Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,132,532 C→T 5.0% A29T (GCA→ACA)  PP_0993 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,132,5320CT 5.0% 240.3 / 2.0 79A29T (GCA→ACA) PP_0993hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/35);  new base T (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

AGGTGCCGGCGGGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGTGC  >  NC_002947/1132385‑1132676
                                                                                                                                                       |                                                                                                                                                 
aGGTGCCGGCGGGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCg                                                                                                                                                  >  3:12233/1‑149 (MQ=255)
     ccGGCGGGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGtt                                                                                                                                              <  5:168292/148‑1 (MQ=255)
     ccGGCGGGGACGGTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGtt                                                                                                                                              <  4:197220/148‑1 (MQ=255)
      cggcggGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGc                                                                                                                                            <  8:83271/149‑1 (MQ=255)
      cggcggGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGc                                                                                                                                            <  3:247692/149‑1 (MQ=255)
        gcggGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑Gt                                                                                                                                         <  4:328031/149‑1 (MQ=255)
        gcggGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑Gt                                                                                                                                         <  2:377743/149‑1 (MQ=255)
            ggACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTg                                                                                                                                     <  4:12233/149‑1 (MQ=255)
             gACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGt                                                                                                                                    >  4:287078/1‑149 (MQ=255)
                gTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCgg                                                                                                                                 >  2:169613/1‑149 (MQ=255)
                 tttttCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGgc                                                                                                                                <  5:317591/149‑1 (MQ=255)
                       cAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTGGTAGACATGC‑CATGGCGAGCGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGgccgc                                                                                                                             >  1:315076/1‑149 (MQ=255)
                        aGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGCGGGGGAGTCGgccgcc                                                                                                                            >  1:328327/1‑147 (MQ=255)
                           gCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATggcg                                                                                                                      >  7:335791/1‑149 (MQ=255)
                             aTGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATg                                                                                                                         <  7:254447/144‑1 (MQ=255)
                             aTGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATg                                                                                                                         >  8:254447/1‑144 (MQ=255)
                               gCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGTGGGTTGC‑GTGCTGTCGGCCGCCATGGCGgcgc                                                                                                                  >  8:249777/1‑149 (MQ=255)
                               gCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGTGGGTTGC‑GTGCTGTCGGCCGCCATGGCGgcgc                                                                                                                  >  4:163930/1‑149 (MQ=255)
                               gCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCACGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGgcgc                                                                                                                  >  7:2848/1‑149 (MQ=255)
                                ccGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATggcggccga                                                                                                                 <  7:184477/149‑4 (MQ=255)
                                 cgtcgtAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCACTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCt                                                                                                                >  6:124897/1‑149 (MQ=255)
                                   tcgtAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCACTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGc                                                                                                              <  5:124897/149‑1 (MQ=255)
                                            ttcttcGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTgg                                                                                                      >  4:177443/1‑148 (MQ=255)
                                            ttcttcGCACTGCCAGCGCAGCTGCTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGACGCCATGGCGGCGCCTGCAAGCGTgg                                                                                                      >  5:86290/1‑148 (MQ=255)
                                               ttcGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAg                                                                                                  >  3:193261/1‑149 (MQ=255)
                                                 cGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGCGCATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAgc                                                                                                 >  1:101400/1‑149 (MQ=255)
                                                 cGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAgcg                                                                                                >  4:306839/1‑149 (MQ=255)
                                                  gCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAgcgc                                                                                               >  5:152139/1‑149 (MQ=255)
                                                  gCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAgcgc                                                                                               <  7:12356/149‑1 (MQ=255)
                                                   cACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGcgcg                                                                                              >  7:183312/1‑149 (MQ=255)
                                                      tGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGt                                                                                           >  2:82394/1‑149 (MQ=255)
                                                       gCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGtg                                                                                          >  1:70230/1‑149 (MQ=255)
                                                        ccAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCCAGCGCGAGtgc                                                                                         >  8:304430/1‑149 (MQ=255)
                                                        ccAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGtgc                                                                                         >  4:333173/1‑149 (MQ=255)
                                                          aGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGtgctg                                                                                       >  1:358440/1‑149 (MQ=255)
                                                            cgcAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GGGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCg                                                                                     <  7:70037/149‑1 (MQ=255)
                                                                    gTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAg                                                                             >  8:19106/1‑149 (MQ=255)
                                                                    gTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAg                                                                             >  5:210076/1‑149 (MQ=255)
                                                                     tGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAgg                                                                            <  8:335791/149‑1 (MQ=255)
                                                                      gccggcgccggcCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAggg                                                                           >  3:61727/1‑149 (MQ=255)
                                                                       ccggcgccggcCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAggg                                                                           <  3:341362/148‑1 (MQ=255)
                                                                               ggcCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGttt                                                                  >  5:207094/1‑149 (MQ=255)
                                                                               ggcCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGttt                                                                  <  6:74208/149‑1 (MQ=255)
                                                                               ggcCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGttt                                                                  <  1:169613/149‑1 (MQ=255)
                                                                               ggcCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCACGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGTGGTGGTGAGGGCGAGGGttt                                                                  <  8:2848/149‑1 (MQ=255)
                                                                                  cTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCa                                                               >  2:248252/1‑149 (MQ=255)
                                                                                     gCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCAt                                                              <  5:95960/147‑1 (MQ=255)
                                                                                            ttCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCggt                                                                                   >  8:266408/1‑119 (MQ=255)
                                                                                            ttCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCggt                                                                                   <  7:266408/119‑1 (MQ=255)
                                                                                            ttCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAAtct                                                     >  5:245061/1‑149 (MQ=255)
                                                                                               tt‑‑tCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCt                                                  >  2:101060/1‑149 (MQ=255)
                                                                                                   tCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCt                                                  <  6:152139/147‑1 (MQ=255)
                                                                                                     cTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGTGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTgata                                              <  7:249777/149‑3 (MQ=255)
                                                                                                     cTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGTGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTgaga                                              <  3:163930/149‑1 (MQ=255)
                                                                                                     cTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTgaga                                              <  4:193261/149‑1 (MQ=255)
                                                                                                     cTGC‑CATGGCGA‑CGCCGTAGCA‑TTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGagag                                             >  5:119039/1‑149 (MQ=255)
                                                                                                       gC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAgt                                            >  5:98569/1‑149 (MQ=255)
                                                                                                        c‑cATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAg                                                                       >  1:206746/1‑121 (MQ=255)
                                                                                                        c‑cATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAg                                                                       <  2:206746/121‑1 (MQ=255)
                                                                                                          cATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAgtgt                                          <  6:289552/149‑1 (MQ=255)
                                                                                                           aTGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGtgtg                                         >  3:1136/1‑149 (MQ=255)
                                                                                                              gCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGcc                                      >  3:144990/1‑149 (MQ=255)
                                                                                                                gA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGt                                    >  4:170724/1‑149 (MQ=255)
                                                                                                                   cGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTgg                                  <  2:358440/149‑1 (MQ=255)
                                                                                                                   cGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTgg                                  <  8:183312/149‑1 (MQ=255)
                                                                                                                    gCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGt                                 >  3:334643/1‑149 (MQ=255)
                                                                                                                      cGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCt                               <  6:210076/149‑1 (MQ=255)
                                                                                                                            aTTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACcg                         >  8:331343/1‑149 (MQ=255)
                                                                                                                                   cATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACcgg                        <  7:44962/143‑1 (MQ=255)
                                                                                                                                   cATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACcgg                        >  8:44962/1‑143 (MQ=255)
                                                                                                                                       gCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATg               <  6:292800/148‑1 (MQ=255)
                                                                                                                                       gCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGGGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATg               <  3:287078/148‑1 (MQ=255)
                                                                                                                                        cAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATg               >  6:211048/1‑147 (MQ=255)
                                                                                                                                               aTTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTc      <  4:300603/149‑1 (MQ=255)
                                                                                                                                                  gCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGt    <  6:98569/148‑1 (MQ=255)
                                                                                                                                                  gCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGt    <  1:248252/148‑1 (MQ=255)
                                                                                                                                                  gCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGTg   >  4:155109/1‑149 (MQ=255)
                                                                                                                                                   ccTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGTGc  <  5:211048/149‑1 (MQ=255)
                                                                                                                                                   ccTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGTGc  <  3:177443/149‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
AGGTGCCGGCGGGGACGTTTTTCCAGTGCATGCCGTCGTAGTCCTTCTTCGCACTGCCAGCGCAGCTGGTGCCGGCGCCGGCCTTGCAATCGTTCTT‑‑TCCTGC‑CATGGCGA‑CGCCGTAGCATTTTTCCATGGCAGCGCCATTGCCTGCGGGTTGC‑GTGCTGTCGGCCGCCATGGCGGCGCCTGCAAGCGTGGCAAGCGCGAGTGCTGCGGTGGTGAGGGCGAGGGTTTTCATGGTCAATCTCCTGAGAGTGTGGCCGTGGTCTATACCGGCGGCTCATGGAGTAATTCGTGC  >  NC_002947/1132385‑1132676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑