Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,035 C→G 5.8% intergenic (+302/‑90) PP_1180 → / → PP_1181 hypothetical protein/two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,0350CG 5.8% 216.2 / 3.8 69intergenic (+302/‑90)PP_1180/PP_1181hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base G (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGA  >  NC_002947/1355891‑1356180
                                                                                                                                                |                                                                                                                                                  
ccAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCcgcg                                                                                                                                                <  5:300954/149‑1 (MQ=255)
  aaGTGGTTTGCAGTATTTTTCTGCACCGTGAATCCCTTGGGCAGTTTTTTTTGGGCGCTAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                              <  6:341889/149‑1 (MQ=255)
  aaGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                              >  3:323668/1‑149 (MQ=255)
   aGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                                             <  6:184064/149‑1 (MQ=255)
     tGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTATGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGgcg                                                                                                                                           >  7:151290/1‑149 (MQ=255)
       gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                                         >  1:383504/1‑149 (MQ=255)
           gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTc                                                                                                                                        <  8:27131/146‑1 (MQ=255)
           gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTc                                                                                                                                        >  7:27131/1‑146 (MQ=255)
           gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGt                                                                                                                                     >  4:59613/1‑149 (MQ=255)
            cAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtc                                                                                                                                    >  7:93131/1‑149 (MQ=255)
             aGTATTTTTCTGCACCTGGCAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtct                                                                                                                                   <  3:91666/149‑1 (MQ=255)
             aGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtct                                                                                                                                   >  6:325403/1‑149 (MQ=255)
              gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                              <  4:203959/137‑1 (MQ=255)
              gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCggg                                                                                                                                              >  3:203959/1‑137 (MQ=255)
              gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGC‑GGGCGCTCGGTCTCg                                                                                                                                 <  6:346876/149‑1 (MQ=255)
                aTTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCg                                                                                                                                 >  4:25691/1‑148 (MQ=255)
                 tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGGCCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                               <  6:368049/149‑1 (MQ=25)
                      cTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGc                                                                                                                           <  2:383504/148‑1 (MQ=255)
                      cTGCACCGTCAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTCCAGTCGCGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGCGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAggac                                                                                                                           <  5:152811/148‑3 (MQ=255)
                         cACCGTGAAGCCCTTGGGCAG‑TTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTATCGCAGGGccccc                                                                                                                       >  3:195258/1‑148 (MQ=255)
                                aaGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCACCTCTa                                                                                                                 >  4:257578/1‑148 (MQ=255)
                                           cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActct                                                                                                     <  8:93131/149‑1 (MQ=255)
                                            aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActct                                                                                                     >  5:134942/1‑148 (MQ=255)
                                             gTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCa                                                                                                   <  3:25691/149‑1 (MQ=255)
                                              ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                  <  5:325403/149‑1 (MQ=255)
                                              ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                  >  6:189053/1‑149 (MQ=255)
                                              ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                  >  6:192720/1‑149 (MQ=255)
                                                        gcgcGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGac                                                                                       <  3:59613/149‑1 (MQ=255)
                                                                    tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAg                                                                            <  6:134942/148‑1 (MQ=255)
                                                                    tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAg                                                                            <  5:192720/148‑1 (MQ=255)
                                                                       caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                         >  3:76260/1‑148 (MQ=255)
                                                                       caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                         >  2:180941/1‑148 (MQ=255)
                                                                       caAAAAACGGTTCAGTCGAGGGGGCGGTAGCGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                         >  1:32649/1‑148 (MQ=255)
                                                                        aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGCAACGCTCCCAAGaacaa                                                                       >  1:122585/1‑149 (MQ=255)
                                                                        aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaa                                                                       >  1:73100/1‑149 (MQ=255)
                                                                        aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaa                                                                       >  8:210050/1‑149 (MQ=255)
                                                                         aaaaaCGGCTCAGTCGCGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaac                                                                      <  6:335553/149‑1 (MQ=255)
                                                                           aaccGGTGCCGTCGAGGGGGCGGTAGGGCGGGGCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCGATCTACATTCATACTGTCAGCTG‑CCCTGACACGATCCCAAGAAcaacaa                                                                    <  4:250943/146‑1 (MQ=255)
                                                                                           gggtCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                    <  8:151290/149‑1 (MQ=255)
                                                                                                ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                               >  5:284114/1‑149 (MQ=255)
                                                                                                ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                               <  4:44481/149‑1 (MQ=255)
                                                                                                ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                               >  4:9102/1‑149 (MQ=255)
                                                                                                      gCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGCCCAAAGCCATGGACCCCCCCCTAcc                                         >  6:371447/1‑149 (MQ=255)
                                                                                                        gtgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcccc                                       <  6:284114/149‑1 (MQ=255)
                                                                                                         tgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCg                                      <  1:180941/149‑1 (MQ=255)
                                                                                                             tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                  >  6:259503/1‑149 (MQ=255)
                                                                                                               caGATCGGGGGTGCCTGGGAGATCGAGCACCTCCCGCGGGGCGCTCGGTCTCGCAGGGCCACCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                  <  2:122585/146‑1 (MQ=255)
                                                                                                               aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                  <  4:76260/147‑1 (MQ=255)
                                                                                                               aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                                >  6:2129/1‑149 (MQ=255)
                                                                                                                 gATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCa                              <  2:32649/149‑1 (MQ=255)
                                                                                                                  aTCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAt                             >  1:93443/1‑149 (MQ=255)
                                                                                                                   tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATA‑CCCGCATCCTc                               >  5:250464/1‑145 (MQ=255)
                                                                                                                   tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATA‑CCCGCATCCTc                               <  6:250464/145‑1 (MQ=255)
                                                                                                                       gggTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtc                            <  5:189053/145‑1 (MQ=255)
                                                                                                                        gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCa                                    <  3:283114/131‑1 (MQ=18)
                                                                                                                        gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCa                                    >  4:283114/5‑135 (MQ=18)
                                                                                                                         gTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                      <  2:93443/149‑1 (MQ=255)
                                                                                                                           gCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaaca                    >  4:171439/1‑147 (MQ=255)
                                                                                                                               gggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                >  2:18271/1‑149 (MQ=255)
                                                                                                                                ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                >  1:64833/1‑148 (MQ=255)
                                                                                                                                ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga                 <  3:9102/147‑1 (MQ=255)
                                                                                                                                 gtgttCGAGGACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACATGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCTTCGTCGAAGACGACCa              <  4:195258/145‑1 (MQ=255)
                                                                                                                                 gagaTCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                >  5:138572/1‑147 (MQ=255)
                                                                                                                                  agaCCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAg             >  3:269449/1‑149 (MQ=38)
                                                                                                                                        aGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCTCGCATCCTCATCGTCGAAGACGACCAGCGCCTc       >  8:35870/1‑149 (MQ=255)
                                                                                                                                          cACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGc     >  5:341672/1‑149 (MQ=255)
                                                                                                                                          cACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGc     <  5:2129/149‑1 (MQ=255)
                                                                                                                                           aCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCCAGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGAACAACCCATCCCCCGCAACCTCAACGTCGAAGACGCCCACCGccaagc     >  1:111024/1‑145 (MQ=255)
                                                                                                                                           aCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGCACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGcc    <  4:323668/149‑1 (MQ=255)
                                                                                                                                            ccgcccgcGGGGCGCTCGGGCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCg   <  4:247185/149‑1 (MQ=255)
                                                                                                                                             cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCCAGAACAACAAGGCCAAGGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGa  >  5:233204/1‑149 (MQ=255)
                                                                                                                                             cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGaccacc                                                <  1:128337/103‑1 (MQ=255)
                                                                                                                                             cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGaccacc                                                >  2:128337/1‑103 (MQ=255)
                                                                                                                                             cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTAGCCg   >  6:223067/1‑148 (MQ=255)
                                                                                                                                              gcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtc                         >  4:115938/1‑125 (MQ=255)
                                                                                                                                              gcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtc                         <  3:115938/125‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGA  >  NC_002947/1355891‑1356180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑