Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 160,513 A→C 5.6% F65V (TTC→GTC)  PP_0150 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947160,5130AC 5.6% 226.2 / 4.0 72F65V (TTC→GTC) PP_0150hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/41);  new base C (2/2);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

GCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGG  >  NC_002947/160367‑160659
                                                                                                                                                  |                                                                                                                                                  
gcCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGc                                                                                                                                                  <  5:280958/149‑1 (MQ=255)
    ggCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGc                                                                                                                                              <  7:74418/149‑1 (MQ=255)
       acgcacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGc                                                                                                                                           >  5:145249/1‑149 (MQ=255)
          cacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtc                                                                                                                                        >  2:197219/1‑149 (MQ=255)
          cacgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtc                                                                                                                                        >  6:328196/1‑149 (MQ=255)
           acgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGTTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcg                                                                                                                                       <  1:167002/149‑1 (MQ=255)
              cgcTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcg                                                                                                                                    >  5:295438/1‑149 (MQ=255)
               gcTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                   >  1:213552/1‑149 (MQ=255)
               gcTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                   >  7:319695/1‑149 (MQ=255)
               gcTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                   >  5:103096/1‑149 (MQ=255)
                cTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTg                                                                                                                                  <  1:13556/149‑1 (MQ=255)
                cTTAACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtcgcc                                                                                                                                  <  4:293074/149‑3 (MQ=255)
                       gTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTcg                                                                                                                           <  5:170031/149‑1 (MQ=255)
                               aTGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGc                                                                                                                                                  <  1:48948/118‑1 (MQ=255)
                               aTGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGc                                                                                                                                                  >  2:48948/1‑118 (MQ=255)
                                 ggCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCAACACtt                                                                                                                 >  4:199719/1‑149 (MQ=255)
                                  gCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACttt                                                                                                                <  5:328196/149‑1 (MQ=255)
                                  gCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACttt                                                                                                                <  1:197219/149‑1 (MQ=255)
                                   cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                               >  5:171728/1‑149 (MQ=255)
                                      cAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGa                                                                                                             <  2:135194/148‑1 (MQ=255)
                                       aGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGt                                                                                                           <  8:78980/149‑1 (MQ=255)
                                         ccAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcg                                                                                                         >  5:168640/1‑149 (MQ=255)
                                         ccAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcg                                                                                                         >  7:29604/1‑149 (MQ=255)
                                          cAATACCTGGTCGCTGGCGCCTCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACttt                                                                                                                <  6:207236/141‑1 (MQ=255)
                                          cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTgagctcg                                                                                                         >  5:207236/1‑142 (MQ=255)
                                          cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                        <  1:347724/149‑1 (MQ=255)
                                          cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                        <  6:336715/149‑1 (MQ=255)
                                          cAATACCTGGGCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCTGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                        <  8:191841/149‑1 (MQ=255)
                                                cTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATc                                                                                                   >  6:243480/1‑148 (MQ=255)
                                                   gTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAgg                                                                                               >  8:142355/1‑149 (MQ=255)
                                                          gcgcCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTGTTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtggt                                                                                        <  7:306513/149‑1 (MQ=255)
                                                          gcgcCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtggt                                                                                        <  2:24222/149‑1 (MQ=255)
                                                            gccgcGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGgtgt                                                                                      <  2:249534/149‑1 (MQ=255)
                                                            gccgcGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGgtgt                                                                                      >  7:195390/1‑149 (MQ=255)
                                                             ccgcGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTc                                                                                     <  4:26712/149‑1 (MQ=255)
                                                              cgcgcgCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCt                                                                                    <  5:180173/149‑1 (MQ=255)
                                                                ggcgcTTCCGCTACTCGCTCGCGCAGGGTTTTGAAGGCCGCCTCGATTTGTACGGCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTTGTGGTGTCTGa                                                                                  <  7:132766/148‑1 (MQ=255)
                                                                 gcgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCCGCGTCGTCGTGGGCTTCGCGCACCACTTTGGGGTCGCGGTTCGAGGTGGTGGGGTCTGAg                                                                                 >  4:370973/1‑149 (MQ=255)
                                                                 gcgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAg                                                                                 >  2:103715/1‑149 (MQ=255)
                                                                    cTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTg                                                                              <  5:243480/149‑1 (MQ=255)
                                                                       ccGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGaa                                                                            <  8:319695/148‑1 (MQ=255)
                                                                       ccGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGaa                                                                            <  5:13744/148‑1 (MQ=255)
                                                                            aCTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGtcgat                                                                      >  8:187654/1‑149 (MQ=255)
                                                                             ctcgctcgCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGtcgatc                                                                     <  1:103715/149‑1 (MQ=255)
                                                                                    gcgcAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGc                                                              <  6:171728/149‑1 (MQ=255)
                                                                                    gcgcAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGc                                                              <  1:129315/149‑1 (MQ=255)
                                                                                      gcAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGcc                                                             >  3:339759/1‑148 (MQ=255)
                                                                                        aGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCa                                                            >  4:87967/1‑147 (MQ=255)
                                                                                        aGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCa                                                            >  2:640/1‑147 (MQ=255)
                                                                                         gggTATTGAAGGCCGCCTCGAGTTGTCCGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAg                                                         <  5:294784/149‑1 (MQ=255)
                                                                                         gggTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAg                                                         <  8:110061/149‑1 (MQ=255)
                                                                                         gggTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAg                                                         <  4:139663/149‑1 (MQ=255)
                                                                                            tATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCATGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTAGATCGGACGGCCAAAGgca                                                      >  5:370955/1‑149 (MQ=255)
                                                                                              ttGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacg                                                    >  1:181158/1‑149 (MQ=255)
                                                                                                  aGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgcac                                                 >  4:256813/1‑148 (MQ=255)
                                                                                                  aGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgcac                                                 >  1:235780/1‑148 (MQ=255)
                                                                                                    gccgccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgat                                              <  7:142355/149‑1 (MQ=255)
                                                                                                      cgccTGGAGTTGTACGCCGCGGATGGCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTTGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgatga                                            <  3:238274/149‑1 (MQ=255)
                                                                                                       gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgatgat                                           <  3:256813/149‑1 (MQ=255)
                                                                                                        ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGatgatg                                          <  2:235780/149‑1 (MQ=255)
                                                                                                         cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGt                                         <  8:77007/149‑1 (MQ=255)
                                                                                                               ttGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtgg                                   <  8:29604/149‑1 (MQ=255)
                                                                                                                      ccgcGGAGGTCACCGTTGCTGGCGACGAAGCGTGCAGCGTCGGCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGAGCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGccac                            <  6:295438/149‑1 (MQ=255)
                                                                                                                          ggATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAgg                        <  8:195390/149‑1 (MQ=255)
                                                                                                                               tCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCGTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGGCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCt                    <  6:103096/148‑1 (MQ=255)
                                                                                                                                        cTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGc          <  2:181158/149‑1 (MQ=255)
                                                                                                                                         ccacgacgaCGCTGCAAGCTTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGc          >  5:193182/4‑148 (MQ=255)
                                                                                                                                          cacgacgaCGCTGCAAGCTTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                        <  1:316890/51‑1 (MQ=17)
                                                                                                                                          cacgacgaCGCTGCAAGCTTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                        >  2:316890/3‑53 (MQ=17)
                                                                                                                                            cgacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCGGGTGGCCACCAGGGTCTGTGCCTGGGCCAgg      <  1:640/149‑1 (MQ=255)
                                                                                                                                               cgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCg   <  7:187654/149‑1 (MQ=255)
                                                                                                                                                gaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTAGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCgg  >  2:178169/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGG  >  NC_002947/160367‑160659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑