Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,564,931 A→T 7.1% L133Q (CTG→CAG)  pitB ← phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,564,9310AT 7.1% 204.2 / 3.3 69L133Q (CTG→CAG) pitBphosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base T (3/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

CGGGCGCCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATCAGCAG  >  NC_002947/1564783‑1565079
                                                                                                                                                    |                                                                                                                                                    
cGGGCGCCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGGCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTggcca                                                                                                                                                      <  5:58861/149‑1 (MQ=255)
    cgcCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTggccaggcc                                                                                                                                                  <  1:151143/149‑1 (MQ=255)
       caccaTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCaa                                                                                                                                                >  2:338887/1‑148 (MQ=255)
        accaTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAaca                                                                                                                                              >  7:57983/1‑149 (MQ=255)
          caTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCAATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACAcc                                                                                                                                            <  2:245183/149‑1 (MQ=255)
           aTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCTGGCCAACGccc                                                                                                                                           <  4:38733/149‑2 (MQ=255)
              tCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAg                                                                                                                                         <  7:327243/148‑1 (MQ=255)
                aGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGAt                                                                                                                                      >  6:336417/1‑149 (MQ=255)
                aGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGAt                                                                                                                                      <  8:5767/149‑1 (MQ=255)
                  gCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATg                                                                                                                                     >  7:207940/1‑148 (MQ=255)
                    cGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGaa                                                                                                                                  >  2:122333/1‑149 (MQ=255)
                    cGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGaa                                                                                                                                  <  2:352912/149‑1 (MQ=255)
                       tCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAAcc                                                                                                                                >  5:39930/1‑148 (MQ=255)
                       tCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCg                                                                                                                               <  1:7994/149‑1 (MQ=255)
                           cACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCa                                                                                                                           <  1:338887/149‑1 (MQ=255)
                               aGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCgt                                                                                                                       <  8:72908/149‑1 (MQ=255)
                                  gcagcaACGGCGAAGCCGGCCATTGGCGAGACAACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCgtgt                                                                                                                     <  5:389648/148‑1 (MQ=255)
                                   cagcaACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGc                                                                                                                   >  3:45815/1‑149 (MQ=255)
                                      caACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGaa                                                                                                                >  1:10565/1‑149 (MQ=255)
                                                gCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCgg                                                                                                      <  1:162059/149‑1 (MQ=255)
                                                 ccGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCggg                                                                                                     >  8:38068/1‑149 (MQ=255)
                                                 ccGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCggg                                                                                                     >  7:154069/1‑149 (MQ=255)
                                                    gCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGAt                                                                                                  <  2:370763/149‑1 (MQ=255)
                                                     ccATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATg                                                                                                 >  6:1819/1‑149 (MQ=255)
                                                      cATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGc                                                                                                <  6:39930/149‑1 (MQ=255)
                                                           gCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTgg                                                                                                            >  4:10624/1‑132 (MQ=255)
                                                           gCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTAGACGCCGTCGCCCAGCGGAATGTCTTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAg                                                                                           >  1:273239/1‑149 (MQ=255)
                                                            cGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGa                                                                                                                 <  4:106029/126‑1 (MQ=255)
                                                            cGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGa                                                                                                                 >  3:106029/1‑126 (MQ=255)
                                                             gagaCCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTa                                                                                         <  4:45815/149‑1 (MQ=255)
                                                                accaccAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTAc                                                                                        >  1:139244/1‑147 (MQ=255)
                                                                   accaGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGt                                                                                   <  3:45437/149‑1 (MQ=255)
                                                                     caGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGc                                                                                 <  5:221236/149‑1 (MQ=255)
                                                                     caGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGc                                                                                 >  4:164328/1‑149 (MQ=255)
                                                                       gCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGccc                                                                               <  2:10565/149‑1 (MQ=255)
                                                                        cGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGcc                                                                                >  4:252717/1‑147 (MQ=255)
                                                                            aTGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTGGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGtt                                                                          >  6:57514/1‑149 (MQ=255)
                                                                                ctatatCTATCGCTTTCTGCCTGTTGACGCCGTCGCCCAGCGTAATGTCTTTGATCTGGTCGTTGGCATGGCAAAAACTTAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAg                                                                      <  1:122333/147‑1 (MQ=255)
                                                                                       gatcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATg                                                                <  5:336417/148‑1 (MQ=255)
                                                                                       gatcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCAAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATgg                                                               >  5:125477/1‑149 (MQ=255)
                                                                                        atcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGc                                                              >  7:175062/1‑149 (MQ=255)
                                                                                         tcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGcc                                                             >  3:309958/1‑149 (MQ=255)
                                                                                             tttCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAg                                                                      >  5:32800/1‑136 (MQ=255)
                                                                                             tttCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAg                                                                      <  6:32800/136‑1 (MQ=255)
                                                                                                cTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGcagcc                                                       <  8:154069/148‑1 (MQ=255)
                                                                                                 tGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGcagccag                                                     >  3:103705/1‑149 (MQ=255)
                                                                                                   ccAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGc                                                                                                >  5:291107/1‑104 (MQ=255)
                                                                                                   ccAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGc                                                                                                <  6:291107/104‑1 (MQ=255)
                                                                                                    cAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCCGCCAGCaa                                                  >  8:197730/1‑149 (MQ=255)
                                                                                                      gTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGcc                                                             >  5:372299/1‑136 (MQ=255)
                                                                                                      gTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGcc                                                             <  6:372299/136‑1 (MQ=255)
                                                                                                         gACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACgag                                              <  8:57983/148‑1 (MQ=255)
                                                                                                                 cGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATg                                     <  3:252717/149‑1 (MQ=255)
                                                                                                                     cAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGc                                   >  4:375471/1‑147 (MQ=255)
                                                                                                                       gCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGTGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAgg                               <  5:380558/149‑1 (MQ=255)
                                                                                                                          gAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCg                            >  6:114031/1‑149 (MQ=255)
                                                                                                                                 gTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCgg                     >  5:96612/1‑149 (MQ=255)
                                                                                                                                 gTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCgg                     >  5:91204/1‑149 (MQ=255)
                                                                                                                                 cttGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCg                      <  2:273239/147‑1 (MQ=255)
                                                                                                                                  ttGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGgt                    <  4:3313/149‑1 (MQ=255)
                                                                                                                                   tGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGgt                    <  8:175062/148‑1 (MQ=255)
                                                                                                                                      tCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTc                <  2:246047/149‑1 (MQ=255)
                                                                                                                                      tCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTc                <  5:57514/149‑1 (MQ=255)
                                                                                                                                        tcggtgTTGGCCTGGCCAACGCCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTca               >  8:10640/6‑148 (MQ=255)
                                                                                                                                        tcggtgTTGGCCTGGCCAACGCCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTca               >  1:232400/6‑148 (MQ=255)
                                                                                                                                        tcggtgTTGGCCTGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTca               >  7:248617/6‑148 (MQ=255)
                                                                                                                                        aGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTca               >  6:116858/1‑148 (MQ=255)
                                                                                                                                         gggCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTcaca             <  6:96612/149‑1 (MQ=255)
                                                                                                                                         gggCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTcaca             <  6:91204/149‑1 (MQ=255)
                                                                                                                                         gggCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTcaca             <  5:1819/149‑1 (MQ=255)
                                                                                                                                          ggCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGATCTGGAGGCGGGGATGGCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCGAGGAACGAGAAGCCCATGGCCAGGCCGTGGACGGTGTTcacag            >  4:261765/1‑148 (MQ=255)
                                                                                                                                                 gccaggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATcag     >  4:119480/1‑149 (MQ=255)
                                                                                                                                                   caggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAg                                                                      <  7:1120/82‑1 (MQ=255)
                                                                                                                                                   caggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAg                                                                      >  8:1120/1‑82 (MQ=255)
                                                                                                                                                    aggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATctgcag  >  4:340486/1‑149 (MQ=255)
                                                                                                                                                    aggccaACACCCACGATGGAACCGATCAGCGTGTGCGAACTGCAGGCGGGGAAGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGGAACGCGAAGACCCTGGCCAGGCCGTGGCCGGTGTTCCAATAGATcagcag  >  4:77294/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGGCGCCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATCAGCAG  >  NC_002947/1564783‑1565079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑