Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,661,477	C→A	9.3%	A50D (GCC→GAC)	PP_1456 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,661,477	0	C	A	9.3%	193.3 / 7.9	64	A50D (GCC→GAC)	PP_1456	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base A (3/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

ATGTTCTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGA  >  NC_002947/1661329‑1661618                                                                                                                                                     |                                                                                                                                              aTGTTCTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGgcgc                                                                                                                                               <  5:40749/149‑1 (MQ=255)        aTCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                        >  8:113906/1‑149 (MQ=255)         tCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                        <  3:88796/148‑1 (MQ=255)         tCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                        <  6:366711/148‑1 (MQ=255)         tCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                        <  3:146244/148‑1 (MQ=255)             aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCg                                                                                                                                       <  3:129521/145‑1 (MQ=255)                 ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtg                                                                                                                               <  6:346999/149‑1 (MQ=255)                 ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATgac                                                                                                                                <  8:64189/148‑3 (MQ=255)                 ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATgac                                                                                                                                <  2:308499/148‑3 (MQ=255)                     cATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggtgg                                                                                                                           >  2:97125/1‑149 (MQ=255)                     cATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggtg                                                                                                                            <  6:41725/148‑1 (MQ=255)                          gcagcGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTg                                                                                                                      >  1:136613/1‑149 (MQ=255)                          gcagcGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTg                                                                                                                      <  1:97125/149‑1 (MQ=255)                            agcGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGt                                                                                                                    <  2:290751/149‑1 (MQ=255)                                  gTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGGCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGt                                                                                                               <  4:29991/148‑1 (MQ=255)                                    gCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGGTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCt                                                                                                            <  4:281066/149‑1 (MQ=255)                                        aTCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTAt                                                                                                          <  5:101772/147‑1 (MQ=255)                                        aTCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTAt                                                                                                          <  7:113906/147‑1 (MQ=255)                                         tCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTc                                                                                                       <  2:119231/149‑1 (MQ=255)                                            aCTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTg                                                                                                                      <  8:14865/131‑1 (MQ=255)                                            aCTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTg                                                                                                                      >  7:14865/1‑131 (MQ=255)                                            aCTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGGCCTATGTCAcc                                                                                                    >  6:289905/1‑149 (MQ=255)                                              tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTc                                                                                                  >  5:386774/1‑149 (MQ=255)                                                cGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGt                                                                                                               <  5:390/134‑1 (MQ=255)                                                cGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGt                                                                                                               >  6:390/1‑134 (MQ=255)                                                cGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGATGTCCTATGTCACCTCg                                                                                                 >  6:268510/1‑148 (MQ=255)                                                 gCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                 >  8:221323/1‑147 (MQ=255)                                                        ccAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGCCCTATGTCACCTCGGAGCCGGc                                                                                         >  2:202350/1‑148 (MQ=255)                                                               cccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCTGAGCCGGCCATCGccc                                                                                 >  6:316488/1‑149 (MQ=255)                                                                 cGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTGTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTg                                                                               <  1:87161/149‑1 (MQ=255)                                                                   cATCTGTACATCAGCTGGTCTGCCTGTGTCGGCCTGGTGTTCATGCTGCGCGACATTGTGCAGACCCGTTTTGGTCATGGGGCCCTGGTCGCGATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGTGCCGGCCATCTCCCTTGc                                                                             <  4:134838/149‑1 (MQ=255)                                                                      cTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAg                                                                          >  3:199708/1‑149 (MQ=255)                                                                             tcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACt                                                                   >  1:350188/1‑149 (MQ=255)                                                                                tcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCg                                                                >  8:225215/1‑149 (MQ=255)                                                                                    gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGTCACTGGTGCTGTCCTATTTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGt                                                               <  5:268510/146‑1 (MQ=255)                                                                                       tGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCt                                                                                                                 >  8:65986/1‑93 (MQ=255)                                                                                       tGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCt                                                                                                                 <  7:65986/93‑1 (MQ=255)                                                                                       tGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCa                                                         >  3:69157/1‑149 (MQ=255)                                                                                         ccTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttcttc                                                          <  7:221323/146‑1 (MQ=255)                                                                                          cTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCAttt                                                      >  7:269423/1‑149 (MQ=255)                                                                                            ggggCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTcc                                                    <  2:51016/149‑1 (MQ=255)                                                                                               gcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAg                                                 <  1:142583/149‑1 (MQ=255)                                                                                                    cTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGAt                                            <  6:199048/149‑1 (MQ=255)                                                                                                     tGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATc                                           >  3:156057/1‑149 (MQ=255)                                                                                                        tgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAc                                        <  4:199708/149‑1 (MQ=255)                                                                                                        tgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAc                                        <  7:235798/149‑1 (MQ=255)                                                                                                          ttCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCNAGCGCCACTGCGTTCTTCATNTCCGAGNTGATCGActg                                      >  2:299792/1‑149 (MQ=255)                                                                                                          ttCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActg                                      >  2:294867/1‑149 (MQ=255)                                                                                                             aTCCTGCGCAACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggct                                   >  2:50315/1‑149 (MQ=255)                                                                                                              tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                  <  2:136613/149‑1 (MQ=255)                                                                                                               ccTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctgg                                 >  3:38950/1‑149 (MQ=255)                                                                                                                     cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTACTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                           <  3:330264/149‑1 (MQ=255)                                                                                                                         aTGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGCCTGGCTGGTGTTCAGCAt                       >  4:380286/1‑149 (MQ=255)                                                                                                                           ggTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCa                     >  4:148578/1‑149 (MQ=255)                                                                                                                                 gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc               <  1:50315/149‑1 (MQ=255)                                                                                                                                   cccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCgg             <  6:386774/149‑1 (MQ=255)                                                                                                                                    ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGc            >  2:27934/1‑149 (MQ=255)                                                                                                                                      tccgatctCATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGc            <  5:108292/140‑1 (MQ=255)                                                                                                                                           cagcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCag                    >  2:81002/4‑133 (MQ=255)                                                                                                                                            gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc    <  1:27934/149‑1 (MQ=255)                                                                                                                                             tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAc                                        <  2:286793/112‑1 (MQ=255)                                                                                                                                             tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAc                                        >  1:286793/1‑112 (MQ=255)                                                                                                                                             tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGcgcg   >  2:57281/1‑149 (MQ=255)                                                                                                                                              cATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa  >  4:22640/1‑149 (MQ=255)                                                                                                                                               aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGc            >  6:108292/1‑138 (MQ=255)                                                                                                                                                     |                                                                                                                                              ATGTTCTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGA  >  NC_002947/1661329‑1661618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑