Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 165,929 G→T 10.5% A326A (GCC→GCA)  pntAA ← pyridine nucleotide transhydrogenase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947165,9290GT10.5% 148.1 / 7.5 55A326A (GCC→GCA) pntAApyridine nucleotide transhydrogenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base T (3/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

CTCCTTAGCCGTTTTTGCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGCTTCATCT  >  NC_002947/165781‑166074
                                                                                                                                                       |                                                                                                                                                 
cTCCTTAGCCGTTTTTGCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAg                                                                                                                                                   <  8:358730/149‑1 (MQ=255)
        ccGTTTTTGCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCgg                                                                                                                                            >  7:335505/1‑148 (MQ=255)
         cGTTTTTGCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCa                                                                                                                                          <  2:231824/149‑1 (MQ=255)
          gTTTTTGCGGACGACCTGACCATCACGGCAGATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCAc                                                                                                                                         >  1:35999/1‑149 (MQ=255)
              ttGCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGATGCCTCGGCACtgt                                                                                                                                      <  5:82510/148‑3 (MQ=255)
               tgaggACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCGCTCTCTTCGAGGACCATGTTCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGATGCCTCGGCACt                                                                                                                                        <  1:319867/145‑1 (MQ=255)
                gCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATACTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCAGAGGCATCGGCACTCAcat                                                                                                                                   >  4:9341/1‑147 (MQ=255)
                  ggACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTgg                                                                                                                                 >  1:251947/1‑149 (MQ=255)
                        ccTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCgg                                                                                                                            >  3:162532/1‑148 (MQ=255)
                            aCCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGt                                                                                                                       <  1:257153/149‑1 (MQ=255)
                               aTCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAGCAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGTCATCGGCACTCACCTGGGCCGGCAGGTTgg                                                                                                                    <  8:84905/149‑1 (MQ=255)
                               aTCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTgg                                                                                                                    >  3:287024/1‑149 (MQ=255)
                                tCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCAGCCTAGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTGGCGTGCGTCCAGTGCCGAGGCATCGGCACTCCCCGGGGCCGGCAGGTTGGt                                                                                                                   >  8:119725/1‑149 (MQ=255)
                                     gCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGATGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGc                                                                                                              <  2:363784/149‑1 (MQ=255)
                                     gCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGAGGCTCAGCGCGACATCCTTGTCGAACAGCAGCTTCAGGAAGGCCAGCAGGGTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGCCGGGc                                                                                                              <  6:202322/149‑1 (MQ=255)
                                      cacaTCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGcc                                                                                                             <  2:13703/149‑1 (MQ=255)
                                        caTCAGGCAGGCCGGGTCGATGTCG‑‑‑TCTTCGTGGTTGATGATCAGCTCGCGATCCTTGTCGAACATCAGCTTCATGAAGTCGAGCAGGTTGCGTGCTTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCcga                                                                                                           <  4:287024/149‑1 (MQ=255)
                                         aTCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCCTCGGCCCTCCCCTGGGCGGGCAGGGTGGTGGGGCcgag                                                                                                          >  2:299439/1‑148 (MQ=255)
                                         aTCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGAGGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCcgac                                                                                                          <  7:347360/149‑1 (MQ=255)
                                               caggcCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCCAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACgatgat                                                                                                    <  1:316689/149‑1 (MQ=255)
                                                aggcCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACgatgat                                                                                                    >  8:21782/1‑148 (MQ=255)
                                                    cgcgACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGc                                                                                               >  3:358878/1‑149 (MQ=255)
                                                                               gaagatCAGCGAGCCATCCTAGACGAACAGCCGCTTCAAGAAGTCCAGCAGGTTGCCTTCGTACAGTGCCGAGGCAAATCGAATCACCTCGGCCGGCAGGTTGGTCGGGCCGACGATGATGACGGAGTTCTCCTGAACCACGTGGTCTg                                                                       >  4:157785/4‑149 (MQ=255)
                                                                                atgatCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTg                                                                       <  4:358878/148‑1 (MQ=255)
                                                                                    tCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACg                                                                  <  4:162532/149‑1 (MQ=255)
                                                                                            ccATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCgg                                                          <  1:321271/149‑1 (MQ=255)
                                                                                             cATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCggg                                                         >  3:346096/1‑149 (MQ=255)
                                                                                              aTCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGc                                                        <  1:299439/149‑1 (MQ=255)
                                                                                               tCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCggg                                                         >  6:218583/1‑147 (MQ=255)
                                                                                                 cTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAg                                                             <  5:205726/141‑1 (MQ=255)
                                                                                                 cTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAg                                                             >  6:205726/1‑141 (MQ=255)
                                                                                                     tCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTgcc                                                 >  4:167869/1‑149 (MQ=255)
                                                                                                        aaCAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTgccgcc                                              >  1:271991/1‑149 (MQ=255)
                                                                                                            gcagcTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTgg                                          <  2:251947/149‑1 (MQ=255)
                                                                                                                   cATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCa                                    <  4:346096/148‑1 (MQ=255)
                                                                                                                   cATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCa                                    <  3:167869/148‑1 (MQ=255)
                                                                                                                    aTGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGc                                  >  4:46475/1‑149 (MQ=255)
                                                                                                                     tGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCtt                                            <  8:191160/138‑1 (MQ=255)
                                                                                                                     tGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCtt                                            >  7:191160/1‑138 (MQ=255)
                                                                                                                              gcagGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGAt                         >  5:230743/1‑148 (MQ=255)
                                                                                                                                     gcgtgcgtACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGa                       <  5:217406/143‑1 (MQ=255)
                                                                                                                                     gcgtgcgtACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGa                       >  6:217406/1‑143 (MQ=255)
                                                                                                                                           gtACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAg                               >  2:166808/1‑129 (MQ=255)
                                                                                                                                           gtACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAg                               <  1:166808/129‑1 (MQ=255)
                                                                                                                                            tACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCggg          <  7:119725/149‑1 (MQ=255)
                                                                                                                                            tACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCggg          >  1:270431/1‑149 (MQ=255)
                                                                                                                                            gtgaGTGCCGATGCCTCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCggg          >  2:17088/4‑149 (MQ=255)
                                                                                                                                              gaGTGCCGATGCCTCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGCt        >  5:284872/2‑149 (MQ=255)
                                                                                                                                              cAGTTCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGc         <  3:9341/148‑1 (MQ=255)
                                                                                                                                              cAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGc         <  6:230743/148‑1 (MQ=255)
                                                                                                                                               aGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGCtt       >  6:3043/1‑149 (MQ=255)
                                                                                                                                                gTGCCGATGCCTCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGCTTc      >  7:346122/1‑149 (MQ=255)
                                                                                                                                                  gCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCt                                                                                      >  4:145981/1‑67 (MQ=255)
                                                                                                                                                  gCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCt                                                                                      <  3:145981/67‑1 (MQ=255)
                                                                                                                                                   ccGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGCTTCATc   >  4:230549/1‑149 (MQ=255)
                                                                                                                                                    cGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGCCGATGACCACCGAGCCGGGCTTCATCt  <  2:35999/149‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
CTCCTTAGCCGTTTTTGCGGACGACCTGACCATCACGGCACATCAGGCAGGCCGCGACGATGTCG‑‑‑TCTTCGAGGTTGATGATCAGCGCGCCATCCTTGTCGAACAGCAGCTTCATGAAGTCCAGCAGGTTGCGTGCGTACAGTGCCGAGGCATCGGCACTCACCTGGGCCGGCAGGTTGGTCGGGCCGACGATGATCACGCCGTTCTCCTGCACCACCTGGTCTGCGACGGTCAGCGGGCAGTTGCCGCCTTGGGCTGCAGCCAGGTCGATGACCACCGAGCCGGGCTTCATCT  >  NC_002947/165781‑166074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑