Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,684,277	G→A	7.3%	E286K (GAG→AAG)	PP_1478 →	xenobiotic reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,684,277	0	G	A	7.3%	242.5 / 6.1	83	E286K (GAG→AAG)	PP_1478	xenobiotic reductase
Reads supporting (aligned to +/- strand):  ref base G (42/34);  new base A (3/3);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

GAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCTGCA  >  NC_002947/1684133‑1684425                                                                                                                                                 |                                                                                                                                                     gAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAgcg                                                                                                                                                  >  2:257966/1‑149 (MQ=255)  aGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAgcgc                                                                                                                                                 >  2:205260/1‑149 (MQ=255)  aGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAgagc                                                                                                                                                 >  2:354114/1‑149 (MQ=255)    gACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGt                                                                                                                                               <  3:294820/149‑1 (MQ=255)    gACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGt                                                                                                                                               <  3:214155/149‑1 (MQ=255)     aCGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGtc                                                                                                                                              <  5:367398/149‑2 (MQ=255)      cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgc                                                                                                                                             <  6:361338/149‑1 (MQ=255)      cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgc                                                                                                                                             <  4:249684/149‑1 (MQ=255)       ggCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgcg                                                                                                                                            >  1:13769/1‑149 (MQ=255)              aCCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAgg                                                                                                                                       >  4:259021/1‑147 (MQ=255)                 cGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCa                                                                                                                                  <  5:244239/149‑1 (MQ=255)                       aaaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGAt                                                                                                                             <  1:257966/148‑1 (MQ=255)                       aaaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATc                                                                                                                            <  6:167338/149‑1 (MQ=255)                       aaaCCGTCGAACTGGCTAGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGGCCTCCTGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGAt                                                                                                                             <  3:238538/148‑1 (MQ=255)                        aaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTTCATGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCg                                                                                                                           <  5:273894/149‑1 (MQ=255)                        aaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATc                                                                                                                            <  5:47047/148‑1 (MQ=255)                        aaCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCg                                                                                                                           >  2:1483/1‑149 (MQ=255)                               gAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCg                                                                                                                     <  3:124055/148‑1 (MQ=255)                                     gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGc                                                                                                                          <  6:372162/137‑1 (MQ=255)                                     gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGc                                                                                                                          >  5:372162/1‑137 (MQ=255)                                     gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGt                                                                                                              >  2:123000/1‑149 (MQ=255)                                             ccTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGa                                                                                                      >  1:189584/1‑149 (MQ=255)                                              ccggatctGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATg                                                                                                       <  5:314600/140‑1 (MQ=255)                                                gCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                                   >  7:279364/1‑149 (MQ=255)                                                 ctggTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                                   <  7:28652/146‑1 (MQ=255)                                                 cGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                                   <  8:279364/148‑1 (MQ=255)                                                 cGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCAc                                                                                                  <  7:164094/149‑1 (MQ=255)                                                  gggTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACt                                                                                                 <  1:334320/149‑1 (MQ=255)                                                       cTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAAc                                                                                             <  3:259021/148‑1 (MQ=255)                                                       cTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAAc                                                                                             <  1:341126/148‑1 (MQ=255)                                                       cTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGGCGGCATg                                                                                                       >  6:314600/1‑138 (MQ=255)                                                          ggTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTc                                                                                                             >  4:355659/1‑129 (MQ=255)                                                          ggTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTc                                                                                                             <  3:355659/129‑1 (MQ=255)                                                              tCGACCTGATCGAAGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCa                                                                                      <  3:37326/148‑1 (MQ=255)                                                                gACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGc                                                                                   >  4:234239/1‑149 (MQ=255)                                                                 aCCTGATCGATGTGTCCTCCGGTTGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTCGCTATCAGACGCGATTTGAAGAGCGCGTGCGCAAGGAAGCa                                                                                                                                  >  4:121906/1‑101 (MQ=255)                                                                 aCCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCa                                                                                                                                  <  3:121906/101‑1 (MQ=255)                                                                 aCCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                                  >  2:39652/1‑149 (MQ=255)                                                                  ccTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                                  >  4:182161/1‑148 (MQ=255)                                                                  ccTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                                  >  7:167064/1‑148 (MQ=255)                                                                   cTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGcc                                                                                  >  7:51935/1‑147 (MQ=255)                                                                               tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                                     <  8:167064/148‑1 (MQ=255)                                                                               tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                                     <  1:39652/148‑1 (MQ=255)                                                                               tcctccGGTGGTACCTCGGTCAACCCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCg                                                                    >  4:262457/1‑149 (MQ=255)                                                                                 ctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTa                                                                  >  7:66642/1‑149 (MQ=255)                                                                                 ctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTa                                                                  >  2:67810/1‑149 (MQ=255)                                                                                   ccGGTGGTACCTCGGTCAACGCCGAACTTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTAcc                                                                >  1:12571/1‑149 (MQ=255)                                                                                          tACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAgg                                                         >  2:292827/1‑149 (MQ=255)                                                                                           aCCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGc                                                        >  5:42307/1‑149 (MQ=255)                                                                                           aCCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGc                                                        >  7:69156/1‑149 (MQ=255)                                                                                             cTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCACAGGCCGAGCACATTCTGCGTACCGGGCAGGcc                                                       >  8:166650/1‑148 (MQ=255)                                                                                              tCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGa                                                     >  8:352474/1‑149 (MQ=255)                                                                                                    aaCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCTGACGTGAt                                               >  8:126324/1‑149 (MQ=255)                                                                                                    aaCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGa                                                <  8:51935/148‑1 (MQ=255)                                                                                                     aCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATc                                              <  1:123000/149‑1 (MQ=255)                                                                                                         cGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTcc                                          >  5:312600/1‑149 (MQ=255)                                                                                                         cGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTcc                                          >  4:330239/1‑149 (MQ=255)                                                                                                          gAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCt                                         <  8:69156/149‑1 (MQ=255)                                                                                                              ttCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCt                                     >  1:144274/1‑149 (MQ=255)                                                                                                                 cTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                                  <  7:166650/149‑1 (MQ=255)                                                                                                                  tACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                                  >  5:201844/1‑148 (MQ=255)                                                                                                                  tACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                                  >  6:336147/1‑148 (MQ=255)                                                                                                                         ccTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAgctgct                          >  1:117835/1‑149 (MQ=255)                                                                                                                           tGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCtgctgc                        >  4:300035/1‑149 (MQ=255)                                                                                                                                aTCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGAt                   >  1:12031/1‑149 (MQ=255)                                                                                                                                 tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGa                    <  3:182161/147‑1 (MQ=255)                                                                                                                                 tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGAt                   <  2:13769/148‑1 (MQ=255)                                                                                                                                 tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATc                  <  1:205260/149‑1 (MQ=255)                                                                                                                                  cAGACGCGCTTTGCATAGCGCGTGCGCAAGGAAGCATAGATCGCAACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATc                  <  1:354114/148‑1 (MQ=255)                                                                                                                                   gcaCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCATTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGCCGTGCTCTTCCTGGCGCGGGCGCAGCGGCGCGAGCCt                >  4:303795/3‑149 (MQ=255)                                                                                                                                   gcaCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCATTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCt                >  6:342415/3‑149 (MQ=255)                                                                                                                                   aGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCt                >  2:204414/1‑149 (MQ=255)                                                                                                                                    caCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCt                >  8:159640/2‑148 (MQ=255)                                                                                                                                      cgcgCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTAc             <  3:262457/149‑1 (MQ=255)                                                                                                                                       gcgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAg                                                                               >  4:209409/1‑82 (MQ=255)                                                                                                                                       gcgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAg                                                                               <  3:209409/82‑1 (MQ=255)                                                                                                                                        cgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTg           <  7:352474/149‑1 (MQ=255)                                                                                                                                           tttGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGcc        >  7:53215/1‑149 (MQ=255)                                                                                                                                           tttGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGcc        >  3:256180/1‑149 (MQ=255)                                                                                                                                              gCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGccac      <  3:300035/148‑3 (MQ=255)                                                                                                                                              gCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGc      <  1:204414/148‑1 (MQ=255)                                                                                                                                              gCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCt     >  3:152819/1‑149 (MQ=255)                                                                                                                                               cAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCTg    >  4:158293/1‑149 (MQ=255)                                                                                                                                                agagCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCTg    >  7:254112/1‑148 (MQ=255)                                                                                                                                                agagCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCTg    >  3:40400/1‑148 (MQ=255)                                                                                                                                                 gagCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCTGCa  <  1:67810/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGGCCGCTGCA  >  NC_002947/1684133‑1684425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑