139721/149‑1 (MQ=255)
                                                                                                                                             cccAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGGCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGaa         <  3:175349/149‑1 (MQ=255)
                                                                                                                                              ccAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTATACCAATGTGGCGGCAGTGCACCTCGTAGTCGTCCATGTCCAGGCCCTGCTCGTCACCTTTGAGCTTGGCCTTGAAt        >  7:297817/1‑149 (MQ=255)
                                                                                                                                                   ctgctgGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTgc   <  1:92634/149‑1 (MQ=255)
                                                                                                                                                    tgctgGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTgcg  <  8:225069/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCGGCCAGTTCGCCCCAAAGCACGGCGATGGTACCGCCGTTGCGGTAGTCGGGGGCGACGCAGGTACGGCCCAGTTCGAGGATCGGGCCTTGCAGCTGCAACAGGCCGTGCAGGCTGAATTCTTCTTCGCTGTAGAAACGGCCCAGGCTGCTGGCGGCCTGGTGATCGAGCAGGCGGGTGGTGGCAACCAGTTCGCCGGTGCTCAGGTCGCGTACACCAATGTGGCGGCAGTGCACGTCGTAGTCGTCCATGTCCAGGCCCTGCTCGGCACCTTTGAGCTTGGCCTTGAATTCTGCG  >  NC_002947/1066187‑1066483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑