Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	160,310	C→T	7.7%	E27K (GAG→AAG)	PP_0149 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	160,310	0	C	T	7.7%	227.9 / 6.1	79	E27K (GAG→AAG)	PP_0149	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/40);  new base T (4/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

GTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGG  >  NC_002947/160164‑160457                                                                                                                                                   |                                                                                                                                                    gTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTc                                                                                                                                                     >  7:202079/1‑147 (MQ=255)    ggcaggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCt                                                                                                                                                >  6:66418/1‑149 (MQ=255)      caggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTg                                                                                                                                               >  6:220775/1‑148 (MQ=255)      caggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTg                                                                                                                                               >  6:143052/1‑148 (MQ=255)         gcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAgg                                                                                                                                           >  7:188700/1‑149 (MQ=255)         gcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAgg                                                                                                                                           >  6:375896/1‑149 (MQ=255)          caTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAggc                                                                                                                                          <  5:220775/149‑2 (MQ=255)          caTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGt                                                                                                                                          <  3:352383/149‑1 (MQ=255)                gtCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGcc                                                                                                                                     >  6:234755/1‑148 (MQ=255)                   gCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAg                                                                                                                                            <  4:280038/138‑1 (MQ=255)                   gCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAg                                                                                                                                            >  3:280038/1‑138 (MQ=255)                     tCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcg                                                                                                                               >  3:159323/1‑149 (MQ=255)                      ccAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcgg                                                                                                                              <  5:375896/149‑1 (MQ=255)                       cAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAggcg                                                                                                                               <  7:47928/147‑1 (MQ=255)                          cTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGt                                                                                                                          <  2:314995/149‑1 (MQ=255)                          cTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGt                                                                                                                          <  2:51490/149‑1 (MQ=255)                             aCCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTg                                                                                                                         <  5:204282/147‑1 (MQ=255)                                     gcggcgGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCg                                                                                                               <  5:310414/149‑1 (MQ=255)                                        gcggTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGt                                                                                                              >  1:150555/1‑147 (MQ=255)                                          ggTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAgg                                                                                                          <  8:188700/149‑1 (MQ=255)                                               aGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGgcg                                                                                                       >  7:190027/1‑147 (MQ=255)                                                gCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGt                                                                                                              <  3:101689/139‑1 (MQ=255)                                                gCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGt                                                                                                              >  4:101689/1‑139 (MQ=255)                                                  tGTTGCTTGAAGCGCGGTTGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGGAGGTCGGCCAAGGCGGGCGTGCCGAGCTGGGTCAGGGCGCAGc                                                                                                   <  2:101113/148‑1 (MQ=255)                                                    ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCCAGGCGGGCGTGCCGCGCAGCGTCAGGGCGCAGCCaa                                                                                                >  1:386747/1‑149 (MQ=255)                                                        ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                    <  6:359475/125‑1 (MQ=255)                                                        ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGag                                                                                                                    >  5:359475/1‑125 (MQ=255)                                                        ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcag                                                                                            >  6:163658/1‑149 (MQ=255)                                                        ttGAAGCGCGGTGGCAGCTTGCCAATGGCCTCGTCGAGCAGGGCATGGGAGGCTTTAGTTTGTTGTGGGTAGAGGCCGGACGCCTGCAGCTGAAGCTGCATGTAGGCCAAAGCCGGGGTGCGCGGCAGCGTCCGGGCGCAGGCCagccg                                                                                            >  8:132766/1‑147 (MQ=255)                                                              cgcgGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgc                                                                                      <  8:202079/149‑1 (MQ=255)                                                              cgcgGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgc                                                                                      >  4:374351/1‑149 (MQ=255)                                                                cgGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcac                                                                                    <  3:111202/149‑1 (MQ=255)                                                                  gTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgc                                                                                  <  5:279397/149‑1 (MQ=255)                                                                    ggCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgcac                                                                                <  7:289610/149‑1 (MQ=255)                                                                    ggCAGCGTGCCCATGGCCTCGTCGAGCAGGGCGTGGGAGGCTTTAGTTTGTTGGGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgcac                                                                                <  7:160997/149‑1 (MQ=255)                                                                      cAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCacgcacgc                                                                              <  1:228080/149‑1 (MQ=255)                                                                             cccTTGGCCTCGTCGAGCAGGGCTTGGGTGGCTTTAGTTTGTTGTGGGGCGAGGCCGGACGCGTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTcac                                                                       <  2:215824/149‑1 (MQ=255)                                                                              ccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGGCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGGCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTcaca                                                                      <  5:234755/149‑1 (MQ=255)                                                                                  ggCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                  >  4:36571/1‑149 (MQ=255)                                                                                   gCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                  <  5:163658/148‑1 (MQ=255)                                                                                   gCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTAACAGTGc                                                                  >  3:293074/1‑148 (MQ=255)                                                                                    ccTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                  >  7:276726/1‑147 (MQ=255)                                                                                    ccTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGAGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGa                                                                <  7:110578/149‑1 (MQ=255)                                                                                     cTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGGTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGat                                                               >  1:249534/1‑148 (MQ=255)                                                                                        gtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGt                                                              <  3:36571/147‑1 (MQ=255)                                                                                        gtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCAGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCGTCACAGTGCGAGTAt                                                            <  2:251102/149‑1 (MQ=255)                                                                                           gAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGc                                                         <  2:255100/149‑1 (MQ=255)                                                                                            aGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCa                                                                                                            <  6:359439/97‑1 (MQ=255)                                                                                            aGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCa                                                                                                            <  6:353800/97‑1 (MQ=255)                                                                                            aGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCa                                                                                                            >  5:353800/1‑97 (MQ=255)                                                                                            aGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCa                                                                                                            >  5:359439/1‑97 (MQ=255)                                                                                             gCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCtt                                                       <  3:374351/149‑1 (MQ=255)                                                                                               aGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCa                                                     <  5:66418/149‑1 (MQ=255)                                                                                                 ggCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGc                                                   <  8:62171/149‑1 (MQ=255)                                                                                                  gCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGcc                                                  <  5:29398/149‑1 (MQ=255)                                                                                                       ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTAt                                                            <  3:221836/134‑1 (MQ=255)                                                                                                       ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTAt                                                            >  4:221836/1‑134 (MQ=255)                                                                                                       ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATAc                                             <  5:143052/149‑1 (MQ=255)                                                                                                        gAGGCTTTAGTTTGTGGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATAGCTTCAGCCAATAcc                                            <  7:157786/149‑1 (MQ=255)                                                                                                                 gTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTg                                   >  7:191841/1‑149 (MQ=255)                                                                                                                  tttGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCACGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGcgg                                   <  2:150555/148‑3 (MQ=255)                                                                                                                   ttgttgTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCa                                                     >  1:163434/1‑129 (MQ=255)                                                                                                                   ttgttgTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCa                                                     <  2:163434/129‑1 (MQ=255)                                                                                                                      ttgtGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGgcgc                               <  8:190027/148‑1 (MQ=255)                                                                                                                               gAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTc                     <  1:235185/149‑1 (MQ=255)                                                                                                                               gAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTc                     >  5:241609/1‑149 (MQ=255)                                                                                                                                       cgaccTGCAGCTTGAGCTGCAGGCCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAGGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATCGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActc             >  2:305107/4‑149 (MQ=255)                                                                                                                                       aCGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActc             >  6:282717/1‑149 (MQ=255)                                                                                                                                        gaccTGCAGCTTGAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcg            >  6:294784/3‑149 (MQ=255)                                                                                                                                         gCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgc           >  4:226892/1‑149 (MQ=255)                                                                                                                                          ccTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCg                                      <  6:93300/121‑1 (MQ=255)                                                                                                                                          ccTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCg                                      >  5:93300/1‑121 (MQ=255)                                                                                                                                           cTGCAGCTTGAGCTGCAGGCCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGt                                                              >  2:346960/1‑96 (MQ=255)                                                                                                                                           cTGCAGCTTGAGCTGCAGGCCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGt                                                              <  1:346960/96‑1 (MQ=255)                                                                                                                                           cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc         >  2:81565/1‑149 (MQ=255)                                                                                                                                              ctgCTCAAGCGGCTGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctcg        <  6:233457/145‑1 (MQ=255)                                                                                                                                                 cTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAg   >  2:194730/1‑149 (MQ=255)                                                                                                                                                 cTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAg   >  3:26712/1‑149 (MQ=255)                                                                                                                                                 cTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAg   >  6:13744/1‑149 (MQ=255)                                                                                                                                                  tCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAgg  >  2:36977/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGG  >  NC_002947/160164‑160457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑