Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 162,292 G→T 8.6% intergenic (‑326/+48) PP_0153 ← / ← scpC hypothetical protein/propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947162,2920GT 8.6% 244.3 / 10.7 81intergenic (‑326/+48)PP_0153/scpChypothetical protein/propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (40/34);  new base T (3/4);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

TGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCTGGCAGGC  >  NC_002947/162146‑162435
                                                                                                                                                    |                                                                                                                                               
tGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAagag                                                                                                                                               <  3:185065/149‑1 (MQ=255)
    ttttGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGGGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCg                                                                                                                                           <  5:95627/149‑1 (MQ=255)
            cGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAAGCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGc                                                                                                                                    <  2:215889/148‑1 (MQ=255)
            cGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGAATGGGCCATAACAgt                                                                                                                                    <  1:317683/148‑2 (MQ=37)
            cGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGc                                                                                                                                    <  5:348514/148‑1 (MQ=255)
             gaagaaGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCg                                                                                                                                   <  3:89351/148‑1 (MQ=255)
             gaagaaGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCg                                                                                                                                   >  1:66677/1‑148 (MQ=255)
              aagaagGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGAATGGGCCATAACAgtcaa                                                                                                                                 <  5:302893/149‑5 (MQ=25)
              aagaagGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCgg                                                                                                                                  >  5:288850/1‑148 (MQ=255)
                   ggggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGAACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGCTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAg                                                                                                                            >  4:110894/1‑149 (MQ=255)
                     ggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAggg                                                                                                                          >  8:217383/1‑149 (MQ=255)
                       aTTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGAGGGCAAGCCCGCGAAGAGGCCGGaa                                                                                                                                        <  2:83672/133‑1 (MQ=255)
                       aTTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGAGGGCAAGCCCGCGAAGAGGCCGGaa                                                                                                                                        >  1:83672/1‑133 (MQ=255)
                            tCAGTCGGGAAGGTCTGAAAGAACCAGCGCAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGt                                                                                                                   >  3:107769/1‑149 (MQ=255)
                              aGTGGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATTACCACCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGt                                                                                                                   <  1:324920/149‑1 (MQ=255)
                              aGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGAATGGGccataa                                                                                                                                        >  8:107821/1‑122 (MQ=25)
                              aGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGAATGGGccataa                                                                                                                                        <  7:107821/126‑5 (MQ=25)
                              aGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTc                                                                                                                 >  6:77017/1‑149 (MQ=255)
                                tCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCg                                                                                                               >  8:203455/1‑149 (MQ=255)
                                    gAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAcc                                                                                                           <  7:203455/149‑1 (MQ=255)
                                    gAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAc                                                                                                            >  6:162844/1‑148 (MQ=255)
                                        gTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCTAGCCCGCGAAGAGGCCGGGACAGCGGAAGCAGGGCCGCCGTTCCGGAcggcat                                                                                                       >  8:275939/1‑144 (MQ=255)
                                         tCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATc                                                                                                      >  6:265506/1‑149 (MQ=255)
                                            gAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCg                                                                                                    >  2:169396/1‑148 (MQ=255)
                                             aaaGAACCAGCACAGTAGTCCAGAGTTT‑‑GGTGCTAGTGGAGGGGGGGCGTAGTGATAGAAGGGGTTGACTGTTATTGCCCATTCGCGGGCAAGCCCGCTAAGAGGCCGGAACAGCGGAATGAGGGCCGCCGTTCCGTACCACATCCgg                                                                                                   <  4:46683/148‑1 (MQ=255)
                                             aaaGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGTGCCGCCGTTCCGTACCACATCCGGa                                                                                                  >  5:205048/1‑149 (MQ=255)
                                              aaGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGAt                                                                                                 <  5:265506/149‑1 (MQ=255)
                                               aGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATc                                                                                                <  5:162844/149‑1 (MQ=255)
                                                     aGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTgg                                                                                          >  1:258901/1‑149 (MQ=255)
                                                       cacaGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTg                                                                                           >  5:258912/1‑146 (MQ=255)
                                                       cacaGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTg                                                                                           <  6:258912/146‑1 (MQ=255)
                                                       cacaGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCAGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGc                                                                                         >  8:112130/1‑148 (MQ=255)
                                                          agtagtACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCa                                                                                       <  5:206515/147‑1 (MQ=255)
                                                              gtaCAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATgc                                                                                 >  7:211880/1‑149 (MQ=255)
                                                               taCAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATgcg                                                                                >  3:4977/1‑149 (MQ=255)
                                                                    aTTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCgg                                                                           >  7:311354/1‑149 (MQ=255)
                                                                       aT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGtt                                                                         <  8:211880/148‑1 (MQ=255)
                                                                             tgctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTCATATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAATCAGGGCCGCCGTTCCGAACCACATCCGGATCAGATGGCCAGCATGCGCCCGGTttctt                                                                     >  5:368662/1‑148 (MQ=255)
                                                                              gctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttcttc                                                                    <  8:311354/148‑1 (MQ=255)
                                                                               ctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttcttc                                                                    >  6:373759/1‑147 (MQ=255)
                                                                                  gtagAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAg                                                                 <  5:340612/147‑1 (MQ=255)
                                                                                     gagGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCGAGGtttt                                                            >  7:315427/1‑149 (MQ=255)
                                                                                        gggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGt                                                         <  2:66677/149‑1 (MQ=255)
                                                                                             gCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAg                                                    <  3:110894/149‑1 (MQ=255)
                                                                                               gTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCt                                                  >  2:259365/1‑149 (MQ=255)
                                                                                                   tGATAGAATGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAg                                               <  2:82581/148‑1 (MQ=255)
                                                                                                     aTAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGc                                            >  1:314413/1‑149 (MQ=255)
                                                                                                        gAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTc                                         >  1:1096/1‑149 (MQ=255)
                                                                                                        gAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTc                                         <  5:77017/149‑1 (MQ=255)
                                                                                                         aaGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTcg                                        >  8:50764/1‑149 (MQ=255)
                                                                                                         aaGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTc                                         <  8:315427/148‑1 (MQ=255)
                                                                                                                  aCTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTc                                         <  3:217904/139‑1 (MQ=255)
                                                                                                                  aCTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTc                                         >  4:217904/1‑139 (MQ=255)
                                                                                                                  aCTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGAt                                <  5:373759/148‑1 (MQ=255)
                                                                                                                    tGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGAtgtg                             >  1:379570/1‑149 (MQ=255)
                                                                                                                      ttATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCg                           <  7:217383/149‑1 (MQ=255)
                                                                                                                        aTGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCggg                         >  7:360451/1‑149 (MQ=255)
                                                                                                                        aTGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCgg                          <  7:112130/148‑1 (MQ=255)
                                                                                                                        aTGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCgg                          <  2:314413/148‑1 (MQ=255)
                                                                                                                         tGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGTGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCggg                         <  6:205048/148‑1 (MQ=255)
                                                                                                                         tGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCgggg                        <  2:258901/149‑1 (MQ=255)
                                                                                                                         tGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCggg                         <  6:288850/148‑1 (MQ=255)
                                                                                                                         tGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCggg                         <  1:259365/148‑1 (MQ=255)
                                                                                                                         cggcctcttCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCgggg                        >  1:95121/8‑149 (MQ=255)
                                                                                                                           gCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTa                      >  4:108301/1‑149 (MQ=255)
                                                                                                                            cccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCa                                                >  6:274933/1‑122 (MQ=255)
                                                                                                                            cccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCa                                                <  5:274933/122‑1 (MQ=255)
                                                                                                                            cccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTAt                     >  7:184433/1‑149 (MQ=255)
                                                                                                                             ccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATg                    >  5:84462/1‑149 (MQ=255)
                                                                                                                             ccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATg                    <  2:310844/149‑1 (MQ=255)
                                                                                                                             ccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATg                    >  3:209691/1‑149 (MQ=255)
                                                                                                                                 tCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGgccg                >  6:168147/1‑149 (MQ=255)
                                                                                                                                   gcgGGCTTGCCCGCGAATAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTTCCAGCTCAGGGCATCGCGCAGGATGTGCGGGGTATGgacgc               >  7:308193/1‑148 (MQ=255)
                                                                                                                                   gcgGGCTTGCCCGCGAATAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAg                                   >  7:89228/1‑128 (MQ=255)
                                                                                                                                   gcgGGCTTGCCCGCGAATAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAg                                   <  8:89228/128‑1 (MQ=255)
                                                                                                                                    cgGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCa             <  4:107769/149‑1 (MQ=255)
                                                                                                                                       gCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAg                                                    <  2:109224/107‑1 (MQ=255)
                                                                                                                                       gCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAg                                                    >  1:109224/1‑107 (MQ=255)
                                                                                                                                        cAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACg           >  1:136691/1‑147 (MQ=255)
                                                                                                                                         aaGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCTg        >  4:69607/1‑149 (MQ=255)
                                                                                                                                              cgcgAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCTGGCAgg   >  2:273531/1‑149 (MQ=255)
                                                                                                                                               gcgAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTCTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCTGGCAGgc  <  5:19668/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTAT‑‑GCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCTGGCAGGC  >  NC_002947/162146‑162435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑