Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,508,753 G→A 6.4% A178V (GCG→GTG)  diaA ← replication/archosome assembly modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,508,7530GA 6.4% 231.3 / 2.9 77A178V (GCG→GTG) diaAreplication/archosome assembly modulator
Reads supporting (aligned to +/- strand):  ref base G (40/32);  new base A (3/2);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

GATCGGCGAATTGCGGGTAGAGGTGAGTACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACGGTCATGTGCGGCCTGG  >  NC_002947/1508606‑1508899
                                                                                                                                                   |                                                                                                                                                  
gATCGGCGAATTGCGGGTAGAGGTGAGTACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCCGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGc                                                                                                                                                   >  2:93130/1‑149 (MQ=255)
      cGAATTGCGGGTAGAGGTGAGTACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCcagcag                                                                                                                                             <  1:216327/149‑1 (MQ=255)
         aTTGCGGGTAGAGGTGAGTACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTg                                                                                                                                          <  2:305334/149‑1 (MQ=255)
                   gagGTGAGTACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTg                                                                                                                                 <  5:180175/148‑1 (MQ=255)
                           tACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTg                                                                                                                                          <  8:280072/131‑1 (MQ=255)
                           tACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTg                                                                                                                                          >  7:280072/1‑131 (MQ=255)
                                    gCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGt                                                                                                               >  6:359493/1‑149 (MQ=255)
                                    gCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGt                                                                                                               >  2:351793/1‑149 (MQ=255)
                                      aaCCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTc                                                                                                              >  2:216278/1‑148 (MQ=255)
                                        ccGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTCCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCg                                                                                                             >  8:152287/1‑147 (MQ=255)
                                              aCGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCAc                                                                                                      <  4:134761/148‑1 (MQ=255)
                                               cGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCAcgc                                                                                                    <  4:270739/149‑1 (MQ=255)
                                                gCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCAcgcg                                                                                                   >  1:350687/1‑149 (MQ=255)
                                                gCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCAcgcg                                                                                                   >  4:284385/1‑149 (MQ=255)
                                                   cAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGAt                                                                                                >  6:273166/1‑149 (MQ=255)
                                                    aGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGAtt                                                                                               >  8:189379/1‑149 (MQ=255)
                                                     ggCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGAttt                                                                                              <  4:88980/149‑1 (MQ=255)
                                                      gCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGTCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGa                                                                                                            <  1:290107/134‑1 (MQ=255)
                                                      gCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTc                                                                                             <  5:48930/149‑1 (MQ=255)
                                                      gCAAAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCATCAATCCCGAAAAGTTGCCTGTCAATCAGATCTAACAGGCACTGGATCACCAGAAGGTGCACATCCTGGATACGCGCGGTAAATGTCGa                                                                                                            >  2:290107/1‑134 (MQ=255)
                                                       cacaGGGGCAGGGCCATCAGGCCGAGGCGGATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGGCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCGTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTcc                                                                                            <  3:243286/149‑1 (MQ=255)
                                                        acaGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCa                                                                                           >  7:41890/1‑149 (MQ=255)
                                                        acaGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCa                                                                                           >  6:15976/1‑149 (MQ=255)
                                                         caGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCAc                                                                                          <  6:114306/149‑1 (MQ=255)
                                                            ggTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTc                                                                                       <  8:41890/149‑1 (MQ=255)
                                                               cAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTc                                                                                    >  7:201167/1‑149 (MQ=255)
                                                                aGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCg                                                                                   <  5:15976/149‑1 (MQ=255)
                                                                  ggCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 <  7:189379/149‑1 (MQ=255)
                                                                  ggCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 >  4:344936/1‑149 (MQ=255)
                                                                  ggCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 <  7:259622/149‑1 (MQ=255)
                                                                  ggCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 >  5:12514/1‑149 (MQ=255)
                                                                  ggCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 >  8:346974/1‑149 (MQ=255)
                                                                   gCCATCAGGCCGAGGCGCATAGGGGTCAATCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 >  1:218461/1‑148 (MQ=255)
                                                                      aTCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGgcagc                                                                             >  1:384707/1‑149 (MQ=255)
                                                                       tCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACTTCCTCGGgcag                                                                              >  8:351199/1‑147 (MQ=255)
                                                                       tCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGgcagca                                                                            >  1:220673/1‑149 (MQ=255)
                                                                         aGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACg                                                                                         <  7:261106/134‑1 (MQ=255)
                                                                         aGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACg                                                                                         >  8:261106/1‑134 (MQ=255)
                                                                           gCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGcagcagcag                                                                        >  7:148294/1‑149 (MQ=255)
                                                                            ccGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCagcagcagc                                                                       <  6:47105/149‑1 (MQ=255)
                                                                            ccGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCagcagcagc                                                                       <  2:218461/149‑1 (MQ=255)
                                                                                 gcgcATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGCTCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 >  3:235561/1‑134 (MQ=255)
                                                                                 gcgcATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCggg                                                                                 <  4:235561/134‑1 (MQ=255)
                                                                                        ggggTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATgccg                                                           <  6:12514/149‑1 (MQ=255)
                                                                                           gTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGGAACTGTCGAAGGCCCGCGGATTTCCACGTCCGCGGGCAGCGGCCGCGAAGCCATgccgccg                                                        >  8:282224/1‑149 (MQ=255)
                                                                                           gTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGAAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATgccgccg                                                        >  2:169395/1‑149 (MQ=255)
                                                                                            tCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATgccgccgc                                                       >  5:80173/1‑149 (MQ=255)
                                                                                                    cACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTcg                                                  >  5:282610/1‑146 (MQ=255)
                                                                                                    cACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTcg                                                  <  6:282610/146‑1 (MQ=255)
                                                                                                     aCTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGa                                               <  6:80173/148‑1 (MQ=255)
                                                                                                     aCTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGa                                               <  7:346974/148‑1 (MQ=255)
                                                                                                       tCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGAccc                                            >  6:188184/1‑149 (MQ=255)
                                                                                                         ccGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGaa                                                                    <  5:42340/123‑1 (MQ=255)
                                                                                                         ccGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGaa                                                                    >  6:42340/1‑123 (MQ=255)
                                                                                                         ccGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGt                                          >  3:47396/1‑149 (MQ=255)
                                                                                                         ccGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCACCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTcc                                                                                            >  3:89885/1‑99 (MQ=255)
                                                                                                         ccGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCACCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTcc                                                                                            <  4:89885/99‑1 (MQ=255)
                                                                                                           gAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGGGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTc                                         >  1:220138/1‑148 (MQ=255)
                                                                                                            aaCAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCCCGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGACATGACGCCGCCGACGCGACCCGTCaa                                       >  5:230094/1‑149 (MQ=255)
                                                                                                                      tGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACATCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGa                               >  6:96538/1‑147 (MQ=255)
                                                                                                                      tGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACATCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGa                               <  5:96538/147‑1 (MQ=255)
                                                                                                                        tCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAAt                           <  1:351793/149‑1 (MQ=255)
                                                                                                                          gATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATc                          <  3:284385/148‑1 (MQ=255)
                                                                                                                          gATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACAGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATCCTACGACAATCa                         >  7:313892/1‑149 (MQ=255)
                                                                                                                           aTCAGATCGCACAGGCAGTGGATCGCCAGCAGGTCGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCGCGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGAGAATCAt                        >  1:226561/1‑149 (MQ=255)
                                                                                                                            tCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCAtt                       >  1:84041/1‑149 (MQ=255)
                                                                                                                              aGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCAttt                      <  5:359493/148‑1 (MQ=255)
                                                                                                                               gATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCa                    <  3:344936/149‑1 (MQ=255)
                                                                                                                               gATCGCACAGGCAGTGGATCACCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGt                                          >  6:367615/1‑127 (MQ=255)
                                                                                                                               gATCGCACAGGCAGTGGATCACCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGt                                          <  5:367615/127‑1 (MQ=255)
                                                                                                                                  cGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACgg                 >  6:41324/1‑149 (MQ=255)
                                                                                                                                  cGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACgg                 >  6:35844/1‑149 (MQ=255)
                                                                                                                                           aGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCATGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACGGTCATGTGCg        >  4:112534/1‑149 (MQ=255)
                                                                                                                                               gATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATgcc                                                            >  6:351747/1‑93 (MQ=255)
                                                                                                                                               gATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATgcc                                                            <  5:351747/93‑1 (MQ=255)
                                                                                                                                                 tCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACGGTCATGTGCggcctgg  <  5:41324/149‑1 (MQ=255)
                                                                                                                                                 tCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACGGTCATGTGCggcctgg  <  5:35844/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCGGCGAATTGCGGGTAGAGGTGAGTACCGAGCTGCAACCGGTGACGCTCAGGCACAGGGTCAGGGCCATCAGGCCGAGGCGCATAGGGGTCATTCTTCACTCCCGAACAGTTGGCTGTCGATCAGATCGCACAGGCAGTGGATCGCCAGCAGGTGGACTTCCTGGATACGCGCGGTAACTGTCGAAGGCACGCGGATTTCCACGTCCTCGGGCAGCAGCAGCGAAGCCATGCCGCCGCCGTCGCGACCCGTCAATGCTACGACAATCATTTCACGGTCATGTGCGGCCTGG  >  NC_002947/1508606‑1508899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑