Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,356,030	A→G	6.0%	intergenic (+297/‑95)	PP_1180 → / → PP_1181	hypothetical protein/two‑component system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,356,030	0	A	G	6.0%	201.6 / 2.3	67	intergenic (+297/‑95)	PP_1180/PP_1181	hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base G (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCC  >  NC_002947/1355891‑1356178                                                                                                                                            |                                                                                                                                                      ccAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCcgcg                                                                                                                                              <  5:300954/149‑1 (MQ=255)   aaGTGGTTTGCAGTATTTTTCTGCACCGTGAATCCCTTGGGCAGTTTTTTTTGGGCGCTAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                            <  6:341889/149‑1 (MQ=255)   aaGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                            >  3:323668/1‑149 (MQ=255)    aGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                                           <  6:184064/149‑1 (MQ=255)      tGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTATGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGgcg                                                                                                                                         >  7:151290/1‑149 (MQ=255)        gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                                       >  1:383504/1‑149 (MQ=255)            gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTc                                                                                                                                      >  7:27131/1‑146 (MQ=255)            gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTc                                                                                                                                      <  8:27131/146‑1 (MQ=255)            gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGt                                                                                                                                   >  4:59613/1‑149 (MQ=255)             cAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtc                                                                                                                                  >  7:93131/1‑149 (MQ=255)              aGTATTTTTCTGCACCTGGCAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtct                                                                                                                                 <  3:91666/149‑1 (MQ=255)              aGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtct                                                                                                                                 >  6:325403/1‑149 (MQ=255)               gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                            >  3:203959/1‑137 (MQ=255)               gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                            <  4:203959/137‑1 (MQ=255)               gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGC‑GGGCGCTCGGTCTCg                                                                                                                               <  6:346876/149‑1 (MQ=255)                 aTTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCg                                                                                                                               >  4:25691/1‑148 (MQ=255)                  tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGGCCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                             <  6:368049/149‑1 (MQ=25)                       cTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGc                                                                                                                         <  2:383504/148‑1 (MQ=255)                       cTGCACCGTCAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTCCAGTCGCGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGCGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAggac                                                                                                                         <  5:152811/148‑3 (MQ=255)                          cACCGTGAAGCCCTTGGGCAG‑TTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTATCGCAGGGccccc                                                                                                                     >  3:195258/1‑148 (MQ=255)                                 aaGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCACCTCTa                                                                                                               >  4:257578/1‑148 (MQ=255)                                            cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActct                                                                                                   <  8:93131/149‑1 (MQ=255)                                             aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActct                                                                                                   >  5:134942/1‑148 (MQ=255)                                              gTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCa                                                                                                 <  3:25691/149‑1 (MQ=255)                                               ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                <  5:325403/149‑1 (MQ=255)                                               ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                >  6:189053/1‑149 (MQ=255)                                               ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                >  6:192720/1‑149 (MQ=255)                                                         gcgcGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGac                                                                                     <  3:59613/149‑1 (MQ=255)                                                                     tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAg                                                                          <  6:134942/148‑1 (MQ=255)                                                                     tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAg                                                                          <  5:192720/148‑1 (MQ=255)                                                                        caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                       >  3:76260/1‑148 (MQ=255)                                                                        caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                       >  2:180941/1‑148 (MQ=255)                                                                        caAAAAACGGTTCAGTCGAGGGGGCGGTAGCGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                       >  1:32649/1‑148 (MQ=255)                                                                         aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGCAACGCTCCCAAGaacaa                                                                     >  1:122585/1‑149 (MQ=255)                                                                         aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaa                                                                     >  8:210050/1‑149 (MQ=255)                                                                         aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaa                                                                     >  1:73100/1‑149 (MQ=255)                                                                          aaaaaCGGCTCAGTCGCGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaac                                                                    <  6:335553/149‑1 (MQ=255)                                                                            aaccGGTGCCGTCGAGGGGGCGGTAGGGCGGGGCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCGATCTACATTCATACTGTCAGCTG‑CCCTGACACGATCCCAAGAAcaacaa                                                                  <  4:250943/146‑1 (MQ=255)                                                                                            gggtCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                  <  8:151290/149‑1 (MQ=255)                                                                                                 ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                             >  4:9102/1‑149 (MQ=255)                                                                                                 ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                             <  4:44481/149‑1 (MQ=255)                                                                                                 ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                             >  5:284114/1‑149 (MQ=255)                                                                                                       gCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGCCCAAAGCCATGGACCCCCCCCTAcc                                       >  6:371447/1‑149 (MQ=255)                                                                                                         gtgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcccc                                     <  6:284114/149‑1 (MQ=255)                                                                                                          tgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCg                                    <  1:180941/149‑1 (MQ=255)                                                                                                              tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                >  6:259503/1‑149 (MQ=255)                                                                                                                caGATCGGGGGTGCCTGGGAGATCGAGCACCTCCCGCGGGGCGCTCGGTCTCGCAGGGCCACCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                <  2:122585/146‑1 (MQ=255)                                                                                                                aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                <  4:76260/147‑1 (MQ=255)                                                                                                                aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                              >  6:2129/1‑149 (MQ=255)                                                                                                                  gATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCa                            <  2:32649/149‑1 (MQ=255)                                                                                                                   aTCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAt                           >  1:93443/1‑149 (MQ=255)                                                                                                                    tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATA‑CCCGCATCCTc                             <  6:250464/145‑1 (MQ=255)                                                                                                                    tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATA‑CCCGCATCCTc                             >  5:250464/1‑145 (MQ=255)                                                                                                                        gggTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtc                          <  5:189053/145‑1 (MQ=255)                                                                                                                         gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCa                                  <  3:283114/131‑1 (MQ=18)                                                                                                                         gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCa                                  >  4:283114/5‑135 (MQ=18)                                                                                                                          gTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                    <  2:93443/149‑1 (MQ=255)                                                                                                                            gCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaaca                  >  4:171439/1‑147 (MQ=255)                                                                                                                                gggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac              >  2:18271/1‑149 (MQ=255)                                                                                                                                 ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac              >  1:64833/1‑148 (MQ=255)                                                                                                                                 ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga               <  3:9102/147‑1 (MQ=255)                                                                                                                                  gtgttCGAGGACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACATGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCTTCGTCGAAGACGACCa            <  4:195258/145‑1 (MQ=255)                                                                                                                                  gagaTCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac              >  5:138572/1‑147 (MQ=255)                                                                                                                                   agaCCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAg           >  3:269449/1‑149 (MQ=38)                                                                                                                                         aGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCTCGCATCCTCATCGTCGAAGACGACCAGCGCCTc     >  8:35870/1‑149 (MQ=255)                                                                                                                                           cACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGc   <  5:2129/149‑1 (MQ=255)                                                                                                                                           cACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGc   >  5:341672/1‑149 (MQ=255)                                                                                                                                            aCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCCAGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGAACAACCCATCCCCCGCAACCTCAACGTCGAAGACGCCCACCGccaagc   >  1:111024/1‑145 (MQ=255)                                                                                                                                            aCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGCACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGcc  <  4:323668/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                      CCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCC  >  NC_002947/1355891‑1356178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑