Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,299,361	T→C	7.7%	M273V (ATG→GTG)	PP_1134 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,299,361	0	T	C	7.7%	220.8 / 5.7	77	M273V (ATG→GTG)	PP_1134	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/38);  new base C (3/3);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCC  >  NC_002947/1299215‑1299508                                                                                                                                                   |                                                                                                                                                      aaCCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGc                                                                                                                                                     >  1:208225/1‑149 (MQ=255) aaCCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGc                                                                                                                                                     >  1:340616/1‑149 (MQ=255)   ccAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCg                                                                                                                                                    >  1:13627/1‑148 (MQ=255)     aGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCgg                                                                                                                                                   >  3:225977/1‑147 (MQ=255)     aGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGaa                                                                                                                                                 <  2:181620/149‑1 (MQ=255)      gCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑g                                                                                                                                               <  7:199327/149‑1 (MQ=255)          gTAGTCGGCGGCACCAAGTTTTGGGTGGCCGGCATACTTGACCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCTATACCGAAGCCCACCGATAACGTTTTGGCTTCCATGTAGACACCTTAGGCGTGCAGCATGCGGAA‑Ggt                                                                                                                                             <  2:48504/147‑1 (MQ=255)             gTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gc                                                                                                                                       >  8:48924/1‑149 (MQ=255)             gTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gc                                                                                                                                       <  3:321693/149‑1 (MQ=255)              tCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gcg                                                                                                                                      >  7:121214/1‑149 (MQ=255)              tCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTc‑gcg                                                                                                                                      >  1:313630/1‑149 (MQ=255)                ggcggcACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCgg                                                                                                                                     <  2:13627/148‑1 (MQ=255)                ggcggcACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACCGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTctaca                                                                                                                                      <  3:68554/148‑5 (MQ=38)                      aCCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTctac                                                                                                                                       <  1:346122/141‑4 (MQ=38)                      aCCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTctac                                                                                                                                       >  2:346122/1‑138 (MQ=38)                        ccAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTgg                                                                                                                            >  5:84845/1‑149 (MQ=255)                         cAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGc                                                                                                                           >  3:310146/1‑149 (MQ=255)                         cAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTc                                                                                                                                          <  5:294002/135‑1 (MQ=38)                         cAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTCCGCATGCTGCACGCCAAA‑GGTGTc                                                                                                                                          >  6:294002/1‑135 (MQ=38)                          aGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGcc                                                                                                                          >  7:142482/1‑149 (MQ=255)                               tGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAgg                                                                                                                      <  5:4779/148‑1 (MQ=255)                                     gCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATa                                                                                                               >  2:113172/1‑149 (MQ=255)                                     gCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATa                                                                                                               >  1:307184/1‑149 (MQ=255)                                         gCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACca                                                                                                            >  1:353488/1‑148 (MQ=255)                                             aCTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATgg                                                                                                       >  4:56412/1‑149 (MQ=255)                                              cTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATgg                                                                                                       <  3:56412/148‑1 (MQ=255)                                              cTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGgc                                                                                                      <  6:84845/149‑1 (MQ=255)                                               ttGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGgcg                                                                                                     >  4:36482/1‑149 (MQ=255)                                                  cccAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGa                                                                                                  <  5:252303/149‑1 (MQ=255)                                                    cAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGt                                                                                                <  3:36482/149‑1 (MQ=255)                                                    cAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGt                                                                                                <  8:121214/149‑1 (MQ=255)                                                     aGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTg                                                                                               <  8:142482/149‑1 (MQ=255)                                                     aGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTg                                                                                               <  6:74212/149‑1 (MQ=255)                                                       gCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAACGGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGc                                                                                              <  1:91368/149‑1 (MQ=255)                                                       gCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGc                                                                                              >  4:364245/1‑148 (MQ=255)                                                        cGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCaa                                                                                            <  3:364245/149‑1 (MQ=255)                                                        cGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCaa                                                                                            <  7:48924/149‑1 (MQ=255)                                                         gTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAAc                                                                                           >  7:68526/1‑149 (MQ=255)                                                                gTCGATCAGCTGTTGCGGGTGCTCGATTCGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTcg                                                                                     <  7:151298/148‑1 (MQ=255)                                                                   gATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACGTGGCGCGAGTGCAACTGCTcgccgc                                                                                 <  4:310146/149‑1 (MQ=255)                                                                     tCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCtt                                                                               <  1:266727/149‑1 (MQ=255)                                                                          gTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGGTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAg                                                                          >  8:244166/1‑149 (MQ=255)                                                                          gTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAg                                                                          <  1:113172/149‑1 (MQ=255)                                                                               cGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATg                                                                                                        <  1:340829/114‑1 (MQ=255)                                                                               cGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATg                                                                                                        >  2:340829/1‑114 (MQ=255)                                                                                       cGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGt                                                              >  7:338951/1‑148 (MQ=255)                                                                                        gATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGt                                                              >  4:327178/1‑147 (MQ=255)                                                                                            cGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTg                                                        <  4:225977/149‑1 (MQ=255)                                                                                             ggAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTgg                                                       >  5:168450/1‑149 (MQ=255)                                                                                              gAAGACCACCGAGAACGGTTTGGCTTCCATGTAGACAACTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTTGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCTGTCAGTTGGc                                                      <  6:303559/149‑1 (MQ=255)                                                                                                   ccACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCt                                                 <  8:68526/149‑1 (MQ=255)                                                                                                    cACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTCTGCGGGCGCTGTGGCCCTGGGCCacagc                                                                                                              <  1:214197/88‑5 (MQ=255)                                                                                                     aCCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCa                                                            >  8:151134/1‑136 (MQ=255)                                                                                                     aCCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCa                                                            <  7:151134/136‑1 (MQ=255)                                                                                                     aCCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTc                                               >  5:111512/1‑149 (MQ=255)                                                                                                       cGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAgg                                                                                                                      >  8:101446/1‑76 (MQ=255)                                                                                                       cGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGa                                             <  6:168450/149‑1 (MQ=255)                                                                                                       cGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGa                                             <  3:327178/149‑1 (MQ=255)                                                                                                         agaACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGAt                                            >  5:583/1‑148 (MQ=255)                                                                                                         agaACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATc                                           <  2:208225/149‑1 (MQ=255)                                                                                                          gaACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATc                                           >  8:339125/1‑148 (MQ=255)                                                                                                           aaCGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCaa                                         <  2:307184/149‑1 (MQ=255)                                                                                                              ggTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAg                                      >  6:205686/1‑149 (MQ=255)                                                                                                               gTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAgg                                     <  3:13503/149‑1 (MQ=255)                                                                                                                    gCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGt                                <  2:340616/149‑1 (MQ=255)                                                                                                                          aTGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCt                           >  3:32427/1‑148 (MQ=255)                                                                                                                               gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGc                                                                                                                    <  6:249246/54‑1 (MQ=255)                                                                                                                               gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGc                                                                                                                    >  5:249246/1‑54 (MQ=255)                                                                                                                               gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt                     <  8:338951/149‑1 (MQ=255)                                                                                                                               gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt                     >  3:33119/1‑149 (MQ=255)                                                                                                                               gACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt                     <  6:583/149‑1 (MQ=255)                                                                                                                               gACACCTTCCGCATGCTGCACGCCAAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCt                     >  6:108612/1‑149 (MQ=255)                                                                                                                                acacCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTg                    >  2:328964/1‑149 (MQ=255)                                                                                                                                  acCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTgg                   >  6:146460/1‑148 (MQ=255)                                                                                                                                  acCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGa                  <  4:223345/149‑1 (MQ=255)                                                                                                                                       tGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAgc               <  2:353488/147‑1 (MQ=255)                                                                                                                                                gcaTGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGcc    <  4:109366/149‑1 (MQ=255)                                                                                                                                                   tGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGACGCTTTCAAGCACTACACCGGTCAGTTTGCCATCTTCGATAAACAGGTCGGTGACCTTCTCCTGGAAGCTCACTTCGTcccc  >  5:280972/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                      AACCAGCTTGTAGTCGGCGGCACCCAGTTTTGGGTGGCCGGCATACTTGCCCAGGCGTGCCTTGTCGATCAGCGTTTGCGGGTGCTCGATACGGAAGCCCACCGAGAACGGTTTGGCTTCCATGTAGACACCTTTGGCGTGCAGCATGCGGAA‑GGTGTC‑GCGGGCGCTGTGGCCCAGGGCCAATACCACATGGCGCGAGTGCAACTGCTCGCCGCTTTCAAGCACTACACCGGTCAGTTGGCCATCTTCGATCAACAGGTCGGTGACCTTCTCCTGGAAGCGCACTTCGGCCCC  >  NC_002947/1299215‑1299508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑