Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,111,310	T→A	8.7%	intergenic (+31/‑79)	PP_0970 → / → PP_0971	cyanate MFS transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,111,310	0	T	A	8.7%	129.5 / 3.4	46	intergenic (+31/‑79)	PP_0970/PP_0971	cyanate MFS transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGC  >  NC_002947/1111164‑1111456                                                                                                                                                   |                                                                                                                                                       ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                                      <  1:16245/149‑1 (MQ=255)  cggcggCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                                      >  3:321154/1‑148 (MQ=255)    gcggcTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCT‑GGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgcc                                                                                                                                                     >  6:20076/1‑146 (MQ=255)           gACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCg                                                                                                                                            <  3:90684/149‑1 (MQ=255)           gACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGGTGGCGCCGTGGTGTTCGGTCTGGGGGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGc                                                                                                                                             <  2:187388/148‑1 (MQ=255)            aCGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCgg                                                                                                                                           <  5:147007/149‑1 (MQ=255)              ggCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGTCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCggg                                                                                                                                          >  6:225214/1‑148 (MQ=255)                     ggTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGGTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTgg                                                                                                                                     <  2:349336/146‑1 (MQ=255)                      gTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCGTGCCAAGAGGGCGGGACTGGCAt                                                                                                                                  >  6:192443/1‑148 (MQ=255)                      gTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCAt                                                                                                                                  >  1:21566/1‑148 (MQ=255)                      gTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACGGGa                                                                                                                                         <  3:136988/141‑1 (MQ=39)                      gTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACGGGa                                                                                                                                         >  4:136988/1‑141 (MQ=39)                          gATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGGGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCGTGCCAAGAGGGCGGGACTGGCATAAGcc                                                                                                                             <  5:192443/149‑1 (MQ=255)                                 gCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCggg                                                                                                                       <  4:301156/148‑1 (MQ=255)                                  cGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGc                                                                                                                     <  6:389358/149‑1 (MQ=255)                                     tGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGTATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgggg                                                                                                                      >  7:105267/1‑145 (MQ=255)                                     tGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg                                                                                                                                           <  5:75824/124‑1 (MQ=38)                                     tGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg                                                                                                                                           >  6:75824/1‑124 (MQ=38)                                       cTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGAt                                                                                                                 >  6:88099/1‑148 (MQ=255)                                          ggCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATc                                                                                                             <  3:152695/149‑1 (MQ=255)                                          ggCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATc                                                                                                             <  4:257296/149‑1 (MQ=255)                                            cGTTGGCGCCGTGGTGTTCGGGCT‑GGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑g                                                                                                        <  5:20076/149‑1 (MQ=255)                                                 gcgcCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGt                                                                                                    >  2:5644/1‑149 (MQ=255)                                                     cGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTc                                                                                                <  5:88099/149‑1 (MQ=255)                                                         gtgtTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAAc                                                                                            <  5:38687/149‑1 (MQ=255)                                                                   tGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑ggacgga                                                                                <  4:321154/149‑1 (MQ=255)                                                                     ggTGCCGGGCGGGCGCTGCATGTGCAGTTGACCTGCGAAAAAGTCTGAGGCGTGTTTTAGCAGTCCCGTCCTCGTGCCTGACAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑ggacgga                                                                                <  4:147634/147‑1 (MQ=255)                                                                              cgggcgCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCaa                                                                     <  5:225214/149‑1 (MQ=255)                                                                               tgtgtcTGCATGTGCATGTTACCTGTGAAAAAGTCTGATGCCTGTTTTTGCAGTCCCGTCCTCGTGCCTGGCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCaa                                                                     <  4:248643/143‑1 (MQ=255)                                                                               gggcgCTGGATGTGCTGGTGACGTGCGAAAAAGTCTGAGGCGTGTTTTAGCAGTGCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATTAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAAt                                                                    <  4:272006/149‑1 (MQ=255)                                                                                  cgcTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAg                                                                 <  3:301487/149‑1 (MQ=255)                                                                                   gcTGCATGTGCAGGTGACCTGTGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑A‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCaa                                                                     >  3:248643/1‑144 (MQ=255)                                                                                       cATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATg                                                            >  4:52294/1‑149 (MQ=255)                                                                                             cAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAAcc                                                      >  8:168010/1‑149 (MQ=255)                                                                                                   aCCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGcc                                                 >  1:294990/1‑148 (MQ=255)                                                                                                    ccTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCt                                               <  8:105267/149‑1 (MQ=255)                                                                                                      tGCGAACAAGTCTGTGGCGTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGccc                                                <  6:57395/146‑1 (MQ=255)                                                                                                      tGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGccc                                                >  5:57395/1‑146 (MQ=255)                                                                                                        cGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGAt                                            <  3:52294/148‑1 (MQ=255)                                                                                                          aaaaaGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCAc                                         >  1:348524/1‑149 (MQ=255)                                                                                                           aaaaGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgg                                                                                                                        >  7:285640/1‑73 (MQ=255)                                                                                                                       ccTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAg                            >  6:360096/1‑149 (MQ=255)                                                                                                                       ccTGTTTTAGCAGACCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTTTCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAg                            >  6:9215/1‑149 (MQ=255)                                                                                                                         tGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGc                          >  2:366429/1‑149 (MQ=255)                                                                                                                          gTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGATGGCGGTTTTCGAACCTAAGGGACGGACCAGCCCCCAATGAGTGATGCCCACCGCGCCCTGATCACCCGTTTCTAc                               >  1:315583/1‑147 (MQ=255)                                                                                                                                    tCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGcc               <  2:21566/149‑1 (MQ=255)                                                                                                                                      ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTg             >  4:336856/1‑149 (MQ=255)                                                                                                                                      ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTg             >  3:270995/1‑149 (MQ=255)                                                                                                                                       cGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTg             >  1:370821/1‑148 (MQ=255)                                                                                                                                                  ctgccAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGc  >  8:227027/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCG‑T‑GCAGTTTTCGAACCTA‑‑GGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGC  >  NC_002947/1111164‑1111456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑