Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,468,805 T→C 6.8% Q446Q (CAA→CAG)  algK ← alginate biosynthesis protein AlgK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,468,8050TC 6.8% 211.8 / 3.4 74Q446Q (CAA→CAG) algKalginate biosynthesis protein AlgK
Reads supporting (aligned to +/- strand):  ref base T (38/30);  new base C (2/3);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCCGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTGGCCCTC  >  NC_002947/1468660‑1468948
                                                                                                                                                 |                                                                                                                                               
aTGCCGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGc                                                                                                                                              <  8:309398/149‑1 (MQ=255)
 tGCCGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCt                                                                                                                                             <  6:139134/149‑1 (MQ=255)
 tGCCGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCCCCAGGCTGCCGCGTGCACGCTTTTCCTGGCCCAGCAACTGCt                                                                                                                                             >  6:245483/1‑149 (MQ=255)
 tGCCGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGGGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGCCCAGCAACTGCt                                                                                                                                             >  6:240079/1‑149 (MQ=255)
    cGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTggg                                                                                                                                          >  4:118180/1‑149 (MQ=255)
       cTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGCCCAGCAACTGCTGGAcca                                                                                                                                       <  5:240079/149‑2 (MQ=255)
       cTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGCCCAGCAACTGCTGGAcca                                                                                                                                       <  5:245483/149‑2 (MQ=255)
        tttCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTg                                                                                                                                            >  4:93811/1‑143 (MQ=255)
        tttCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTg                                                                                                                                            <  3:93811/143‑1 (MQ=255)
              gAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCTCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTgcg                                                                                                                                <  6:115009/149‑1 (MQ=255)
              gAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTgg                                                                                                                                           <  7:33108/138‑1 (MQ=255)
              gAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTgg                                                                                                                                           >  8:33108/1‑138 (MQ=255)
              gAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTgcg                                                                                                                                >  8:154178/1‑149 (MQ=255)
              gAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCGGGGCCTGGTTgcg                                                                                                                                >  4:232314/1‑149 (MQ=255)
                   gATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGAt                                                                                                                           >  5:217120/1‑149 (MQ=255)
                       gAGGGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAg                                                                                                                        <  1:311538/148‑1 (MQ=255)
                       gAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAg                                                                                                                        <  7:194126/148‑1 (MQ=255)
                            tCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCGTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGgtgt                                                                                                                    >  8:228935/1‑147 (MQ=255)
                             cATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGTGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAg                                                                                                                 <  8:164688/149‑1 (MQ=255)
                              aTAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAg                                                                                                                 <  1:72875/148‑1 (MQ=255)
                                   gAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTg                                                                                                           <  3:87565/149‑1 (MQ=255)
                                         tCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTc                                                                                                        >  3:342384/1‑146 (MQ=255)
                                           aCCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAgct                                                                                                   >  2:44607/1‑149 (MQ=255)
                                           aCCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAgc                                                                                                    <  3:340228/148‑1 (MQ=255)
                                            ccGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTGGCGCCCTGCGCCAGGCTGCCGCGGGGACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATGAGGTGTCAGTTGCTGGTCGAgctg                                                                                                  <  7:252442/149‑1 (MQ=255)
                                                   cttcttTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCtgctgc                                                                                              <  6:217120/146‑1 (MQ=255)
                                                   cttcttTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTgcag                                                                                            >  8:356975/1‑148 (MQ=255)
                                                   cttcttTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTCCTATTCGCGCACTGAGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTCGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTgcag                                                                                            >  5:39517/1‑148 (MQ=255)
                                                    ttcttTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTgcag                                                                                            >  4:322662/1‑147 (MQ=255)
                                                      cttTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGt                                                                                        >  4:230917/1‑149 (MQ=255)
                                                         tttCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGCTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGt                                                                                        <  5:354592/146‑1 (MQ=255)
                                                         tttAGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGgt                                                                                                                      >  6:27169/1‑116 (MQ=255)
                                                           tcgtcgTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAACTGCTGCAGCAGCTCga                                                                                    <  7:154178/148‑2 (MQ=255)
                                                              tcgtcTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGa                                                                                >  1:381653/1‑149 (MQ=255)
                                                                 tcTTGAAGGGCTTCGAGGGCCAGGGTGCTATTAGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCAAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGATGGTCGAGCTGCTGCAGCAGTTCGGCTGATTg                                                                             >  7:188428/1‑149 (MQ=255)
                                                                       aGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCTGTTGTTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGt                                                                       >  6:27155/1‑149 (MQ=255)
                                                                        gggCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAgctgc                                                                                                 <  3:123616/122‑1 (MQ=255)
                                                                        gggCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAgctgc                                                                                                 >  4:123616/1‑122 (MQ=255)
                                                                          gCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCgg                                                                    >  7:160321/1‑149 (MQ=255)
                                                                               gAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCCGTTGTTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTgg                                                               <  5:27155/149‑1 (MQ=255)
                                                                                 gggCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGcc                                                             >  5:287979/1‑149 (MQ=255)
                                                                                    ccAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCGTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTGGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGc                                                          <  7:228935/149‑1 (MQ=255)
                                                                                            cTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTg                                                  >  6:382397/1‑149 (MQ=255)
                                                                                             tATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTgg                                                 >  6:315208/1‑149 (MQ=255)
                                                                                              aTTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTggg                                                >  1:237430/1‑149 (MQ=255)
                                                                                               ttCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTgg                                                 <  6:292755/147‑1 (MQ=255)
                                                                                               ttCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGc                                               >  8:219762/1‑149 (MQ=255)
                                                                                                 cgcgCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCg                                              >  5:358958/1‑148 (MQ=255)
                                                                                                  gcgcCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCtgctgc                                                                                              <  3:271129/99‑1 (MQ=255)
                                                                                                  gcgcCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCtgctgc                                                                                              >  4:271129/1‑99 (MQ=255)
                                                                                                       cTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACAccc                                       <  4:342384/149‑1 (MQ=255)
                                                                                                        tGCGCCAGGCTGCCGCGTGCACGCTTTACCTGGTCCAGCAGATGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACAcc                                        >  8:192337/1‑147 (MQ=255)
                                                                                                             cAGGCTGCCGCGTGCACGCTGTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTTGGCGAACAACCAGCCGt                                 >  2:302147/1‑149 (MQ=255)
                                                                                                                 cTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACAcc                                        >  5:192032/1‑138 (MQ=255)
                                                                                                                 cTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACAcc                                        <  6:192032/138‑1 (MQ=255)
                                                                                                                     cgcgTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGt                         >  1:353429/1‑149 (MQ=255)
                                                                                                                      gcgTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGtt                        <  3:230917/149‑1 (MQ=255)
                                                                                                                       cgTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTg                       <  6:39517/149‑1 (MQ=255)
                                                                                                                       cgTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTg                       >  2:202366/1‑149 (MQ=255)
                                                                                                                           cACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTgcggg                   >  5:31979/1‑149 (MQ=255)
                                                                                                                           cACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTAGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTgcggg                   >  7:297715/1‑149 (MQ=255)
                                                                                                                            aCGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTgcgggc                  >  6:383707/1‑149 (MQ=255)
                                                                                                                            aCGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTgcgggc                  >  5:50473/1‑149 (MQ=255)
                                                                                                                                  ttCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGAt              <  3:118180/147‑1 (MQ=255)
                                                                                                                                    ccTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGcc           <  3:232314/148‑1 (MQ=255)
                                                                                                                                        gTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTgg       <  7:219762/148‑1 (MQ=255)
                                                                                                                                        gTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTgg       <  4:257278/148‑1 (MQ=255)
                                                                                                                                        gTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTgg       >  3:257278/1‑148 (MQ=255)
                                                                                                                                          ccAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTGGccc    <  3:322662/149‑1 (MQ=255)
                                                                                                                                          ccAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGGTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTGGccc    <  7:356975/149‑1 (MQ=255)
                                                                                                                                           cagcagTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGaa                                            <  3:82747/108‑1 (MQ=255)
                                                                                                                                           cagcagTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGaa                                            >  4:82747/1‑108 (MQ=255)
                                                                                                                                           cagcagTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTGGCCCt   >  4:82915/1‑149 (MQ=255)
                                                                                                                                            agcagTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCCTGGcccag  >  5:72946/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATGCCGGCTTTCACGAAGGGATTGAGCGTCATAGCGAGTCCTCACCGTCTACTTCTTTTTCGTCGTCTTGCAGGGCTTCGAGGGCCAGGGTGCTATTCGCGCCCTGCGCCAGGCTGCCGCGTGCACGCTTTTCCTGGTCCAGCAGTTGCTGGGCCTGGTTGCGCTGATCAGGTGTCAGTTGCTGGTCGAGCTGCTGCAGCAGTTCGGCTGATTGCGGCGTCGGGTTGGCCTGCGACAGCTGGGCGAACACCCAGGCGTTGCCCGGTTGCGGGCGGATGCCGTGGCCCTC  >  NC_002947/1468660‑1468948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑