Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,210,829 T→A 12.0% intergenic (+19/+18) ytnA → / ← PP_1060 amino acid permease YtnA/glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,210,8290TA12.0% 105.2 / 4.1 41intergenic (+19/+18)ytnA/PP_1060amino acid permease YtnA/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (9/27);  new base A (3/2);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

TCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGC  >  NC_002947/1210689‑1210973
                                                                                                                                            |                                                                                                                                                 
tCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGccc                                                                                                                                            >  1:47212/1‑148 (MQ=255)
tCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGccc                                                                                                                                            <  2:47212/148‑1 (MQ=255)
  gAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGCCCCac                                                                                                                                         <  7:262896/149‑2 (MQ=255)
      aTACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGtt                                                                                                                                     <  1:303396/149‑1 (MQ=255)
         cgcgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTtctc                                                                                                                                  <  7:123559/149‑1 (MQ=255)
                  cccTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGgccgc                                                                                                                         <  8:47133/149‑1 (MQ=255)
                         cATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCg                                                                                                                  <  3:378995/149‑1 (MQ=255)
                           tCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCt                                                                                                                <  1:190670/149‑1 (MQ=255)
                                 cGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCgg                                                                                                          <  1:206928/149‑1 (MQ=255)
                                      ttCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGa                                                                                                     <  5:59688/149‑1 (MQ=255)
                                         cTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGaaa                                                                                                   <  3:247467/148‑1 (MQ=255)
                                         cTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGaaa                                                                                                   <  3:242460/148‑1 (MQ=255)
                                               ttGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGCATTTAGGCCCCACCGGTATCTAACGCCCCCACAAAGCAGTGAGGCTCGcaaaagtacc                                                                                            >  6:137310/1‑142 (MQ=255)
                                                   tGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGCTTTTCGGGCCCCGCGGTGTCTCACGCCCCCCCATAGGTGTGCGGCGGGAAAAA‑GTACGcccc                                                                                       >  6:277542/1‑148 (MQ=255)
                                                    gACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCgg                                                                                      <  3:248901/149‑1 (MQ=255)
                                                      cAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGc                                                                                    <  5:268501/149‑1 (MQ=255)
                                                              tATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGcc                                                                            <  5:137310/149‑1 (MQ=255)
                                                                   cacGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCATTTAGGCCCCAGCCTTAAATAACGCCCCCACATACCGGTTCGGCTCGAAAAA‑CTCCGCCCCGGCCAATTGCcccaa                                                                        >  8:211131/1‑147 (MQ=255)
                                                                          cGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGaa                                                                 <  2:12917/148‑1 (MQ=255)
                                                                                  gcCGAAAGAGCCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCGGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTgg                                                        <  5:279103/149‑1 (MQ=255)
                                                                                  gcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTgg                                                        <  3:305877/149‑1 (MQ=255)
                                                                                        aGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTc                                                  <  5:277542/149‑1 (MQ=255)
                                                                                          aCGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCGTTTCGGCCCCCCCGGTGTCTCACGCCGCCCCATAGCGGTGCGGCGCGAAACA‑GTGCGCCCCGGGCAGGTGGCCCAAACCCGCATAGAACAGGCCGTTGAc                                                >  6:187456/1‑149 (MQ=255)
                                                                                           cGCACAAGGTGGGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCAcc                                               <  6:202269/149‑1 (MQ=255)
                                                                                               caAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGTGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGc                                           <  7:332209/149‑1 (MQ=255)
                                                                                                    tgtgACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTc                                      <  2:134955/149‑1 (MQ=255)
                                                                                                             tACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCa                             <  1:373497/149‑1 (MQ=255)
                                                                                                                aGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCt                          <  5:240272/149‑1 (MQ=255)
                                                                                                                   ctcctGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGc                       <  8:268982/149‑1 (MQ=255)
                                                                                                                         acctgacGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCaa                  <  7:202794/148‑1 (MQ=255)
                                                                                                                         acctgacGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAg                 <  5:187456/149‑1 (MQ=255)
                                                                                                                               gctGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACTGGGCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAaca             >  1:116596/3‑148 (MQ=255)
                                                                                                                               gctGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAAcaca           >  3:85287/3‑149 (MQ=255)
                                                                                                                               cGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAAcaca           <  1:342393/149‑1 (MQ=255)
                                                                                                                                gTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt          >  7:181847/1‑149 (MQ=255)
                                                                                                                                gTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt          >  3:233420/1‑149 (MQ=255)
                                                                                                                                   gggCAGCTTTGCGTCCCCACCGTTCTATCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCCGGCCAGTTGCGACATCCGGGAATAGAACTGGCCGTTCAACTCCCCGGTCAGCCGCTCACCTGGCTACAAGAACACATGCa       >  6:289041/1‑149 (MQ=255)
                                                                                                                                    ggCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCAt      >  6:157283/1‑149 (MQ=255)
                                                                                                                                     gCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATc     >  5:86342/1‑149 (MQ=255)
                                                                                                                                        gCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGc  <  7:211131/149‑1 (MQ=255)
                                                                                                                                        gCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGc  <  1:76700/149‑1 (MQ=255)
                                                                                                                                          tttGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGc  >  7:190154/1‑147 (MQ=255)
                                                                                                                                          tttGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTAACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGc  >  1:180943/1‑147 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGGCCGCCACTTCGCTGTGCGGCTCGAAACT‑GTCCGCCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGC  >  NC_002947/1210689‑1210973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑