Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,413,434 T→A 7.5% N155Y (AAC→TAC) ‡ PP_1235 ← AhpC/TSA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,413,4340TA 7.5% 190.4 / 3.5 65N155Y (AAC→TAC) ‡PP_1235AhpC/TSA family protein
Reads supporting (aligned to +/- strand):  ref base T (33/27);  new base A (2/3);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GGGTTTTGGGGGATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGC  >  NC_002947/1413286‑1413579
                                                                                                                                                     |                                                                                                                                                 
gggTTTTGGTGGATATTCTTCGCCATCCCGCTGGCGACGCTGATGAAGGCCGTGCTGGTTGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGtt                                                                                                                                                   <  6:196177/149‑1 (MQ=255)
  gTTTGGGTGGATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCa                                                                                                                                                 <  3:314528/149‑1 (MQ=255)
        ggggATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGAACAAGGCt                                                                                                                                            <  1:232941/148‑1 (MQ=255)
         gggATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTg                                                                                                                                          >  2:25509/1‑149 (MQ=255)
          ggATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTTCTGGATGCGTGGCCCAAGCAGGAGCATA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTgg                                                                                                                                         <  7:168355/149‑1 (MQ=255)
          ggATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTgg                                                                                                                                         <  1:10121/149‑1 (MQ=255)
          ggATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGAACAAGGCTTga                                                                                                                                         <  5:306923/149‑2 (MQ=255)
                tcttcGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTg                                                                                                                                          <  7:245559/142‑1 (MQ=255)
                tcttcGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTg                                                                                                                                          >  8:245559/1‑142 (MQ=255)
                 cttcGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGgccg                                                                                                                                  >  1:247335/1‑149 (MQ=255)
                  ttcGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTAGGTGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGgccg                                                                                                                                  >  1:310511/1‑149 (MQ=255)
                    cGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCCGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGgccgc                                                                                                                                 <  3:219846/147‑1 (MQ=255)
                     gCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCaa                                                                                                                              <  1:292543/149‑1 (MQ=255)
                     gCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCCTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCaa                                                                                                                              <  5:327658/149‑1 (MQ=255)
                               tGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATcc                                                                                                                    <  2:247335/149‑1 (MQ=255)
                                   gACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACAGCATCCACAt                                                                                                                >  1:381881/1‑149 (MQ=255)
                                      gCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGcc                                                                                                             >  3:164403/1‑149 (MQ=255)
                                       cTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGccc                                                                                                            >  8:303015/1‑149 (MQ=255)
                                        tGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCg                                                                                                           >  1:261420/1‑149 (MQ=255)
                                          aTCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCggg                                                                                                         >  7:2834/1‑149 (MQ=255)
                                             aaGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCAc                                                                                                      >  4:253560/1‑149 (MQ=255)
                                              aGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACt                                                                                                     >  2:288670/1‑149 (MQ=255)
                                                     gCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTcacgc                                                                                              >  8:332709/1‑149 (MQ=255)
                                                         gATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGTATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTcacgccacg                                                                                          <  3:253560/149‑1 (MQ=255)
                                                         gATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTcacgccacg                                                                                          >  2:362206/1‑149 (MQ=255)
                                                                 gCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCt                                                                                  >  1:169527/1‑149 (MQ=255)
                                                                  cccAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTg                                                                                 <  7:332709/149‑1 (MQ=255)
                                                                    cAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGgc                                                                               >  7:126995/1‑149 (MQ=255)
                                                                       gCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCgca                                                                            <  1:288670/149‑1 (MQ=255)
                                                                         aGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCTTTCCTGGCgcag                                                                           >  5:74053/1‑148 (MQ=255)
                                                                            aGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCAcc                                                                       <  8:146755/149‑1 (MQ=255)
                                                                            aGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCAcc                                                                       <  7:303015/149‑1 (MQ=255)
                                                                             gCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCACGCCTTGTTCAAGGCTTGGGCGGCAGCCAATACGGCATCCACATGCCCGGGCACTTTCACGACACGCCCTTCATCGCGCAGCAccc                                                                      >  4:10643/1‑149 (MQ=255)
                                                                                tA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCAAATACGGCATCCACATGCCCGGGCACTTTCACGCCCCGCCATTCCTGGACCATCACACCGt                                                                   >  8:102354/1‑149 (MQ=255)
                                                                                   gCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCTTTCCTGGCGCAGCACCCCGTcc                                                                 <  6:74053/149‑1 (MQ=255)
                                                                                     gTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCtt                                                               <  1:362206/149‑1 (MQ=255)
                                                                                     gTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCtt                                                               <  1:25509/149‑1 (MQ=255)
                                                                                          aCCGATGCTTTGAACGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGACACGCCCTTCCTGGCGCAGCACCCCGTCCTAGTTGa                                                          >  8:226025/1‑149 (MQ=255)
                                                                                           ccGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGAt                                                         >  2:110282/1‑149 (MQ=255)
                                                                                            cGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGAt                                                         >  1:368328/1‑148 (MQ=255)
                                                                                            cGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGAt                                                         >  1:225242/1‑148 (MQ=255)
                                                                                             gATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCa                                                       <  2:261420/149‑1 (MQ=255)
                                                                                                 cTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGaa                                                   <  2:310511/149‑1 (MQ=255)
                                                                                                   ttGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAgg                                                 >  1:360427/1‑149 (MQ=255)
                                                                                                        tGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctg                                            <  2:169527/149‑1 (MQ=255)
                                                                                                        tGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctg                                            >  8:227680/1‑149 (MQ=255)
                                                                                                         ttgaatGGGGCTGCCTTGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCACCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctgc                                           >  1:77861/6‑149 (MQ=255)
                                                                                                         gTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGtgctgc                                           >  5:78780/1‑149 (MQ=255)
                                                                                                            aTTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGt                                        <  4:183314/149‑1 (MQ=255)
                                                                                                                  gCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGc                                  >  6:286042/1‑149 (MQ=255)
                                                                                                                        aGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATg                             >  8:25030/1‑148 (MQ=255)
                                                                                                                         ggCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGt                                                                   <  4:99759/109‑1 (MQ=255)
                                                                                                                         ggCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGt                                                                   >  3:99759/1‑109 (MQ=255)
                                                                                                                              ccccATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCt                      >  7:63096/1‑149 (MQ=255)
                                                                                                                               cccATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCtt                     <  5:286042/149‑1 (MQ=255)
                                                                                                                               cccATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACAGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCtt                     <  2:381881/149‑1 (MQ=255)
                                                                                                                                 cATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGc                   <  4:164403/149‑1 (MQ=255)
                                                                                                                                  aTTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGcc                  <  2:225242/149‑1 (MQ=255)
                                                                                                                                      aaTTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATa               >  7:68547/1‑148 (MQ=255)
                                                                                                                                          ccAAGCCTTGAACAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTc                                       <  2:60128/119‑1 (MQ=255)
                                                                                                                                          ccAAGCCTTGAACAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTc                                       >  1:60128/2‑120 (MQ=255)
                                                                                                                                          ccAAGCCTTGAACAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGct          >  3:252969/2‑149 (MQ=255)
                                                                                                                                            aaGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGcttc        >  3:187070/1‑149 (MQ=255)
                                                                                                                                                 ttGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCAt                              <  7:220609/122‑1 (MQ=255)
                                                                                                                                                 ttGTTCAAGGCTTGGGCGGCCGCCAATACGGCATACACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCAt                              >  8:220609/1‑122 (MQ=255)
                                                                                                                                                   gTTCAAGGCTTGGGCGGCCACCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGc  <  2:77861/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGGTTTTGGGGGATATTCTTCGCCATCCCGCTGGCGACGCTGATCAAGGCCGTGCTGGATGCGTGGCCCAAGCAGGAGCCTA‑GCGTTTCACCGATGCTTTGAATGTAATTGGGGCTGCAAGGCAGCCCCATTCAATTTCAAGCCTTGTTCAAGGCTTGGGCGGCCGCCAATACGGCATCCACATGCCCGGGCACTTTCACGCCACGCCATTCCTGGCGCAGCACCCCGTCCTTGTCGATCAGGAAGGTGCTGCGGTCAACGCCCATGTACTCCTTGCCATACAGCTTCTTCAGC  >  NC_002947/1413286‑1413579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑