Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,158,930 A→G 6.2% M355V (ATG→GTG) ‡ gtsA → mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,158,9300AG 6.2% 233.9 / 4.7 80M355V (ATG→GTG) ‡gtsAmannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (38/37);  new base G (3/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

TGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGATGTGGTGACCAA  >  NC_002947/1158793‑1159078
                                                                                                                                         |                                                                                                                                                    
tggtgTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGAAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTGTTCAGCATCAACAAGGGGTCGATCCCGGTACGCAACGCCTTGCTTGCCGACATGGGCAAGTAt                                                                                                                                           >  2:351186/1‑149 (MQ=255)
     ttCAAGCATAACAACGCTGCCACCTCTGCTGGTCAGAAGTAAATCGCCCGCAAGGTGTTGGGTTAGGAATTCCAGACGGTCTTCAGCATCAACAAGGTTACGATCCCGGTACGCAACGACATACTTGCCCATGTCGGCAAGCATgtcg                                                                                                                                       <  1:286846/148‑4 (MQ=255)
     ttCAAGCAGAACAACGCTGGCACCTCTGCTTGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGATGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATTGGCAAGTATGGttt                                                                                                                                      <  2:290868/149‑1 (MQ=255)
     ttCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGttt                                                                                                                                      >  7:309325/1‑149 (MQ=255)
     ttCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGttt                                                                                                                                      <  3:191775/149‑1 (MQ=255)
     ttCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATACTTGCCCATGTCGGCAAGCATgtcg                                                                                                                                       <  5:353435/148‑4 (MQ=255)
     ttCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAACGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGCGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGCGCAAGTATCGttt                                                                                                                                      >  8:195817/1‑149 (MQ=255)
       cAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGGTGGGTTAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCGCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTc                                                                                                                                     <  7:195817/148‑1 (MQ=255)
          gCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATg                                                                                                                                 >  8:59811/1‑149 (MQ=255)
           cAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGc                                                                                                                                <  1:206953/149‑1 (MQ=255)
             gAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCt                                                                                                                              <  8:64634/149‑1 (MQ=255)
               acaacGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCtgt                                                                                                                            <  6:122602/149‑1 (MQ=255)
                 aacGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGc                                                                                                                          <  3:132435/149‑1 (MQ=255)
                 aacGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGc                                                                                                                          >  8:213902/1‑149 (MQ=255)
                     cTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCaa                                                                                                                      <  7:305407/149‑1 (MQ=255)
                        gCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCaaa                                                                                                                     <  8:256700/147‑1 (MQ=255)
                          aCCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGCCATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTc                                                                                                                 >  2:167287/1‑149 (MQ=255)
                           ccactgctgGTGAGCAGCACATCGCCCCCAAGGTGGTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACTAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATTGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTcc                                                                                                                <  8:361255/146‑1 (MQ=255)
                           ccTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTcc                                                                                                                <  8:212050/149‑1 (MQ=255)
                                  tgGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAg                                                                                                          >  2:347648/1‑148 (MQ=255)
                                    gTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGAc                                                                                                       >  2:381504/1‑149 (MQ=255)
                                    gTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGAc                                                                                                       >  7:151891/1‑149 (MQ=255)
                                      cagcagGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACtt                                                                                                     >  2:85874/1‑149 (MQ=255)
                                       agcagGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTc                                                                                                    <  6:329128/149‑1 (MQ=255)
                                          agGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTg                                                                                                 <  8:309325/149‑1 (MQ=255)
                                          agGACATCGCCAGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTg                                                                                                 <  3:282896/149‑1 (MQ=255)
                                                tCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGAc                                                                                           >  5:392591/1‑149 (MQ=255)
                                                tCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGAc                                                                                           >  8:311236/1‑149 (MQ=255)
                                                    ccGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCGCAAACCACCGCCAAGGACTTCCTGGCTGACGcc                                                                                        >  7:55853/1‑148 (MQ=255)
                                                                gggTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGggcggc                                                                            <  5:214792/148‑1 (MQ=255)
                                                                gggTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGcc                                                                           >  8:383/1‑149 (MQ=255)
                                                                 ggTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCt                                                                          >  3:185216/1‑149 (MQ=255)
                                                                 ggTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCAAAAACGGGCGGCCt                                                                          <  8:151891/149‑1 (MQ=255)
                                                                    gAGGACTTCCAGAAGGTCTTCAGCATCAACAA‑GGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCaa                                                                      <  5:196560/149‑1 (MQ=255)
                                                                      ggACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAAc                                                                     >  1:233114/1‑149 (MQ=255)
                                                                         cTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAAc                                                                                   <  5:104545/132‑1 (MQ=255)
                                                                         cTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAAc                                                                                   >  6:104545/1‑132 (MQ=255)
                                                                         cTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAAcc                                                                    >  1:115957/1‑147 (MQ=255)
                                                                         cTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGa                                                                  <  2:115957/149‑1 (MQ=255)
                                                                          ttCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAg                                                                 >  6:332358/1‑149 (MQ=255)
                                                                            ccAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCa                                                               >  8:246452/1‑149 (MQ=255)
                                                                               gAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATgg                                                            <  1:347648/149‑1 (MQ=255)
                                                                                  ggTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGgcg                                                          >  5:375056/1‑148 (MQ=255)
                                                                                    tCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTgg                                                                                                >  6:154159/1‑108 (MQ=255)
                                                                                    tCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTgg                                                                                                <  5:154159/108‑1 (MQ=255)
                                                                                    tCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGca                                                        >  3:84092/1‑148 (MQ=255)
                                                                                     cTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGTCCTGCAACCGAGCATGGCGcaca                                                      <  7:59811/149‑1 (MQ=255)
                                                                                      ttCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCacaa                                                     >  6:104693/1‑149 (MQ=255)
                                                                                          gCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATg                                                 >  1:92097/1‑149 (MQ=255)
                                                                                            aTCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGACTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGc                                               >  6:294125/1‑149 (MQ=255)
                                                                                             tCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGcc                                              <  2:233114/149‑1 (MQ=255)
                                                                                             tCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGcc                                              <  5:104693/149‑1 (MQ=255)
                                                                                             tCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGc                                               >  3:138006/1‑148 (MQ=255)
                                                                                                gcaaGGGTGCGATCCCGGTAGGCAGCGACATGCTTGCCGACATGGGGATGTATGGTTTCGATGCCTGTGCTGAGACCTCCGCCAGGGAGTTCCTGGCTGACGCCAAGCCGGGCGGCCTGCAACCGAGCATGGCGGACAACATGGccac                                            >  4:54518/2‑148 (MQ=255)
                                                                                                acaaGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGccacc                                           >  7:252544/1‑149 (MQ=255)
                                                                                                acaaGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGccac                                            >  8:213903/1‑148 (MQ=255)
                                                                                                  aaGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCcaccac                                         >  3:337423/1‑149 (MQ=255)
                                                                                                     ggTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCt                                      >  2:111149/1‑149 (MQ=255)
                                                                                                         cGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGc                                   <  7:213902/148‑1 (MQ=255)
                                                                                                          gATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGcc                                  <  6:375056/148‑1 (MQ=255)
                                                                                                          gATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGcc                                  <  8:252544/148‑1 (MQ=255)
                                                                                                           aTCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGt                                <  7:383/149‑1 (MQ=255)
                                                                                                              ccGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCa                             <  2:92097/149‑1 (MQ=255)
                                                                                                              ccGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCa                             <  4:185216/149‑1 (MQ=255)
                                                                                                               cGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAg                            >  4:10139/1‑149 (MQ=255)
                                                                                                               cGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAg                            >  2:181441/1‑149 (MQ=255)
                                                                                                                  tACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGgc                         <  5:332358/149‑1 (MQ=255)
                                                                                                                     gCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGcgcg                      <  7:213903/149‑1 (MQ=255)
                                                                                                                      cAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTAACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGt                     >  4:54603/1‑149 (MQ=255)
                                                                                                                       aaCGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGtt                    >  7:205374/1‑149 (MQ=255)
                                                                                                                        aCGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGttc                   <  6:392591/149‑1 (MQ=255)
                                                                                                                          gACATGCTTGCCGACAGGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGttctt                 >  7:331019/1‑149 (MQ=255)
                                                                                                                          accATACTTGCCCATGTCGGCAAGCATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGttctt                 >  2:18878/3‑149 (MQ=255)
                                                                                                                            cATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCg               <  1:381504/149‑1 (MQ=255)
                                                                                                                            cATACTTGCCCATGTCGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACACCATGGCCACCACGCTGGCCGTGCAGGGCGCGttcttc                >  7:265859/1‑148 (MQ=255)
                                                                                                                            cATACTTGCCCATGTCGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGttcttc                >  7:270883/1‑148 (MQ=255)
                                                                                                                              tGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGAt             <  7:311236/149‑1 (MQ=255)
                                                                                                                              tGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGACTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGAt             <  5:294125/149‑1 (MQ=255)
                                                                                                                               gCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGAtg            >  3:140723/1‑149 (MQ=255)
                                                                                                                                   gCCGACATGGGCAAGTATGGTTTCGATGCCTGTTCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGAtgtg          <  7:150424/147‑1 (MQ=255)
                                                                                                                                         aTGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGATGTGGTGACCaa  >  6:88703/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                    
TGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTTGCCGACATGGGCAAGTATGGTTTCGATGCCTGTGCTCAAACCTCCGCCAAGGACTTCCTGGCTGACGCCAAAACGGGCGGCCTGCAACCGAGCATGGCGCACAACATGGCCACCACGCTGGCCGTGCAGGGCGCGTTCTTCGATGTGGTGACCAA  >  NC_002947/1158793‑1159078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑