Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,418,250	T→A	8.6%	*237K (TAA→AAA) ‡	purC →	phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,418,250	0	T	A	8.6%	151.4 / 6.1	58	*237K (TAA→AAA) ‡	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base A (3/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

GTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTGGCAA  >  NC_002947/1418103‑1418398                                                                                                                                                    |                                                                                                                                                     gTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGt                                                                                                                                                      <  4:361718/148‑1 (MQ=255)   gCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAg                                                                                                                                                   >  5:308490/1‑149 (MQ=255)    cTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGc                                                                                                                                                  <  3:35938/149‑1 (MQ=255)         cgacgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTTACAGCGGCAc                                                                                                                                             <  3:144175/149‑1 (MQ=255)                ttCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACg                                                                                                                                      >  3:84371/1‑149 (MQ=255)                ttCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCCCTGTAAGCGGCACGTTCACg                                                                                                                                      >  1:170442/1‑149 (MQ=255)                   aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCAc                                                                                                                                       >  7:270377/1‑145 (MQ=255)                   aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCAc                                                                                                                                       <  8:270377/145‑1 (MQ=255)                   aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGc                                                                                                                                     >  2:134595/1‑147 (MQ=255)                   aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGc                                                                                                                                     >  6:345163/1‑147 (MQ=255)                   aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCaa                                                                                                                                   <  1:287274/149‑1 (MQ=255)                            ggCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGt                                                                                                                                           >  6:200659/1‑132 (MQ=255)                            ggCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGt                                                                                                                                           <  5:200659/132‑1 (MQ=255)                              cTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATAc                                                                                                                        >  4:313575/1‑149 (MQ=255)                              cTGCCGCCTGGGGGACAAGCAACCCCGCAAGAAGATGCCCAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATAc                                                                                                                        <  4:44582/149‑1 (MQ=255)                                   gccTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCAcgc                                                                                                                   <  5:43416/149‑1 (MQ=255)                                           gACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGAtttttt                                                                                                           >  2:84392/1‑149 (MQ=255)                                            aCAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGAttttttt                                                                                                          <  5:345163/149‑1 (MQ=255)                                            aCAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGAttttttt                                                                                                          <  6:360340/149‑1 (MQ=255)                                                ggAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGaa                                                                                                       >  3:329339/1‑148 (MQ=255)                                                ggAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGa                                                                                                        <  8:76160/147‑1 (MQ=255)                                                ggAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGa                                                                                                        >  7:76160/1‑147 (MQ=255)                                                   aaCCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGaaaaaa                                                                                                   <  2:143979/149‑1 (MQ=255)                                                      ccGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCa                                                                                                                                        <  2:49215/109‑1 (MQ=255)                                                      ccGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCa                                                                                                                                        >  1:49215/1‑109 (MQ=255)                                                       cGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGAt                                                                                               >  5:35554/1‑149 (MQ=255)                                                           agaagaTGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCACTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGc                                                                                           <  6:20766/149‑1 (MQ=255)                                                                  ggACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTa                                                                                     >  5:194138/1‑148 (MQ=255)                                                                      aaGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAg                                                                                 >  4:373408/1‑148 (MQ=255)                                                                       aGGACCGCTTCCGCCCGGGCCGGGGCTACGTCATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTTTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGaa                                                                               <  4:5371/149‑1 (MQ=255)                                                                         gACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGGTGTAAGCGGGACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACg                                                                             >  1:366798/1‑149 (MQ=255)                                                                           gcGCTTCCGCCAGGGACTGGGCGACGTTTTGGAATCATTCGAAGAATTTGACAAGCGCCTGGTCGTTCCTCTTTAAGCGTCACGTTCACGCAAGCGCATTATAACACGCGATTTTTTTGAAAAAAATATTTGCGTTTTAAAAGAACGCt                                                                           <  1:84392/148‑1 (MQ=255)                                                                            cGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTg                                                                          <  5:23409/149‑1 (MQ=255)                                                                                 ccGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTa                                                                       <  8:191382/147‑1 (MQ=255)                                                                                   gCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatgat                                                                   >  1:242395/1‑149 (MQ=255)                                                                                    ccAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatgatg                                                                  >  5:106763/1‑149 (MQ=255)                                                                                       gggCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGaaaaaaa                                                                                                  >  8:76590/1‑114 (MQ=255)                                                                                       gggCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGaaaaaaa                                                                                                  <  7:76590/114‑1 (MQ=255)                                                                                        ggCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTACGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGcgcg                                                              <  1:188928/149‑1 (MQ=255)                                                                                              ggCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatgat                                                                   >  4:347884/1‑138 (MQ=255)                                                                                              ggCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatgat                                                                   <  3:347884/138‑1 (MQ=255)                                                                                                cGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga                                                      <  3:373408/149‑1 (MQ=255)                                                                                                 gACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgag                                                     <  2:242395/149‑1 (MQ=255)                                                                                                   cGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTTATACCACGCTATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgag                                                     <  8:200560/147‑1 (MQ=255)                                                                                                     tGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGAt                                                 <  6:194138/149‑1 (MQ=255)                                                                                                                 aCGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTTAGCGGGCCGGTGCCGCAAGCGCCTGATACCACGCGCTTTTTTTGTAAAAAAGATTTGGGTTTTTAAAGAACGGTGGTTTGGTGCGCGGCCTTGGGGAGATGCCGGggtggtg                                     >  8:197270/1‑148 (MQ=255)                                                                                                                     agaagTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGc                                                                                           <  7:46584/91‑1 (MQ=255)                                                                                                                     agaagTTGCCAAGCGCCTGGGCGTGCCGCGGTAAGCGGCACGTTAACGCAAGCGCCGGGTAACACGCGATTTTTTTTAAAAAAAGAttttc                                                                                           >  8:46584/1‑89 (MQ=255)                                                                                                                          ttGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGAc                            <  6:106763/149‑1 (MQ=255)                                                                                                                               aaGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAtt                       >  3:41887/1‑149 (MQ=255)                                                                                                                                aGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTc                      <  4:329339/149‑1 (MQ=255)                                                                                                                                  cgcCTGGGCGGGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGa                    <  6:308490/149‑1 (MQ=255)                                                                                                                                   gcCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGaa                   <  2:170442/149‑1 (MQ=255)                                                                                                                                       gaacGTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATcc               >  5:362364/4‑149 (MQ=255)                                                                                                                                        ggCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATcc               <  6:35554/148‑1 (MQ=255)                                                                                                                                          cGTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCGGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt            >  7:299241/1‑149 (MQ=255)                                                                                                                                           gTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAAc                                 >  4:205231/1‑127 (MQ=255)                                                                                                                                           gTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAAc                                 <  3:205231/127‑1 (MQ=255)                                                                                                                                           gTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTg           >  6:54322/1‑149 (MQ=255)                                                                                                                                                    tAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTGGCaa  >  5:55966/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTGGCAA  >  NC_002947/1418103‑1418398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑