Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,356,054	G→C	6.0%	intergenic (+321/‑71)	PP_1180 → / → PP_1181	hypothetical protein/two‑component system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,356,054	0	G	C	6.0%	214.8 / 4.1	67	intergenic (+321/‑71)	PP_1180/PP_1181	hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base C (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

GTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGAATACCTGCAG  >  NC_002947/1355905‑1356202                                                                                                                                                      |                                                                                                                                                      gTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGC‑GGGCGCTCGGTCTCg                                                                                                                                                       <  6:346876/149‑1 (MQ=255)   aTTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCg                                                                                                                                                       >  4:25691/1‑148 (MQ=255)    tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGGCCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                                                     <  6:368049/149‑1 (MQ=25)         cTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGc                                                                                                                                                 <  2:383504/148‑1 (MQ=255)         cTGCACCGTCAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTCCAGTCGCGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGCGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAggac                                                                                                                                                 <  5:152811/148‑3 (MQ=255)            cACCGTGAAGCCCTTGGGCAG‑TTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTATCGCAGGGccccc                                                                                                                                             >  3:195258/1‑148 (MQ=255)                   aaGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCACCTCTa                                                                                                                                       >  4:257578/1‑148 (MQ=255)                              cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActct                                                                                                                           <  8:93131/149‑1 (MQ=255)                               aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActct                                                                                                                           >  5:134942/1‑148 (MQ=255)                                gTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCa                                                                                                                         <  3:25691/149‑1 (MQ=255)                                 ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                                        <  5:325403/149‑1 (MQ=255)                                 ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                                        >  6:189053/1‑149 (MQ=255)                                 ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                                        >  6:192720/1‑149 (MQ=255)                                           gcgcGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGac                                                                                                             <  3:59613/149‑1 (MQ=255)                                                       tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAg                                                                                                  <  5:192720/148‑1 (MQ=255)                                                       tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAg                                                                                                  <  6:134942/148‑1 (MQ=255)                                                          caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                                               >  2:180941/1‑148 (MQ=255)                                                          caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                                               >  3:76260/1‑148 (MQ=255)                                                          caAAAAACGGTTCAGTCGAGGGGGCGGTAGCGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaac                                                                                               >  1:32649/1‑148 (MQ=255)                                                           aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGCAACGCTCCCAAGaacaa                                                                                             >  1:122585/1‑149 (MQ=255)                                                           aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaa                                                                                             >  1:73100/1‑149 (MQ=255)                                                           aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaa                                                                                             >  8:210050/1‑149 (MQ=255)                                                            aaaaaCGGCTCAGTCGCGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGaacaac                                                                                            <  6:335553/149‑1 (MQ=255)                                                              aaccGGTGCCGTCGAGGGGGCGGTAGGGCGGGGCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCGATCTACATTCATACTGTCAGCTG‑CCCTGACACGATCCCAAGAAcaacaa                                                                                          <  4:250943/146‑1 (MQ=255)                                                                              gggtCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGac                                                                          <  8:151290/149‑1 (MQ=255)                                                                                   ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                                                     <  4:44481/149‑1 (MQ=255)                                                                                   ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                                                     >  4:9102/1‑149 (MQ=255)                                                                                   ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                                                     >  5:284114/1‑149 (MQ=255)                                                                                         gCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGCCCAAAGCCATGGACCCCCCCCTAcc                                                               >  6:371447/1‑149 (MQ=255)                                                                                           gtgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcccc                                                             <  6:284114/149‑1 (MQ=255)                                                                                            tgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCg                                                            <  1:180941/149‑1 (MQ=255)                                                                                                tGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                                        >  6:259503/1‑149 (MQ=255)                                                                                                  caGATCGGGGGTGCCTGGGAGATCGAGCACCTCCCGCGGGGCGCTCGGTCTCGCAGGGCCACCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                                        <  2:122585/146‑1 (MQ=255)                                                                                                  aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                                                        <  4:76260/147‑1 (MQ=255)                                                                                                  aaGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCt                                                      >  6:2129/1‑149 (MQ=255)                                                                                                    gATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCa                                                    <  2:32649/149‑1 (MQ=255)                                                                                                     aTCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAt                                                   >  1:93443/1‑149 (MQ=255)                                                                                                      tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATA‑CCCGCATCCTc                                                     <  6:250464/145‑1 (MQ=255)                                                                                                      tCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATA‑CCCGCATCCTc                                                     >  5:250464/1‑145 (MQ=255)                                                                                                          gggTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtc                                                  <  5:189053/145‑1 (MQ=255)                                                                                                           gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCa                                                          >  4:283114/5‑135 (MQ=18)                                                                                                           gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCa                                                          <  3:283114/131‑1 (MQ=18)                                                                                                            gTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                                            <  2:93443/149‑1 (MQ=255)                                                                                                              gCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaaca                                          >  4:171439/1‑147 (MQ=255)                                                                                                                  gggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                                      >  2:18271/1‑149 (MQ=255)                                                                                                                   ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                                      >  1:64833/1‑148 (MQ=255)                                                                                                                   ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga                                       <  3:9102/147‑1 (MQ=255)                                                                                                                    gtgttCGAGGACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACATGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCTTCGTCGAAGACGACCa                                    <  4:195258/145‑1 (MQ=255)                                                                                                                    gagaTCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac                                      >  5:138572/1‑147 (MQ=255)                                                                                                                     agaCCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAg                                   >  3:269449/1‑149 (MQ=38)                                                                                                                           aGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCTCGCATCCTCATCGTCGAAGACGACCAGCGCCTc                             >  8:35870/1‑149 (MQ=255)                                                                                                                             cACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGc                           <  5:2129/149‑1 (MQ=255)                                                                                                                             cACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGc                           >  5:341672/1‑149 (MQ=255)                                                                                                                              aCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCCAGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGAACAACCCATCCCCCGCAACCTCAACGTCGAAGACGCCCACCGccaagc                           >  1:111024/1‑145 (MQ=255)                                                                                                                              aCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGCACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGcc                          <  4:323668/149‑1 (MQ=255)                                                                                                                               ccgcccgcGGGGCGCTCGGGCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCg                         <  4:247185/149‑1 (MQ=255)                                                                                                                                cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCCAGAACAACAAGGCCAAGGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGa                        >  5:233204/1‑149 (MQ=255)                                                                                                                                cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGaccacc                                                                      <  1:128337/103‑1 (MQ=255)                                                                                                                                cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGaccacc                                                                      >  2:128337/1‑103 (MQ=255)                                                                                                                                cgcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTAGCCg                         >  6:223067/1‑148 (MQ=255)                                                                                                                                 gcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtc                                               >  4:115938/1‑125 (MQ=255)                                                                                                                                 gcccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtc                                               <  3:115938/125‑1 (MQ=255)                                                                                                                                    cgcgGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACt                     >  5:179774/1‑148 (MQ=255)                                                                                                                                     gcgGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCTCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCa                   <  7:35870/149‑1 (MQ=255)                                                                                                                                            gcTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCAccgccg             >  4:322912/1‑148 (MQ=255)                                                                                                                                             cTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGaa           <  2:73100/149‑1 (MQ=255)                                                                                                                                             cTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGaa           <  7:210050/149‑1 (MQ=255)                                                                                                                                                ggTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACCCGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGCCCAGCGCCTCGCCGAACTCCCCGCCGAATAc        >  6:58372/1‑149 (MQ=255)                                                                                                                                                  tctcGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGAATACCt      <  2:64833/149‑1 (MQ=255)                                                                                                                                                      gCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGCCACGATCCCAAGAACAACAAGGCCAAAGCCAGGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGCACTCACCGCCGAATACCTGCAg  >  3:55385/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTG‑CCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAACTCACCGCCGAATACCTGCAG  >  NC_002947/1355905‑1356202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑