Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,684,272 T→C 7.3% F284S (TTT→TCT)  PP_1478 → xenobiotic reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,684,2720TC 7.3% 233.5 / 6.9 82F284S (TTT→TCT) PP_1478xenobiotic reductase
Reads supporting (aligned to +/- strand):  ref base T (39/37);  new base C (3/3);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

GACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCC  >  NC_002947/1684124‑1684419
                                                                                                                                                    |                                                                                                                                                   
gactgGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCtt                                                                                                                                                     >  2:56926/1‑149 (MQ=255)
    ggggCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCa                                                                                                                                                 <  4:282137/149‑1 (MQ=255)
     ggTCGAGGTCGGCTGGAACCCGGATGAAACCGTCGAACTGGCTAGTCGCCTGCGGGTGCTGGGTTTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCACTGGCTATCAGACGCGCTTTg                                                                                                                                                   <  3:210746/146‑1 (MQ=255)
     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCACCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTg                                                                                                                                                   >  6:273894/1‑146 (MQ=255)
     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCa                                                                                                                                                 <  8:93682/148‑1 (MQ=255)
     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCa                                                                                                                                                 <  2:99129/148‑1 (MQ=255)
         gAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAgcg                                                                                                                                            >  2:257966/1‑149 (MQ=255)
          aGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAgcgc                                                                                                                                           >  2:205260/1‑149 (MQ=255)
          aGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAgagc                                                                                                                                           >  2:354114/1‑149 (MQ=255)
            gACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGt                                                                                                                                         <  3:294820/149‑1 (MQ=255)
            gACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGt                                                                                                                                         <  3:214155/149‑1 (MQ=255)
             aCGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGtc                                                                                                                                        <  5:367398/149‑2 (MQ=255)
              cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgc                                                                                                                                       <  4:249684/149‑1 (MQ=255)
              cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgc                                                                                                                                       <  6:361338/149‑1 (MQ=255)
               ggCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgcg                                                                                                                                      >  1:13769/1‑149 (MQ=255)
                      aCCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAgg                                                                                                                                 >  4:259021/1‑147 (MQ=255)
                         cGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCa                                                                                                                            <  5:244239/149‑1 (MQ=255)
                               aaaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGAt                                                                                                                       <  1:257966/148‑1 (MQ=255)
                               aaaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATc                                                                                                                      <  6:167338/149‑1 (MQ=255)
                               aaaCCGTCGAACTGGCTAGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGGCCTCCTGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGAt                                                                                                                       <  3:238538/148‑1 (MQ=255)
                                aaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTTCATGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCg                                                                                                                     <  5:273894/149‑1 (MQ=255)
                                aaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATc                                                                                                                      <  5:47047/148‑1 (MQ=255)
                                aaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCg                                                                                                                     >  2:1483/1‑149 (MQ=255)
                                       gAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCg                                                                                                               <  3:124055/148‑1 (MQ=255)
                                             gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGc                                                                                                                    <  6:372162/137‑1 (MQ=255)
                                             gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGc                                                                                                                    >  5:372162/1‑137 (MQ=255)
                                             gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGt                                                                                                        >  2:123000/1‑149 (MQ=255)
                                                     ccTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGa                                                                                                >  1:189584/1‑149 (MQ=255)
                                                      ccggatctGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATg                                                                                                 <  5:314600/140‑1 (MQ=255)
                                                        gCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                             >  7:279364/1‑149 (MQ=255)
                                                         ctggTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                             <  7:28652/146‑1 (MQ=255)
                                                         cGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                             <  8:279364/148‑1 (MQ=255)
                                                         cGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCAc                                                                                            <  7:164094/149‑1 (MQ=255)
                                                          gggTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACt                                                                                           <  1:334320/149‑1 (MQ=255)
                                                               cTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAAc                                                                                       <  1:341126/148‑1 (MQ=255)
                                                               cTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAAc                                                                                       <  3:259021/148‑1 (MQ=255)
                                                               cTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGGCGGCATg                                                                                                 >  6:314600/1‑138 (MQ=255)
                                                                  ggTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTc                                                                                                       >  4:355659/1‑129 (MQ=255)
                                                                  ggTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTc                                                                                                       <  3:355659/129‑1 (MQ=255)
                                                                      tCGACCTGATCGAAGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCa                                                                                <  3:37326/148‑1 (MQ=255)
                                                                        gACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGc                                                                             >  4:234239/1‑149 (MQ=255)
                                                                         aCCTGATCGATGTGTCCTCCGGTTGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTCGCTATCAGACGCGATTTGAAGAGCGCGTGCGCAAGGAAGCa                                                                                                                            >  4:121906/1‑101 (MQ=255)
                                                                         aCCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCa                                                                                                                            <  3:121906/101‑1 (MQ=255)
                                                                         aCCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                            >  2:39652/1‑149 (MQ=255)
                                                                          ccTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                            >  4:182161/1‑148 (MQ=255)
                                                                          ccTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                            >  7:167064/1‑148 (MQ=255)
                                                                           cTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                            >  7:51935/1‑147 (MQ=255)
                                                                                       tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                               <  8:167064/148‑1 (MQ=255)
                                                                                       tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                               <  1:39652/148‑1 (MQ=255)
                                                                                       tcctccGGTGGTACCTCGGTCAACCCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCg                                                              >  4:262457/1‑149 (MQ=255)
                                                                                         ctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTa                                                            >  2:67810/1‑149 (MQ=255)
                                                                                         ctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTa                                                            >  7:66642/1‑149 (MQ=255)
                                                                                           ccGGTGGTACCTCGGTCAACGCCGAACTTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTAcc                                                          >  1:12571/1‑149 (MQ=255)
                                                                                                  tACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAgg                                                   >  2:292827/1‑149 (MQ=255)
                                                                                                   aCCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGc                                                  >  5:42307/1‑149 (MQ=255)
                                                                                                   aCCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGc                                                  >  7:69156/1‑149 (MQ=255)
                                                                                                     cTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCACAGGCCGAGCACATTCTGCGTACCGGGCAGGcc                                                 >  8:166650/1‑148 (MQ=255)
                                                                                                      tCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGa                                               >  8:352474/1‑149 (MQ=255)
                                                                                                            aaCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCTGACGTGAt                                         >  8:126324/1‑149 (MQ=255)
                                                                                                            aaCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGa                                          <  8:51935/148‑1 (MQ=255)
                                                                                                             aCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATc                                        <  1:123000/149‑1 (MQ=255)
                                                                                                                 cGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTcc                                    >  5:312600/1‑149 (MQ=255)
                                                                                                                 cGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTcc                                    >  4:330239/1‑149 (MQ=255)
                                                                                                                  gAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCt                                   <  8:69156/149‑1 (MQ=255)
                                                                                                                      ttCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCt                               >  1:144274/1‑149 (MQ=255)
                                                                                                                         cTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                            <  7:166650/149‑1 (MQ=255)
                                                                                                                          tACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                            >  6:336147/1‑148 (MQ=255)
                                                                                                                          tACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                            >  5:201844/1‑148 (MQ=255)
                                                                                                                                 ccTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAgctgct                    >  1:117835/1‑149 (MQ=255)
                                                                                                                                   tGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCtgctgc                  >  4:300035/1‑149 (MQ=255)
                                                                                                                                        aTCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGAt             >  1:12031/1‑149 (MQ=255)
                                                                                                                                         tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGa              <  3:182161/147‑1 (MQ=255)
                                                                                                                                         tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGAt             <  2:13769/148‑1 (MQ=255)
                                                                                                                                         tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATc            <  1:205260/149‑1 (MQ=255)
                                                                                                                                          cAGACGCGCTTTGCATAGCGCGTGCGCAAGGAAGCATAGATCGCAACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATc            <  1:354114/148‑1 (MQ=255)
                                                                                                                                           gcaCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCATTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGCCGTGCTCTTCCTGGCGCGGGCGCAGCGGCGCGAGCCt          >  4:303795/3‑149 (MQ=255)
                                                                                                                                           gcaCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCATTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCt          >  6:342415/3‑149 (MQ=255)
                                                                                                                                           aGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCt          >  2:204414/1‑149 (MQ=255)
                                                                                                                                            caCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCt          >  8:159640/2‑148 (MQ=255)
                                                                                                                                              cgcgCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTAc       <  3:262457/149‑1 (MQ=255)
                                                                                                                                               gcgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAg                                                                         >  4:209409/1‑82 (MQ=255)
                                                                                                                                               gcgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAg                                                                         <  3:209409/82‑1 (MQ=255)
                                                                                                                                                cgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTg     <  7:352474/149‑1 (MQ=255)
                                                                                                                                                   tttGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGcc  >  7:53215/1‑149 (MQ=255)
                                                                                                                                                   tttGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGcc  >  3:256180/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCC  >  NC_002947/1684124‑1684419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑