Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,506,930	T→C	12.2%	intergenic (+51/‑66)	petC → / → sspA	ubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,506,930	0	T	C	12.2%	99.5 / 6.0	41	intergenic (+51/‑66)	petC/sspA	ubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base T (20/16);  new base C (2/3);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AAGCCAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCC  >  NC_002947/1506783‑1507073                                                                                                                                                    |                                                                                                                                                aaGCCAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTAtt                                                                                                                                                <  8:107472/149‑1 (MQ=255)   gCCAGCGCATCGGCACCTATGTGTTTCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTTCTCAAGCGTGATTACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCTGTATTGc                                                                                                                                              <  8:297296/149‑1 (MQ=255)      aGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTGGCAATACTGCTAg                                                                                                                                           <  5:329168/149‑1 (MQ=255)      aGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAg                                                                                                                                           <  8:120079/149‑1 (MQ=255)        cgcATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGc                                                                                                                                         <  7:186583/149‑1 (MQ=255)        cgcATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGc                                                                                                                                         <  4:102909/149‑1 (MQ=255)            tCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTGGCAATACTGCTAGGCCAcc                                                                                                                                     <  4:277773/149‑1 (MQ=255)                  ccTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTGGCAATACTGCTAGGCCACCCCGGgc                                                                                                                               <  6:172673/149‑1 (MQ=255)                              tGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGgc                                                                                                                               <  1:158456/137‑1 (MQ=255)                              tGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGgc                                                                                                                               >  2:158456/1‑137 (MQ=255)                                  tCTGGCTTTCTTCTTCGTATACGCCTACTTGCTCAAGCGCTAATCCTGCAAGGACGTGCACTGATCACGCAGTCGTTTCTGGTTGTTCACCGCGCCCGGGGGGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTaaa                                                                                                                <  8:327147/148‑1 (MQ=255)                                        tttCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGc                                                                                                                                         <  4:145654/117‑1 (MQ=255)                                        tttCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGc                                                                                                                                         >  3:145654/1‑117 (MQ=255)                                            ttcttcTTATTCGCCTACTTGCACAAGCCTGAATACTGGAAGGACGTCCACTGATCACTCAGTAGTTTCTGGTTGTTGAACGCGCCCGGCGTGGCCTAGCACTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAAATGAACaaa                                                                                                      >  2:307643/1‑147 (MQ=255)                                            ttcttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTc                                                                                                                       <  7:310223/131‑1 (MQ=255)                                            ttcttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTc                                                                                                                       >  8:310223/1‑131 (MQ=255)                                              cttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGc                                                                                                     <  1:251751/147‑1 (MQ=255)                                                   tATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTg                                                                                              >  3:242185/1‑149 (MQ=255)                                                                      gCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATggg                                                                           >  6:212350/1‑149 (MQ=255)                                                                       cgttattACTGGAAGGACGTGCACTGATCACTGAGTTGTTTCTGGTTGTTGCTCGCTCCAGGGGTGGCCTAGCAGTATTCCCAGGCCACCCCGGGCGCGTTTTCATTAAAAGTTGCACATGCATCCGTGCGAGGAGCCGCTACATGGgc                                                                          <  1:307643/143‑1 (MQ=255)                                                                                               tGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGGTTTGATTTAAAGTTGGACCAGCATGCCTGCGAGGAGCCGGTACATGGGCGCCACCAACAGGTTAGCCTGCTAt                                                  >  6:31148/1‑149 (MQ=255)                                                                                                     cGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTCCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGAt                                            <  6:231496/149‑1 (MQ=255)                                                                                                      gCAGTAGGTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATc                                           <  5:31148/149‑1 (MQ=255)                                                                                                                 tGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCAc                                >  3:13029/1‑149 (MQ=255)                                                                                                                  ggTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATACCGCTGATCAc                                >  4:119062/1‑148 (MQ=255)                                                                                                                       ttgAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGGTTTCATTTAAAGTTGCGCAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATtct                          >  7:355146/1‑149 (MQ=255)                                                                                                                            cgcgCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCg                     >  5:305174/1‑149 (MQ=255)                                                                                                                               gcCCGGGGTGGCCTGGCAATACTGCTAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGt                  >  1:52037/1‑149 (MQ=255)                                                                                                                               gcCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTc                                                <  4:324437/119‑1 (MQ=255)                                                                                                                               gcCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTc                                                >  3:324437/1‑119 (MQ=255)                                                                                                                                cccGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGtt                 <  5:212350/149‑1 (MQ=255)                                                                                                                                         gCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGtt        >  7:23815/1‑149 (MQ=255)                                                                                                                                           cTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTTAAGTTGCACAAGCATACGTGCGAGGAGCCGCGACATGGGCGCAACCAAAAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCt      >  1:163701/1‑149 (MQ=255)                                                                                                                                           cTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGATGAGCCGCTACATGGGCCCAACCAACAGGTTAGCCTGCTATTACGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCt      >  3:190594/1‑149 (MQ=255)                                                                                                                                           cTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGcaaccaa                                                                  <  2:14874/89‑1 (MQ=255)                                                                                                                                           cTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGcaaccaa                                                                  >  1:14874/1‑89 (MQ=255)                                                                                                                                            tAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCt                                                       >  1:331981/1‑99 (MQ=255)                                                                                                                                            tAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCt                                                       <  2:331981/99‑1 (MQ=255)                                                                                                                                            tAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTg     >  2:160126/1‑149 (MQ=255)                                                                                                                                              gCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGc   >  5:240519/1‑149 (MQ=255)                                                                                                                                              gCAATACTGCTAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTGCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGc   >  1:343496/1‑149 (MQ=255)                                                                                                                                               cAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGcc  >  8:254379/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                AAGCCAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGGCCTAGCAGTATTGCCAGGCCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCC  >  NC_002947/1506783‑1507073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑