| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,392,936 | T→C | 17.4% | intergenic (+106/‑80) | PP_1214 → / → ruvC | transcriptional regulator/crossover junction endodeoxyribonuclease RuvC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,392,936 | 0 | T | C | 17.4% | 47.9 / 4.5 | 23 | intergenic (+106/‑80) | PP_1214/ruvC | transcriptional regulator/crossover junction endodeoxyribonuclease RuvC |
| Reads supporting (aligned to +/- strand): ref base T (12/7); new base C (2/2); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01 | |||||||||||
CAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGC > NC_002947/1392788‑1393083 | cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggt > 1:238820/1‑149 (MQ=255)cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGAGGGTAAAAACGCTCATACAGGTATTACGCC‑CATTTAAAATgtggt > 8:61696/1‑149 (MQ=255) aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggtg < 2:238820/149‑1 (MQ=255) aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggtg < 2:361782/149‑1 (MQ=255) aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggt > 1:331183/1‑148 (MQ=255) gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTAc > 8:95261/1‑147 (MQ=255) gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTAc < 7:95261/147‑1 (MQ=255) gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCt < 6:179338/149‑1 (MQ=255) tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAg > 2:253971/1‑149 (MQ=255) ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAg < 5:251833/148‑1 (MQ=255) ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAg < 2:331183/148‑1 (MQ=255) cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGa < 1:55738/149‑1 (MQ=18) acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGc < 7:99775/149‑1 (MQ=18) agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg < 1:253971/149‑1 (MQ=255) tAGCGCAGGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CTTTTCAAATGTGGTCCAGTCCCTGTAGGAGCGGGTTAACCCACGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTa < 2:277803/149‑1 (MQ=37) ccgcgTGTGCCGGCCCTTGCGAGTGTAAACCCTCTCCTACTGGTTTTACGCC‑CATTTAAATTGTGGTGCAGTACCTGTAGGAGCGGTTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTAt < 7:61696/147‑1 (MQ=37) tCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAgg > 7:174910/1‑84 (MQ=255) aaaCCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTTCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACACCgg > 5:31804/1‑149 (MQ=255) aCCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCTCTTTGTAGAGATGCATCATTTTTTCGGCGGGCTTGAAGACTCTGATTCTAGGTATCGACCCCGGCt > 7:247965/1‑149 (MQ=14) ccGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg > 3:82300/1‑149 (MQ=255) ccGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg > 4:80437/1‑149 (MQ=255) cTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCAGCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgcg > 4:272820/1‑148 (MQ=255) cTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgcg > 7:157005/1‑148 (MQ=18) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAccc > 6:184443/1‑123 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCg > 8:48915/1‑149 (MQ=255) gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCg > 4:84328/1‑149 (MQ=255) c‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACACCGGATCGCGCATCACCGGCTATGGCGTg > 3:69631/1‑149 (MQ=255) cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg > 6:301789/1‑149 (MQ=255) gtgCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGc < 5:301789/149‑1 (MQ=255) | CAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGC > NC_002947/1392788‑1393083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |