Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,299,350	G→C	7.3%	A276A (GCC→GCG)	PP_1134 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,299,350	0	G	C	7.3%	250.8 / 4.7	78	A276A (GCC→GCG)	PP_1134	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base C (3/3);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGGTAAACCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGC  >  NC_002947/1299208‑1299495                                                                                                                                               |                                                                                                                                                    cGTGGTAAACCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCCGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTgc                                                                                                                                               <  5:120505/149‑1 (MQ=255)     gTAAACCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCATTGGCGTgcagca                                                                                                                                           >  3:223345/1‑149 (MQ=255)        aaCCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGc                                                                                                                                        >  1:208225/1‑149 (MQ=255)        aaCCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGc                                                                                                                                        >  1:340616/1‑149 (MQ=255)          ccAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCg                                                                                                                                       >  1:13627/1‑148 (MQ=255)            aGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCgg                                                                                                                                      >  3:225977/1‑147 (MQ=255)            aGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGaa                                                                                                                                    <  2:181620/149‑1 (MQ=255)             gCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑g                                                                                                                                  <  7:199327/149‑1 (MQ=255)                 gTAGTCGGCGGCACCAAGTTTTGGGTGGCCGGCATACTTGACCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCTATACCGAAGCCCACCGATAACGTTTTGGCTTCCATGTAGACACCTTAGGCGTGCAGCATGCGGAA‑Ggt                                                                                                                                <  2:48504/147‑1 (MQ=255)                    gTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gc                                                                                                                          <  3:321693/149‑1 (MQ=255)                    gTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gc                                                                                                                          >  8:48924/1‑149 (MQ=255)                     tCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gcg                                                                                                                         >  1:313630/1‑149 (MQ=255)                     tCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gcg                                                                                                                         >  7:121214/1‑149 (MQ=255)                       ggcggcACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCgg                                                                                                                        <  2:13627/148‑1 (MQ=255)                       ggcggcACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACCGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTctaca                                                                                                                         <  3:68554/148‑5 (MQ=38)                             aCCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTctac                                                                                                                          <  1:346122/141‑4 (MQ=38)                             aCCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTctac                                                                                                                          >  2:346122/1‑138 (MQ=38)                               ccAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTgg                                                                                                               >  5:84845/1‑149 (MQ=255)                                cAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGc                                                                                                              >  3:310146/1‑149 (MQ=255)                                cAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTc                                                                                                                             <  5:294002/135‑1 (MQ=38)                                cAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTc                                                                                                                             >  6:294002/1‑135 (MQ=38)                                 aGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGcc                                                                                                             >  7:142482/1‑149 (MQ=255)                                      tGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAgg                                                                                                         <  5:4779/148‑1 (MQ=255)                                            gCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATa                                                                                                  >  1:307184/1‑149 (MQ=255)                                            gCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATa                                                                                                  >  2:113172/1‑149 (MQ=255)                                                gCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACca                                                                                               >  1:353488/1‑148 (MQ=255)                                                    aCTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATgg                                                                                          >  4:56412/1‑149 (MQ=255)                                                     cTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATgg                                                                                          <  3:56412/148‑1 (MQ=255)                                                     cTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGgc                                                                                         <  6:84845/149‑1 (MQ=255)                                                      ttGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGgcg                                                                                        >  4:36482/1‑149 (MQ=255)                                                         cccAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGa                                                                                     <  5:252303/149‑1 (MQ=255)                                                           cAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGt                                                                                   <  3:36482/149‑1 (MQ=255)                                                           cAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGt                                                                                   <  8:121214/149‑1 (MQ=255)                                                            aGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTg                                                                                  <  6:74212/149‑1 (MQ=255)                                                            aGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTg                                                                                  <  8:142482/149‑1 (MQ=255)                                                              gCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAACGGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGc                                                                                 <  1:91368/149‑1 (MQ=255)                                                              gCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGc                                                                                 >  4:364245/1‑148 (MQ=255)                                                               cGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCaa                                                                               <  3:364245/149‑1 (MQ=255)                                                               cGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCaa                                                                               <  7:48924/149‑1 (MQ=255)                                                                gTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAAc                                                                              >  7:68526/1‑149 (MQ=255)                                                                       gTCGATCAGCTGTTGCGGGTGCTCGATTCGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTcg                                                                        <  7:151298/148‑1 (MQ=255)                                                                          gATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACGTGGCGCGAGTGCAACTGCTcgccgc                                                                    <  4:310146/149‑1 (MQ=255)                                                                            tCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCtt                                                                  <  1:266727/149‑1 (MQ=255)                                                                                 gTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGGTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAg                                                             >  8:244166/1‑149 (MQ=255)                                                                                 gTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAg                                                             <  1:113172/149‑1 (MQ=255)                                                                                      cGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATg                                                                                           <  1:340829/114‑1 (MQ=255)                                                                                      cGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATg                                                                                           >  2:340829/1‑114 (MQ=255)                                                                                              cGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGt                                                 >  7:338951/1‑148 (MQ=255)                                                                                               gATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGt                                                 >  4:327178/1‑147 (MQ=255)                                                                                                   cGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTg                                           <  4:225977/149‑1 (MQ=255)                                                                                                    ggAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTgg                                          >  5:168450/1‑149 (MQ=255)                                                                                                     gAAGACCACCGAGAACGGTTTGGCTTCCATGTAGACAACTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTTGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCTGTCAGTTGGc                                         <  6:303559/149‑1 (MQ=255)                                                                                                          ccACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCt                                    <  8:68526/149‑1 (MQ=255)                                                                                                           cACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTCTGCGGGCGCTGTGGCCCTGGGCCacagc                                                                                                 <  1:214197/88‑5 (MQ=255)                                                                                                            aCCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCa                                               >  8:151134/1‑136 (MQ=255)                                                                                                            aCCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCa                                               <  7:151134/136‑1 (MQ=255)                                                                                                            aCCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTc                                  >  5:111512/1‑149 (MQ=255)                                                                                                              cGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAgg                                                                                                         >  8:101446/1‑76 (MQ=255)                                                                                                              cGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGa                                <  6:168450/149‑1 (MQ=255)                                                                                                              cGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGa                                <  3:327178/149‑1 (MQ=255)                                                                                                                agaACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGAt                               >  5:583/1‑148 (MQ=255)                                                                                                                agaACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATc                              <  2:208225/149‑1 (MQ=255)                                                                                                                 gaACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATc                              >  8:339125/1‑148 (MQ=255)                                                                                                                  aaCGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCaa                            <  2:307184/149‑1 (MQ=255)                                                                                                                     ggTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAg                         >  6:205686/1‑149 (MQ=255)                                                                                                                      gTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAgg                        <  3:13503/149‑1 (MQ=255)                                                                                                                           gCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGt                   <  2:340616/149‑1 (MQ=255)                                                                                                                                 aTGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCt              >  3:32427/1‑148 (MQ=255)                                                                                                                                      gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGc                                                                                                       >  5:249246/1‑54 (MQ=255)                                                                                                                                      gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGc                                                                                                       <  6:249246/54‑1 (MQ=255)                                                                                                                                      gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt        <  6:583/149‑1 (MQ=255)                                                                                                                                      gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt        >  3:33119/1‑149 (MQ=255)                                                                                                                                      gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt        <  8:338951/149‑1 (MQ=255)                                                                                                                                      gACACCTTCCGCATGCTGCACGCCAAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt        >  6:108612/1‑149 (MQ=255)                                                                                                                                       acacCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTg       >  2:328964/1‑149 (MQ=255)                                                                                                                                         acCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTgg      >  6:146460/1‑148 (MQ=255)                                                                                                                                         acCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGa     <  4:223345/149‑1 (MQ=255)                                                                                                                                              tGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAgc  <  2:353488/147‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    CGTGGTAAACCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGC  >  NC_002947/1299208‑1299495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑