Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,631,095 C→A 5.8% intergenic (+29/‑152) PP_1430 → / → lepA DegP‑like serine endoprotease/elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,631,0950CA 5.8% 208.2 / 3.5 69intergenic (+29/‑152)PP_1430/lepADegP‑like serine endoprotease/elongation factor 4
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base A (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

GTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGTT  >  NC_002947/1630951‑1631241
                                                                                                                                                |                                                                                                                                                  
gTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCt                                                                                                                                                >  8:117861/1‑149 (MQ=255)
    ggCCAAGGAATTCACCGAATTCGCGAAGGAACTGGCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTGAAGCTTGCTGAATAAGCTGTTTCGGAAGTCGAAATGGCAGCTTGGGCTGcc                                                                                                                                             <  6:1414/148‑1 (MQ=255)
        aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCtttt                                                                                                                                        >  8:303970/1‑149 (MQ=255)
        aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttc                                                                                                                                        >  1:292251/1‑148 (MQ=255)
          ggAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTc                                                                                                                                      <  5:149200/149‑1 (MQ=255)
           gAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttcgcc                                                                                                                                     <  2:292251/149‑5 (MQ=255)
            aaTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTTCCCTTTTTCAt                                                                                                                                    <  6:282402/149‑1 (MQ=255)
            aaTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCAt                                                                                                                                    <  7:303970/149‑1 (MQ=255)
            aaTTCACCGAAATCACCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTCCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCAt                                                                                                                                    <  1:123682/149‑1 (MQ=255)
             aTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttcgcctt                                                                                                                                   <  8:14350/149‑7 (MQ=255)
                 aCCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATc                                                                                                                               >  7:346423/1‑149 (MQ=255)
                  ccGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATc                                                                                                                               <  1:104778/148‑1 (MQ=255)
                  ccGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATc                                                                                                                               >  2:104778/1‑148 (MQ=255)
                   cGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCtt                                                                                                                             >  3:357961/1‑149 (MQ=255)
                    gcaaTCGCCAAGGACCTGCCGAAGAATCGTTCCGTGTCGAGGCGCGTACTGCGGCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTc                                                                                                                            <  8:83380/147‑1 (MQ=255)
                      aaTCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTcac                                                                                                                          <  5:149983/149‑1 (MQ=255)
                            cAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGc                                                                                                                    >  7:4858/1‑149 (MQ=255)
                             aaGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGcc                                                                                                                   >  8:37578/1‑149 (MQ=255)
                                                 ttCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttttt                                                                                                                                       <  2:258507/109‑1 (MQ=255)
                                                 ttCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttttt                                                                                                                                       >  1:258507/1‑109 (MQ=255)
                                                       gtCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGa                                                                                          >  4:232134/1‑148 (MQ=255)
                                                        tCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGa                                                                                          >  8:191046/1‑147 (MQ=255)
                                                         cGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGc                                                                                                                    <  5:219725/120‑1 (MQ=255)
                                                         cGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGc                                                                                                                    >  6:219725/1‑120 (MQ=255)
                                                          gATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACaga                                                                                      <  5:204745/149‑1 (MQ=255)
                                                           aTGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACagag                                                                                     <  6:258611/149‑1 (MQ=255)
                                                               gcgTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGaa                                                                                  >  1:136493/1‑148 (MQ=255)
                                                                   aCTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACaga                                                                                      <  7:180685/140‑1 (MQ=255)
                                                                   aCTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACaga                                                                                      >  8:180685/1‑140 (MQ=255)
                                                                       cGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCtgt                                                                                                     <  6:56509/121‑1 (MQ=255)
                                                                       cGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCtgt                                                                                                     >  5:56509/1‑121 (MQ=255)
                                                                        gTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATaa                                                                        >  2:238127/1‑149 (MQ=255)
                                                                        gTCAGGGCCGTGCCAGATTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCGTTTTTCATGAATCTTCACAATTGCCGGAATGAGTGCTGTGTACAGTCGGACAGAGGAATCGCCAATaa                                                                        >  8:266223/1‑149 (MQ=255)
                                                                            gggCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCca                                                                    <  5:335856/149‑1 (MQ=255)
                                                                             ggCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCcag                                                                   >  2:269626/1‑149 (MQ=255)
                                                                               ccGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCcag                                                                   >  3:189452/1‑147 (MQ=255)
                                                                                cGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCcagcag                                                                <  8:118279/149‑1 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                          <  7:191046/149‑1 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                          <  4:357961/149‑1 (MQ=255)
                                                                                      aGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                          >  7:161449/1‑149 (MQ=255)
                                                                                       gCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAgg                                                         <  7:117861/149‑1 (MQ=255)
                                                                                              caCTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                          <  1:359434/141‑1 (MQ=255)
                                                                                              caCTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAg                                                          >  2:359434/1‑141 (MQ=255)
                                                                                                cTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAAt                                                                                 <  6:210841/116‑1 (MQ=255)
                                                                                                cTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAAt                                                                                 >  5:210841/1‑116 (MQ=255)
                                                                                                 tttCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTccc                                               >  7:140647/1‑149 (MQ=255)
                                                                                                       gCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAAcc                                                                      <  6:80634/120‑1 (MQ=255)
                                                                                                       gCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGAATGCTGTGTACAGTCGGACAGAGGAATCTCCCATAAcc                                                                      >  5:80634/1‑120 (MQ=255)
                                                                                                          tGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGAAGCTTAAGGTACAATTCCCGGCTAtttt                                      >  7:236671/1‑149 (MQ=255)
                                                                                                           gCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTAttt                                       <  2:136493/147‑1 (MQ=255)
                                                                                                                   aGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCggcggg                              <  4:189452/148‑1 (MQ=255)
                                                                                                                    gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCggcgggcg                            >  8:50784/1‑149 (MQ=255)
                                                                                                                     cAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGTCGGGCGt                           >  8:43493/1‑149 (MQ=255)
                                                                                                                      aGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCTTc                          <  3:232134/149‑1 (MQ=255)
                                                                                                                           cGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCt                     >  4:299810/1‑149 (MQ=255)
                                                                                                                               aGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCg                   >  2:333241/1‑147 (MQ=255)
                                                                                                                                   gAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTAGGCGGGCGTCCGGCTCGCAGCCt             >  5:115907/1‑149 (MQ=255)
                                                                                                                                   aaaaGGGCAGCCGAAGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCt             >  4:328609/2‑149 (MQ=255)
                                                                                                                                    aaaGGGCAGCTTCGGCTGCCGTTTTTCATGAATCTTCACAATTGGCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATGTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCtt            >  4:230046/1‑149 (MQ=255)
                                                                                                                                     aaGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTccc                                                                            <  7:167110/84‑1 (MQ=255)
                                                                                                                                     aaGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTccc                                                                            >  8:167110/1‑84 (MQ=255)
                                                                                                                                        ggCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTc         >  3:378632/1‑148 (MQ=255)
                                                                                                                                         gCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGa       <  8:4858/149‑1 (MQ=255)
                                                                                                                                         gCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGa       <  1:238127/149‑1 (MQ=255)
                                                                                                                                          cAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAg      <  8:161449/149‑1 (MQ=255)
                                                                                                                                           aGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATACCCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGTCGGGCGTCCGGCTCGCAGCCTTTTCGAgt     >  8:210731/1‑149 (MQ=255)
                                                                                                                                           aGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAgt     <  1:333241/149‑1 (MQ=255)
                                                                                                                                              ttCGGCTGCCCTTTTTCTTGATTCTTCACAATTGCGGGTATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATTACCCAGCAGCTTAAGGTAGATTTCCCGGGTATTTTTCGGAGGGCGTCCGGCTGGCTGGCTTTTGGAGTGtt  >  4:319544/1‑149 (MQ=255)
                                                                                                                                              ttCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGtt  <  7:266223/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGTGTT  >  NC_002947/1630951‑1631241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑